LOCUS       sequence-228484-        6503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes 
            flagellin promoter driven mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Takacs CN, Kloos ZA, Scott M, Rosa PA, Jacobs-Wagner C
  TITLE     Fluorescent Proteins, Promoters, and Selectable Markers for 
            Applications in the Lyme Disease Spirochete Borrelia burgdorferi
  JOURNAL   Appl Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18.
  PUBMED    30315081
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2018 Oct 12. pii: AEM.01824-18. doi: 
            10.1128/AEM.01824-18."
COMMENT     Sequence Label: pBSV2G_PflaB-mEGFPBb
FEATURES             Location/Qualifiers
     source          1..6503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    8..24
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13..35
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     32..48
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     32..48
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1153..1170)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1153..1169)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1162..1184)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             1736..2269
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      2372..2960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2861..2880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6437..6458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6473..6478,6479..6496,6497..6503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
       61 tcgagctctg tctgtcgcct cttgtggctt ccggaggagt tatttataat aaaataaaga
      121 attacgactt gaaaattttt aatcaaaaaa ataaagttga agattataaa aagaagtacg
      181 aagatagaga gagaaaagtg gaaggtgaac ttaatacctt ggattttacc gttaagcgca
      241 tgaaagatca agaaaataca ttaaaggctt ttgattttaa tcaaagaaat aaataataat
      301 aattattttt aatgctattg ctatttgcgt ttcttttttt ttaatttttg tgctattctt
      361 tttaacaggc aaaaggattt gccaaagtca gaaatttaaa ttttatcatg gaggaatgag
      421 gatccatggt tagtaaaggt gaagaattat ttactggtgt tgttcctatt ttagttgaat
      481 tagatggtga tgttaatggt cataaattta gtgttagtgg tgaaggtgaa ggtgatgcta
      541 cttatggtaa attaacttta aaatttattt gtactactgg taaattacct gttccttggc
      601 ctactttagt tactacttta acttatggtg ttcaatgttt tagtagatat cctgatcata
      661 tgaaacaaca tgattttttt aaaagtgcta tgcctgaagg ttatgttcaa gaaaggacta
      721 ttttcttcaa agatgatggt aattataaaa ctagagctga agttaaattt gaaggtgata
      781 ctttagttaa tagaattgaa ttaaaaggta ttgattttaa agaagatggt aatattttag
      841 gtcataaatt agaatataat tataatagtc ataatgttta tattatggct gataaacaaa
      901 aaaatggtat taaagttaat tttaaaatta gacataatat tgaagatggt agtgttcaat
      961 tagctgatca ttatcaacaa aatactccta ttggtgatgg tcctgtttta ttacctgata
     1021 atcattattt aagtactcaa agtaaattaa gtaaagatcc taatgaaaaa agagatcata
     1081 tggttttatt agaatttgtt actgctgctg gtattacttt aggtatggat gaattatata
     1141 aataagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     1201 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     1261 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcggccgccc
     1321 taggttaata cccgagcttc aaggaagatt tcctattaag gttgaactta agagcttaag
     1381 catagacgat ttgaaaaaaa ttttaaaaca aacaaaaaat tctttaataa aacaatatgt
     1441 tgcgatgttt aaggtttatg atttagattt aaagtttagt gaggaagcta tagatagaat
     1501 tgcagagctt acttttaata tgaatcttga gagtgaaaat cttggtgcca gaagacttca
     1561 cggtgttatg gaaatagtgc ttgcagatct tttttttgaa gtgcctggca gtaagttgaa
     1621 aaaatttgaa ataaacttgg actatgttaa taaaaaaata caaattaacg aacaaaaaga
     1681 tttgaattat tatataattt agttaaaagc aattttaaat gagggaggtt tccatatgtt
     1741 acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc
     1801 aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc
     1861 tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc
     1921 ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt
     1981 cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc
     2041 gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat
     2101 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
     2161 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
     2221 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca
