LOCUS       sequence-228481-        6503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            flagellin promoter driven mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2G_PflaB-mCeruleanBb
FEATURES             Location/Qualifiers
     source          1..6503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1129..1146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1129..1145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1138..1160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1712..2245
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      2348..2936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2837..2856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6413..6434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6449..6454,6455..6472,6473..6479)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6487..6503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6492..6503,1..11)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tttcacacag gaaacagcta tgacatgatt acgaattcga gctctgtctg tcgcctcttg
       61 tggcttccgg aggagttatt tataataaaa taaagaatta cgacttgaaa atttttaatc
      121 aaaaaaataa agttgaagat tataaaaaga agtacgaaga tagagagaga aaagtggaag
      181 gtgaacttaa taccttggat tttaccgtta agcgcatgaa agatcaagaa aatacattaa
      241 aggcttttga ttttaatcaa agaaataaat aataataatt atttttaatg ctattgctat
      301 ttgcgtttct ttttttttaa tttttgtgct attcttttta acaggcaaaa ggatttgcca
      361 aagtcagaaa tttaaatttt atcatggagg aatgaggatc catggttagt aaaggtgaag
      421 aattatttac tggtgttgtt cctattttag ttgaattaga tggtgatgtt aatggtcata
      481 aatttagtgt tagtggtgaa ggtgaaggtg atgctactta tggtaaatta actttaaaat
      541 ttatttgtac tactggtaaa ttacctgttc cttggcctac tttagttact actttaactt
      601 ggggtgttca atgttttgct agatatcctg atcatatgaa acaacatgat ttttttaaaa
      661 gtgctatgcc tgaaggttat gttcaagaaa ggactatttt cttcaaagat gatggtaatt
      721 ataaaactag agctgaagtt aaatttgaag gtgatacttt agttaataga attgaattaa
      781 aaggtattga ttttaaagaa gatggtaata ttttaggtca taaattagaa tataatgcta
      841 ttagtgataa tgtttatatt acagctgata aacaaaaaaa tggtattaaa gctaatttta
      901 aaattagaca taatattgaa gatggtagtg ttcaattagc tgatcattat caacaaaata
      961 ctcctattgg tgatggtcct gttttattac ctgataatca ttatttaagt actcaaagta
     1021 aattaagtaa agatcctaat gaaaaaagag atcatatggt tttattagaa tttgttactg
     1081 ctgctggtat tactttaggt atggatgaat tatataaata agcttggcac tggccgtcgt
     1141 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1201 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     1261 gttgcgcagc ctgaatggcg aatggcgcgg ccgccctagg ttaatacccg agcttcaagg
     1321 aagatttcct attaaggttg aacttaagag cttaagcata gacgatttga aaaaaatttt
     1381 aaaacaaaca aaaaattctt taataaaaca atatgttgcg atgtttaagg tttatgattt
     1441 agatttaaag tttagtgagg aagctataga tagaattgca gagcttactt ttaatatgaa
     1501 tcttgagagt gaaaatcttg gtgccagaag acttcacggt gttatggaaa tagtgcttgc
     1561 agatcttttt tttgaagtgc ctggcagtaa gttgaaaaaa tttgaaataa acttggacta
     1621 tgttaataaa aaaatacaaa ttaacgaaca aaaagatttg aattattata taatttagtt
     1681 aaaagcaatt ttaaatgagg gaggtttcca tatgttacgc agcagcaacg atgttacgca
     1741 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg
     1801 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga
     1861 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt
     1921 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc
     1981 tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga
     2041 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct
     2101 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga
     2161 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga
     2221 tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct tacgcgtcca
     2281 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2341 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2401 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2461 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2521 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2581 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2641 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2701 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2761 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2821 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2881 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2941 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3001 ttcctgcact agatccccgg caagttcatc cttagggttt tcatatctct taaaaagtca
