LOCUS       sequence-228450-        6813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  phagemid containing all components for assembly of a DNA origami 
            nanorod (10-helix bundle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz 
            H
  TITLE     Biotechnological mass production of DNA origami.
  JOURNAL   Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650.
  PUBMED    29219963
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-12-6- 6"; volume: "552"; issue: "7683"; pages:
            "84-87"
COMMENT     Sequence Label: pBS_nanorod_105b
FEATURES             Location/Qualifiers
     source          1..6813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      844..1432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1333..1352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1586..1603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      6040..6495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6127..6146)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6337..6358
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6521..6625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6626..6694,6695..6813,1..673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
       61 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      121 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      181 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      241 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      301 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      361 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      421 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      481 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      541 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      601 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      661 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
      721 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      781 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      841 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      901 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      961 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     1021 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1081 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1141 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1201 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1261 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1321 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1381 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1441 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1501 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1561 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1621 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taacttaatt gcacgttgaa
     1681 gcgttacctg ttaggtaacg tagttgagct gtgcaattaa ttttttaaga gtatagttga
     1741 gctgtcacag aatgtgacgt tgaagtactc ttagaagtgt cccaactaca ctagaaggac
     1801 agtggcgaga ggattacgcg cctagatcaa ctttaatgtt gactcgtgca cccaacatgc
     1861 tttttagctc acgttgaagc gttacctgtt aggtaacgta gttgagctgt gagctaaatt
     1921 ttttcaatgt gtagttgagc tgtcacagaa tgtgacgttg aagcacattg agggctgcta
     1981 ttaagacacg acttatccct ttctcaaaag gccagcaaag cgatctggcc ccaatagggg
     2041 aacaagaggc agaacatatc aaagcgacgt tgaagcgtta cctgttaggt aacgtagttg
     2101 agctgtcgct ttgatttttt cggtaagtag ttgagctgtc acagaatgtg acgttgaagc
     2161 ttaccgagaa tagacacccg ccttacagcg aggcgaaggg ctttaaatca atctagagca
     2221 tcataccagg cgtttcgttc ttggcgccgc aaccacctgt atgcacgttg aagcgttacc
     2281 tgttaggtaa cgtagttgag ctgtgcatac agttcctgcg gtctatagtt gagctgtcac
     2341 agaatgtgac gttgaagtag accgcgaaaa atgacgggga aagcctggcg aataactacg
     2401 ttgcctgact cccggggata ttctcatagc tcactaacta ttgtgctgta gagctccgtc
     2461 tacgttgaag cgttacctgt taggtaacgt agttgagctg tagacggagt ttttttggtc
     2521 gctagttgag ctgtcacaga atgtgacgtt gaaggcgacc aaactctcag ggttattgtc
     2581 tgatttatcg cgtccggcgg tgctacagac ccctggtccg cccccctgac aagtataaaa
     2641 ccagcattta tcaaggatac gttgaagcgt tacctgttag gtaacgtagt tgagctgtat
     2701 ccttgatttt ttgccctgat agttgagctg tcacagaatg tgacgttgaa gtcagggcat
     2761 aaatcgcgtt aatattttgc gcggggatta agttgcgcct tatccgggct gtagtatcca
     2821 cagaatcacg cgtatgtttg tcattgtaaa aaagaacgtt gaagcgttac ctgttaggta
     2881 acgtagttga gctgttcttt ttttttttca ccgaagtagt tgagctgtca cagaatgtga
     2941 cgttgaagct tcggtgtttg gtccatccaa aaaggatctt cacagaaaaa tgtttgcaag