     2281 agccgagatc ggcttacgcg tccaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3001 gctggccttt tgctcacatg ttctttcctg cactagatcc ccggcaagtt catccttagg
     3061 gttttcatat ctcttaaaaa gtcaatttct gcttgattaa tttcttgtgg attattgttt
     3121 ttgcggtttt tcatttttta tctccttgag tattgaattt tttcttatat ataaaatata
     3181 gcaaaaacta tttttgccaa cttttttaca aaaaattttt tattaaaaca cttagggctt
     3241 tgctaaattc tcttttaaaa gaacttagta aagccctatg gatttaagaa ctgattatac
     3301 ttacgtagta aaaatactac agatattaat agtatagacc atatcaaaat cataatcaat
     3361 ttatatttat ctaaaattgt ggtccataca aaatacaaaa aaattaggtt tttctaaatt
     3421 ctattttaaa agaacttagt aacacctaat ctctaaatta cttacaaaat tctaaaatta
     3481 gatttttagt tcttcatatt tctttaaaag ttttttaaga aaatcttttt gattttcaaa
     3541 aatttcatgc atcataaaac tagtaaattt ggattttttt ttgtaaaaat cataactttc
     3601 ttgggtttta agttgaaatc ttaatggttt tattggattt tgcctagatt tttttaacgc
     3661 cggactttct ttatctttta taaaattata agaattttta ataccatttt ctattaaata
     3721 atcttctttt ataagcccct cttgcagtgc agttgctaat cttaaatact tatacgcttg
     3781 agtttttgct acatcaaagt cttttataaa atcaagaaaa gttttataac catcaaattt
     3841 ataatattga ttatctttta tttcttttaa aattttaata ttttccaccc tgtggaatat
     3901 ttcttttttg aaattgtcat ttaacttttc ctttagtgtc ttgtaataag aaattttact
     3961 ttcattaatc attaaatttt gttcaacata agtatctatc ttttttgtta aaactttaac
     4021 aacacgtttt ttttccataa aaattttcct ttatttttgc aacattttcc ttaattgata
     4081 taattgttac tattttagat tttttaatat ccacttaaga attaatccct cactagaata
     4141 ttttttatta tagaattcat atacatgttt ttctaaatct ataaatttat caacaattgc
     4201 tttattatag ggtgtatcta tcttttcttt tcttaataat cgtctcatat tctttaaata
     4261 acaacaaaca ctgccggttt taaacctaaa ttctatgtaa tatatttttg aaaaagtata
     4321 tacattttca accccgttta tttcatactt tacaaaaaca ttatttattg gttttctata
     4381 accatagtaa atgcctaaaa atttgtcccc gtctttcata ggatatagat aaagtttttg
     4441 aatctctgat ttatctaata atttttcaaa aaaaattata caccgctttt gtttgttact
     4501 aactactcta tgcaatgcct ttaatatttt tgtgtgatat atatttttat cacctttttt
     4561 ttctttcagc aggaagatac tttcttttgg aaatttaatt ttttgttcat ttattcttgc
     4621 agccttaagt ccgattttca tatttaatcc ttatatagcc tttctattta attcttttaa
     4681 actcaataaa cttttttggt ttttcattag ttctaataat tcgcaataat atgtgcaaaa
     4741 cactttattg tattctattt tcttttggtt attcaaatat tcttttataa ttggctttag
     4801 aagttcaata tttacttctt tttttagttg ttcaataagg atattgaaaa tgtttgtttt
     4861 taaatcttga taattttgtt gagaattttc tttttttctt tcaattgact tctctaattt
     4921 acgctttata ttgtttaaat cgctatattt atgactttca ataataaaat gaggcttaaa
     4981 tttgtaattt tcgtatattt tttggaaact tgtttctaat tgtttagaat tatattcatt
     5041 tttttcaaat tctttttgag tgttgcatag aatttccttt aatttttctt gtttttcttt
     5101 catacaagat ttagtaaaat gtatattttt attttttagt aatttaattt ctattatttt
     5161 tatgatttta agtattttga ttatattatc tttatcaata tttaaattga ataaaattgt
     5221 aagaaaaatt gaaagaattt ttttacataa aaagttacat ttattgaagt aatttcttac
     5281 ttgatacttt tctatttcat taatctttct ttcttctttt atattatttt tattatttaa
     5341 acactccttt aaatctacac tcccattttt agtaaatcta tctttaaggc cccttgaaac
     5401 tctattttga aatcttaaat cttttttttc ttgaaagtat ttatagattt tttggtaaca
     5461 ttcttttttt gaaaaattaa gttgatagta tatttcggtc cccaaattta cccctaagtg
     5521 tttgtgataa ttagttgtga ctttaaattc tttttctaat ttgtaaaggt aattttgcag
     5581 tgtttttagt ttaacgggtg tttgactatt tcttttaagg ttttcattaa agtaatagag
     5641 tatgtttttt tgtgtgtatt tcttatatgt tttatttatg tacgctagtg ttgagataag
     5701 gactactaat ttatactgta atttattgta gcaagttgtg gttttactgg ttttgtttat
     5761 attagataag ttttccattt atagctcctt gaataccgga ttttttatct ataaagtata
     5821 tatatcacaa ttttgattta aaagtaaatt ctttttttaa aaatacaaaa attttttata
     5881 aattatatag accatgatat tgtattaata ctaattgatt ttaatttaag gttgaactaa
     5941 aataaacata gttttgtaga aaataatttt tcattatttt gctacttgat attggatcgt
     6001 aaaaatatta gggctttaca aagttctttt aaaagagaat ttagtaaagc cctaagtctt
     6061 ttaataaaaa attttttgta aaaaagttgg caaaaatagt ttttgctata tatttatata
     6121 taagaaaaaa ttcaatactc aaggagataa aaaatggaaa acttaaacaa taatccacaa
     6181 aaaattaatc aaataaataa gcaaactaat tttattggtt ttgaaaattt tatagaactt
     6241 gataatcaag ttaatcaaac taaagaaaaa agtaaattaa gtaagatagg taagaaatta
     6301 cccggaataa gcagtcaagt ctagtgcggc cgcgcccaat acgcaaaccg cctctccccg
     6361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6481 ttatgcttcc ggctcgtatg ttg
//
