LOCUS       sequence-228470-        8439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hAID-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198.
  PUBMED    30125268
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198."
COMMENT     Sequence Label: pCMV-hAID-BE3
FEATURES             Location/Qualifiers
     source          1..8439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        109..488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        489..692
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     643..663
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..753
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        734..752
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      760..769
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             766..1359
                     /codon_start=1
                     /gene="AICDA"
                     /product="human activation-induced cytidine deaminase 
                     (Dickerson et al., 2003)"
                     /label=AID
                     /translation="MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLD
                     FGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNL
                     SLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWE
                     GLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL"
     CDS             join(1408..4806,4807..4809,4810..5406,5407,5408..5410,
                     5411..5412,5413..5415,5416..5508)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1408..1431,1432..1434,1435..5508)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5521..5769
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5782..5802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5811..5828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5851..5868)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5857..6081
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6152..6168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6152..6168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6165..6187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6176..6192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6200..6206,6207..6224,6225..6230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6245..6266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6383..6400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6554..7142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6634..6653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7313..8104,8105..8173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7936..7955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8174..8278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8357..8376)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
       61 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
      121 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      181 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      241 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      301 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      361 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      421 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      481 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      541 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      601 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      661 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctaga
      721 gatccgcggc cgctaatacg actcactata gggagagccg ccaccatgga cagcctcttg
      781 atgaaccgga ggaagtttct ttaccaattc aaaaatgtcc gctgggctaa gggtcggcgt
      841 gagacctacc tgtgctacgt agtgaagagg cgtgacagtg ctacatcctt ttcactggac
      901 tttggttatc ttcgcaataa gaacggctgc cacgtggaat tgctcttcct ccgctacatc
      961 tcggactggg acctagaccc tggccgctgc taccgcgtca cctggttcac ctcctggagc
     1021 ccctgctacg actgtgcccg acatgtggcc gactttctgc gagggaaccc caacctcagt
     1081 ctgaggatct tcaccgcgcg cctctacttc tgtgaggacc gcaaggctga gcccgagggg
     1141 ctgcggcggc tgcaccgcgc cggggtgcaa atagccatca tgaccttcaa agattatttt
     1201 tactgctgga atacttttgt agaaaaccac gaaagaactt tcaaagcctg ggaagggctg
     1261 catgaaaatt cagttcgtct ctccagacag cttcggcgca tccttttgcc cctgtatgag
     1321 gttgatgact tacgagacgc atttcgtact ttgggactta gcggcagcga gactcccggg
     1381 acctcagagt ccgccacacc cgaaagtgat aaaaagtatt ctattggttt agccatcggc
     1441 actaattccg ttggatgggc tgtcataacc gatgaataca aagtaccttc aaagaaattt
     1501 aaggtgttgg ggaacacaga ccgtcattcg attaaaaaga atcttatcgg tgccctccta
     1561 ttcgatagtg gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg gagaaggtat
     1621 acacgtcgca agaaccgaat atgttactta caagaaattt ttagcaatga gatggccaaa
     1681 gttgacgatt ctttctttca ccgtttggaa gagtccttcc ttgtcgaaga ggacaagaaa
     1741 catgaacggc accccatctt tggaaacata gtagatgagg tggcatatca tgaaaagtac
     1801 ccaacgattt atcacctcag aaaaaagcta gttgactcaa ctgataaagc ggacctgagg
     1861 ttaatctact tggctcttgc ccatatgata aagttccgtg ggcactttct cattgagggt
     1921 gatctaaatc cggacaactc ggatgtcgac aaactgttca tccagttagt acaaacctat
     1981 aatcagttgt ttgaagagaa ccctataaat gcaagtggcg tggatgcgaa ggctattctt
     2041 agcgcccgcc tctctaaatc ccgacggcta gaaaacctga tcgcacaatt acccggagag
     2101 aagaaaaatg ggttgttcgg taaccttata gcgctctcac taggcctgac accaaatttt