     3001 cagcagtatt tcattcagaa agcggtctgt gactggtgat aacccaatac tcacgttgaa
     3061 gcgttacctg ttaggtaacg tagttgagct gtgagtattg tttttcgctc ctttagttga
     3121 gctgtcacag aatgtgacgt tgaagaagga gcgggaaggc aatgatgagg cacctatctc
     3181 aaggccacgg atacctgtcc ggccactggt gcgggaggga agcactatta aagaaccagt
     3241 ttggttccgc acgttgaagc gttacctgtt aggtaacgta gttgagctgt gcggaacctt
     3301 ttttcctgta gtagttgagc tgtcacagaa tgtgacgttg aagctacagg aagttggctg
     3361 cataattctc tttcaccaaa tgccgcaaaa aaaattgttg tgtcacccag ttaccttcgg
     3421 aaaccactga tcttttctac gttaagggag ctagacgttg aagcgttacc tgttaggtaa
     3481 cgtagttgag ctgtctagct cctttttcca cgccttagtt gagctgtcac agaatgtgac
     3541 gttgaagagg cgtgggcgct cttccgatac ggtgtatctc agttcggcga ccgctgggta
     3601 accctaaaca ctacgtgaac caccgaaatt cgcgttacgt tgaagcgtta cctgttaggt
     3661 aacgtagttg agctgtaacg cgaacctttc ctcatgatag ttgagctgtc acagaatgtg
     3721 acgttgaagt catgaggatc cttcgctggt agcggtggct gaaggctcgt ccctccgaat
     3781 gccatccgta agtgatctta gggcgacacg gaatccgcct atggcttggt atctacgttg
     3841 aagcgttacc tgttaggtaa cgtagttgag ctgtagatac catttttgca agttttagtt
     3901 gagctgtcac agaatgtgac gttgaagaaa cttgcatagg caagctccct cgtgcgtatg
     3961 tacattcgct gtagcgtctt gcccggcgtc ggaaaacgga tacatatttg agacccacgc
     4021 tgcgcattag cacgttgaag cgttacctgt taggtaacgt agttgagctg tgctaatgct
     4081 tttttgaacg aatagttgag ctgtcacaga atgtgacgtt gaagttcgtt catgtgagcc
     4141 cttcgggaag cgcccggtac gccagcggcg aacccaacgt caaagggaga taggaaagtg
     4201 ccacctaagt gtagaagggg gacgtcttga cgttgaagcg ttacctgtta ggtaacgtag
     4261 ttgagctgtc aagacgtttt tttgcgtcga tagttgagct gtcacagaat gtgacgttga
     4321 agtcgacgca tgaagtcgag cgcccttttt gatccagttc gatggtactc accatgttgt
     4381 gcaaactccg gtgtcctgcc ttttaaatta aaatcaagtc aaacccgacg ttgaagcgtt
     4441 acctgttagg taacgtagtt gagctgtcgg gtttgttttt tctttttata gttgagctgt
     4501 cacagaatgt gacgttgaag taaaaagaat cagtaccgcg tatgtattaa gtgctcatca
     4561 ttaatacgga gggcgctggc aagcattcag gctcaccagt taccaatgct tgccgcgttg
     4621 ttccgacgtt gaagcgttac ctgttaggta acgtagttga gctgtcggaa caatttttgg
     4681 tcctgttagt tgagctgtca cagaatgtga cgttgaagac aggacctacg gcgatcaagt
     4741 gatcccacca agtcattctg ctgttgacaa tattattgaa gccagccggc ttaataagtg
     4801 gtggcctaat ataaagacaa aaacgttgaa gcgttacctg ttaggtaacg tagttgagct
     4861 gtttttgtct ccttcagcag ggttagttga gctgtcacag aatgtgacgt tgaagaccct
     4921 gctaacagga actgttgggc gctgataata ccgcgccact ttaatagaaa aataaacaag
     4981 tgctggcgat cggcagcagc cactggcgct tacggaaccg acgttgaagc gttacctgtt
     5041 aggtaacgta gttgagctgt cggttccgtt tttacagagc gtagttgagc tgtcacagaa
     5101 tgtgacgttg aagcgctctg ttttttaagg atctcaagaa attatcagtc tattgggaat
     5161 acagcatctt ttacttactg tctcgttgtc agaagtcatc gtggccggga attttggacg
     5221 ttgaagcgtt acctgttagg taacgtagtt gagctgtcca aaattttttt gcctcgctta
     5281 gttgagctgt cacagaatgt gacgttgaag agcgaggctc tcctgctggc gtttttcgtc
     5341 tgacggctcc acatgagcgt tcttcggggc gagagttgcg tcacgctgcg cgttacaggg
     5401 cagcaattat gagtacgttg aagcgttacc tgttaggtaa cgtagttgag ctgtactcat
     5461 aacctttcca ttggatagtt gagctgtcac agaatgtgac gttgaagtcc aatggcgcta
     5521 cgcaggaaag aacatccata gatacggtcc ccgagttgag tgttgttcgt ggactgtggc
     5581 gagaaaggac ctcttctacc atctgtctat acgttgaagc gttacctgtt aggtaacgta
     5641 gttgagctgt atagacagtt tttaggctga atagttgagc tgtcacagaa tgtgacgttg
     5701 aagttcagcc tgtaggtact caaaggcggt acttcctcgc caacgttaaa atcggcaaaa
     5761 tcccttgatg gccgggagcc cccgattcaa ggcgacgttg aagcgttacc tgttaggtaa
     5821 cgtagttgag ctgtcgcctt gatttttgct cacattagtt gagctgtcac agaatgtgac
     5881 gttgaagatg tgagctacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     5941 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6001 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6061 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6121 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6181 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6241 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6301 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6361 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6421 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6481 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6601 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6661 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6721 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6781 taagatcctt gagagttttc gccccgaaga acg
//