     3061 atttctgctt gattaatttc ttgtggatta ttgtttttgc ggtttttcat tttttatctc
     3121 cttgagtatt gaattttttc ttatatataa aatatagcaa aaactatttt tgccaacttt
     3181 tttacaaaaa attttttatt aaaacactta gggctttgct aaattctctt ttaaaagaac
     3241 ttagtaaagc cctatggatt taagaactga ttatacttac gtagtaaaaa tactacagat
     3301 attaatagta tagaccatat caaaatcata atcaatttat atttatctaa aattgtggtc
     3361 catacaaaat acaaaaaaat taggtttttc taaattctat tttaaaagaa cttagtaaca
     3421 cctaatctct aaattactta caaaattcta aaattagatt tttagttctt catatttctt
     3481 taaaagtttt ttaagaaaat ctttttgatt ttcaaaaatt tcatgcatca taaaactagt
     3541 aaatttggat ttttttttgt aaaaatcata actttcttgg gttttaagtt gaaatcttaa
     3601 tggttttatt ggattttgcc tagatttttt taacgccgga ctttctttat cttttataaa
     3661 attataagaa tttttaatac cattttctat taaataatct tcttttataa gcccctcttg
     3721 cagtgcagtt gctaatctta aatacttata cgcttgagtt tttgctacat caaagtcttt
     3781 tataaaatca agaaaagttt tataaccatc aaatttataa tattgattat cttttatttc
     3841 ttttaaaatt ttaatatttt ccaccctgtg gaatatttct tttttgaaat tgtcatttaa
     3901 cttttccttt agtgtcttgt aataagaaat tttactttca ttaatcatta aattttgttc
     3961 aacataagta tctatctttt ttgttaaaac tttaacaaca cgtttttttt ccataaaaat
     4021 tttcctttat ttttgcaaca ttttccttaa ttgatataat tgttactatt ttagattttt
     4081 taatatccac ttaagaatta atccctcact agaatatttt ttattataga attcatatac
     4141 atgtttttct aaatctataa atttatcaac aattgcttta ttatagggtg tatctatctt
     4201 ttcttttctt aataatcgtc tcatattctt taaataacaa caaacactgc cggttttaaa
     4261 cctaaattct atgtaatata tttttgaaaa agtatataca ttttcaaccc cgtttatttc
     4321 atactttaca aaaacattat ttattggttt tctataacca tagtaaatgc ctaaaaattt
     4381 gtccccgtct ttcataggat atagataaag tttttgaatc tctgatttat ctaataattt
     4441 ttcaaaaaaa attatacacc gcttttgttt gttactaact actctatgca atgcctttaa
     4501 tatttttgtg tgatatatat ttttatcacc ttttttttct ttcagcagga agatactttc
     4561 ttttggaaat ttaatttttt gttcatttat tcttgcagcc ttaagtccga ttttcatatt
     4621 taatccttat atagcctttc tatttaattc ttttaaactc aataaacttt tttggttttt
     4681 cattagttct aataattcgc aataatatgt gcaaaacact ttattgtatt ctattttctt
     4741 ttggttattc aaatattctt ttataattgg ctttagaagt tcaatattta cttctttttt
     4801 tagttgttca ataaggatat tgaaaatgtt tgtttttaaa tcttgataat tttgttgaga
     4861 attttctttt tttctttcaa ttgacttctc taatttacgc tttatattgt ttaaatcgct
     4921 atatttatga ctttcaataa taaaatgagg cttaaatttg taattttcgt atattttttg
     4981 gaaacttgtt tctaattgtt tagaattata ttcatttttt tcaaattctt tttgagtgtt
     5041 gcatagaatt tcctttaatt tttcttgttt ttctttcata caagatttag taaaatgtat
     5101 atttttattt tttagtaatt taatttctat tatttttatg attttaagta ttttgattat
     5161 attatcttta tcaatattta aattgaataa aattgtaaga aaaattgaaa gaattttttt
     5221 acataaaaag ttacatttat tgaagtaatt tcttacttga tacttttcta tttcattaat
     5281 ctttctttct tcttttatat tatttttatt atttaaacac tcctttaaat ctacactccc
     5341 atttttagta aatctatctt taaggcccct tgaaactcta ttttgaaatc ttaaatcttt
     5401 tttttcttga aagtatttat agattttttg gtaacattct ttttttgaaa aattaagttg
     5461 atagtatatt tcggtcccca aatttacccc taagtgtttg tgataattag ttgtgacttt
     5521 aaattctttt tctaatttgt aaaggtaatt ttgcagtgtt tttagtttaa cgggtgtttg
     5581 actatttctt ttaaggtttt cattaaagta atagagtatg tttttttgtg tgtatttctt
     5641 atatgtttta tttatgtacg ctagtgttga gataaggact actaatttat actgtaattt
     5701 attgtagcaa gttgtggttt tactggtttt gtttatatta gataagtttt ccatttatag
     5761 ctccttgaat accggatttt ttatctataa agtatatata tcacaatttt gatttaaaag
     5821 taaattcttt ttttaaaaat acaaaaattt tttataaatt atatagacca tgatattgta
     5881 ttaatactaa ttgattttaa tttaaggttg aactaaaata aacatagttt tgtagaaaat
     5941 aatttttcat tattttgcta cttgatattg gatcgtaaaa atattagggc tttacaaagt
     6001 tcttttaaaa gagaatttag taaagcccta agtcttttaa taaaaaattt tttgtaaaaa
     6061 agttggcaaa aatagttttt gctatatatt tatatataag aaaaaattca atactcaagg
     6121 agataaaaaa tggaaaactt aaacaataat ccacaaaaaa ttaatcaaat aaataagcaa
     6181 actaatttta ttggttttga aaattttata gaacttgata atcaagttaa tcaaactaaa
     6241 gaaaaaagta aattaagtaa gataggtaag aaattacccg gaataagcag tcaagtctag
     6301 tgcggccgcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6361 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     6421 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     6481 gtggaattgt gagcggataa caa
//