     2161 aagtcgaact tcgacttagc tgaagatgcc aaattgcagc ttagtaagga cacgtacgat
     2221 gacgatctcg acaatctact ggcacaaatt ggagatcagt atgcggactt atttttggct
     2281 gccaaaaacc ttagcgatgc aatcctccta tctgacatac tgagagttaa tactgagatt
     2341 accaaggcgc cgttatccgc ttcaatgatc aaaaggtacg atgaacatca ccaagacttg
     2401 acacttctca aggccctagt ccgtcagcaa ctgcctgaga aatataagga aatattcttt
     2461 gatcagtcga aaaacgggta cgcaggttat attgacggcg gagcgagtca agaggaattc
     2521 tacaagttta tcaaacccat attagagaag atggatggga cggaagagtt gcttgtaaaa
     2581 ctcaatcgcg aagatctact gcgaaagcag cggactttcg acaacggtag cattccacat
     2641 caaatccact taggcgaatt gcatgctata cttagaaggc aggaggattt ttatccgttc
     2701 ctcaaagaca atcgtgaaaa gattgagaaa atcctaacct ttcgcatacc ttactatgtg
     2761 ggacccctgg cccgagggaa ctctcggttc gcatggatga caagaaagtc cgaagaaacg
     2821 attactccat ggaattttga ggaagttgtc gataaaggtg cgtcagctca atcgttcatc
     2881 gagaggatga ccaactttga caagaattta ccgaacgaaa aagtattgcc taagcacagt
     2941 ttactttacg agtatttcac agtgtacaat gaactcacga aagttaagta tgtcactgag
     3001 ggcatgcgta aacccgcctt tctaagcgga gaacagaaga aagcaatagt agatctgtta
     3061 ttcaagacca accgcaaagt gacagttaag caattgaaag aggactactt taagaaaatt
     3121 gaatgcttcg attctgtcga gatctccggg gtagaagatc gatttaatgc gtcacttggt
     3181 acgtatcatg acctcctaaa gataattaaa gataaggact tcctggataa cgaagagaat
     3241 gaagatatct tagaagatat agtgttgact cttaccctct ttgaagatcg ggaaatgatt
     3301 gaggaaagac taaaaacata cgctcacctg ttcgacgata aggttatgaa acagttaaag
     3361 aggcgtcgct atacgggctg gggacgattg tcgcggaaac ttatcaacgg gataagagac
     3421 aagcaaagtg gtaaaactat tctcgatttt ctaaagagcg acggcttcgc caataggaac
     3481 tttatgcagc tgatccatga tgactcttta accttcaaag aggatataca aaaggcacag
     3541 gtttccggac aaggggactc attgcacgaa catattgcga atcttgctgg ttcgccagcc
     3601 atcaaaaagg gcatactcca gacagtcaaa gtagtggatg agctagttaa ggtcatggga
     3661 cgtcacaaac cggaaaacat tgtaatcgag atggcacgcg aaaatcaaac gactcagaag
     3721 gggcaaaaaa acagtcgaga gcggatgaag agaatagaag agggtattaa agaactgggc
     3781 agccagatct taaaggagca tcctgtggaa aatacccaat tgcagaacga gaaactttac
     3841 ctctattacc tacaaaatgg aagggacatg tatgttgatc aggaactgga cataaaccgt
     3901 ttatctgatt acgacgtcga tcacattgta ccccaatcct ttttgaagga cgattcaatc
     3961 gacaataaag tgcttacacg ctcggataag aaccgaggga aaagtgacaa tgttccaagc
     4021 gaggaagtcg taaagaaaat gaagaactat tggcggcagc tcctaaatgc gaaactgata
     4081 acgcaaagaa agttcgataa cttaactaaa gctgagaggg gtggcttgtc tgaacttgac
     4141 aaggccggat ttattaaacg tcagctcgtg gaaacccgcc aaatcacaaa gcatgttgca
     4201 cagatactag attcccgaat gaatacgaaa tacgacgaga acgataagct gattcgggaa
     4261 gtcaaagtaa tcactttaaa gtcaaaattg gtgtcggact tcagaaagga ttttcaattc
     4321 tataaagtta gggagataaa taactaccac catgcgcacg acgcttatct taatgccgtc
     4381 gtagggaccg cactcattaa gaaatacccg aagctagaaa gtgagtttgt gtatggtgat
     4441 tacaaagttt atgacgtccg taagatgatc gcgaaaagcg aacaggagat aggcaaggct
     4501 acagccaaat acttctttta ttctaacatt atgaatttct ttaagacgga aatcactctg
     4561 gcaaacggag agatacgcaa acgaccttta attgaaacca atggggagac aggtgaaatc
     4621 gtatgggata agggccggga cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc
     4681 aacatagtaa agaaaactga ggtgcagacc ggagggtttt caaaggaatc gattcttcca
     4741 aaaaggaata gtgataagct catcgctcgt aaaaaggact gggacccgaa aaagtacggt
     4801 ggcttcgata gccctacagt tgcctattct gtcctagtag tggcaaaagt tgagaaggga
     4861 aaatccaaga aactgaagtc agtcaaagaa ttattgggga taacgattat ggagcgctcg
     4921 tcttttgaaa agaaccccat cgacttcctt gaggcgaaag gttacaagga agtaaaaaag
     4981 gatctcataa ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg
     5041 atgttggcta gcgccggaga gcttcaaaag gggaacgaac tcgcactacc gtctaaatac
     5101 gtgaatttcc tgtatttagc gtcccattac gagaagttga aaggttcacc tgaagataac
     5161 gaacagaagc aactttttgt tgagcagcac aaacattatc tcgacgaaat catagagcaa
     5221 atttcggaat tcagtaagag agtcatccta gctgatgcca atctggacaa agtattaagc
     5281 gcatacaaca agcacaggga taaacccata cgtgagcagg cggaaaatat tatccatttg
     5341 tttactctta ccaacctcgg cgctccagcc gcattcaagt attttgacac aacgatagat
     5401 cgcaaacgat acacttctac caaggaggtg ctagacgcga cactgattca ccaatccatc
     5461 acgggattat atgaaactcg gatagatttg tcacagcttg ggggtgactc tggtggttct
     5521 actaatctgt cagatattat tgaaaaggag accggtaagc aactggttat ccaggaatcc
     5581 atcctcatgc tcccagagga ggtggaagaa gtcattggga acaagccgga aagcgatata
     5641 ctcgtgcaca ccgcctacga cgagagcacc gacgagaatg tcatgcttct gactagcgac
     5701 gcccctgaat acaagccttg ggctctggtc atacaggata gcaacggtga gaacaagatt
     5761 aagatgctct ctggtggttc tcccaagaag aagaggaaag tctaaccggt catcatcacc
     5821 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5881 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5941 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6001 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6061 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     6121 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6181 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     6241 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6301 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8281 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     8341 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8401 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagca
//
