LOCUS       sequence-228454-        3246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for full-site selection assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVC_CRISPR_Cat
FEATURES             Location/Qualifiers
     source          1..3246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        41..143
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             233..892
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(299..318)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      913..1007
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     1104..1126
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1164..1182)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1250..1354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1355..1423,1424..2215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1573..1592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2386..2974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2875..2894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3128..3145
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgagcgcaac gcaatcctct agaggatcca aatgagacgt tgatcggcac gtaagaggtt
       61 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      121 tttcaggagc taaggaagct aaaatgtccc cattattgga agggtttata aggaaagtaa
      181 agaagacctg ctttctagac gtccattgtt gatccgggaa ggatcggtct tcatggagaa
      241 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      301 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      361 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      421 tgcccgcctg atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt
      481 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      541 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      601 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      661 tttcgtgtca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      721 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      781 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      841 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aatttgatat
      901 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      961 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcct taagccagcc
     1021 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     1081 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     1141 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1201 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1621 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1861 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2221 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3121 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     3181 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     3241 gggcag
//
