LOCUS       sequence-228490-        9894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged CYFIP2 expression construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Pathania M, Davenport EC, Muir J, Sheehan DF, Lopez-Domenech G, 
            Kittler JT
  TITLE     The autism and schizophrenia associated gene CYFIP1 is critical for 
            the maintenance of dendritic complexity and the stabilization of 
            mature spines.
  JOURNAL   Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16.
  PUBMED    24667445
REFERENCE   2  (bases 1 to 9894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transl 
            Psychiatry."; date: "2014-03-25"; volume: "4:e374. doi"; pages: " 
            10.1038/tp.2014.16"
COMMENT     Sequence Label: pDEST47-CYFIP2.GFP
FEATURES             Location/Qualifiers
     source          1..9894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    914..938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4707..4731)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4742..4744,4745..4747,4748..5461)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4775..4803)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5398..5418
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(5483..5500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5489..5713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5759..6187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5846..5865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6056..6077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6196..6216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6201..6530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6381..6516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6443..6462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6597..7391
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6651..6670)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7261..7280
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7567..7688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7604..7623)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7658..7677
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7737..7753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7737..7753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7750..7772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7761..7777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7785..7791,7792..7809,7810..7815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7830..7851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7968..7985)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8139..8727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8219..8238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8898..9689,9690..9758))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9521..9540
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9759..9863)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttgat caaacaagtt tgtacaaaaa agcaggctcc accatgacca cgcacgtcac
      961 cctggaagat gccctgtcca acgtggacct gcttgaagag cttcccctcc ccgaccagca
     1021 gccatgcatc gagcctccac cttcctccat catgtaccag gctaactttg acacaaactt
     1081 tgaggacagg aatgcatttg tcacgggcat tgcaaggtac attgagcagg ctacagtcca
     1141 ctccagcatg aatgagatgc tggaggaagg acatgagtat gcggtcatgc tgtacacctg
     1201 gcgcagctgt tcccgggcca ttccccaggt gaaatgcaac gagcagccca accgagtaga
     1261 gatctatgag aagacagtag aggtgctgga gccggaggtc accaagctca tgaagttcat
     1321 gtattttcag cgcaaggcca tcgagcggtt ctgcagcgag gtgaagcggc tgtgccatgc
     1381 cgagcgcagg aaggactttg tctctgaggc ctacctcctg acccttggca agttcatcaa
     1441 catgtttgct gtcctggatg agctaaagaa catgaagtgc agcgtcaaga atgaccactc
     1501 tgcctacaag agggcagcac agttcctgcg gaagatggca gatccccagt ctatccagga
     1561 gtcgcagaac ctttccatgt tcctggccaa ccacaacagg atcacccagt gtctccacca
     1621 gcaacttgaa gtgatcccag gctatgagga gctgctggct gacattgtca acatctgtgt
     1681 ggattactac gagaacaaga tgtacctgac tcccagtgag aaacatatgc tcctcaaggt
     1741 gatgggcttt ggcctctacc taatggatgg aaatgtcagt aacatttaca aactggatgc
     1801 caagaagaga attaatctta gcaaaattga taaattcttt aagcagctgc aggtggtgcc
     1861 ccttttcggc gacatgcaga tagagctggc cagatacatt aagaccagtg ctcactatga
     1921 agagaacaag tccaagtgga cgtgcaccca gagcagcatc agcccccagt acaatatctg
     1981 cgagcagatg gttcagatcc gggatgacca catccgcttc atctccgagc tcgctcgcta
     2041 cagcaacagt gaggtggtga cgggctcagg gctggacagc cagaagtcag acgaggagta
     2101 tcgcgagctc ttcgacctag ccctgcgggg tctgcagctt ctatccaagt ggagcgccca
     2161 cgtcatggag gtgtactctt ggaagctggt tcatcccaca gacaagttct gcaacaagga
     2221 ctgtcctggc accgcggagg aatatgagag agccacacgc tacaattaca ccagtgagga
     2281 aaaatttgcc ttcgttgagg tgatcgccat gatcaaaggc ctgcaggtgc tcatgggcag
     2341 gatggagagc gtcttcaacc aggccatcag gaacaccatc tacgcggcat tgcaggactt
     2401 cgcccaggtg acgctgcgtg agcccctgcg gcaggcggta cggaagaaga agaatgtcct
     2461 catcagcgtc ctacaggcaa ttcgaaagac catctgtgac tgggagggag ggcgagagcc
     2521 ccctaatgac ccatgcttga gaggggagaa ggaccccaaa ggtggatttg atatcaaggt
     2581 gccccggcgt gctgtggggc catccagcac acagctgtac atggtgcgga ccatgcttga
     2641 atcactcatt gcagacaaaa gcggctccaa gaagaccctg aggagcagcc tggatggacc
     2701 cattgtcctc gccatagagg actttcacaa acagtccttc ttcttcacac atctgctcaa
     2761 catcagtgaa gccctgcagc agtgttgtga cctctcccag ctctggttcc gagaattctt
     2821 cctggagtta accatgggcc gacgaatcca gttccccatc gagatgtcca tgccctggat
     2881 tctaacggac catatcctgg aaaccaaaga accttccatg atggagtatg tcctctaccc
     2941 tctggatctg tacaacgaca gcgcctacta tgctctgacc aagtttaaaa agcagttcct
     3001 gtacgatgag atagaagctg aggtgaacct gtgttttgat cagtttgtct acaagctggc
     3061 agaccagatc tttgcttact acaaagccat ggctggcagt gtcctgttgg ataaacgttt
     3121 tcgagctgag tgtaagaatt atggcgtcat cattccgtat ccaccgtcca atcgctatga
     3181 aacactgctg aagcagagac acgtccagct gttgggtaga tcaattgact tgaacagact
     3241 cattacccag cgcacctctg ccgccatgta taaatccttg gaccaagcta tcagccgctt
     3301 tgagagtgag gacctgacct ccattgtgga gctggagtgg ctgctggaga ttaaccggct
     3361 cacgcatcgg ctgctctgta agcatatgac gctggacagc ttcgatgcca tgttccgaga
     3421 ggccaatcac aatgtgtccg ccccctatgg ccgtatcacc ctgcatgtct tctgggaact
     3481 gaactttgac tttctcccca actactgcta caatgggtcc actaaccgtt ttgtgcggac
     3541 tgccattcct ttcacccaag aaccacaacg agacaaacct gccaacgtcc agccttatta
     3601 cctctatgga tccaagcctc tcaacattgc ctacagccac atctacagct cctacaggaa
     3661 tttcgtgggg ccacctcatt tcaagactat ctgcagactc ctgggttatc agggcatcgc
     3721 tgtggtcatg gaggaactgc taaagattgt gaagagcttg ctccaaggaa ccattctcca
     3781 gtatgtgaaa acactgatag aggtgatgcc caagatatgc cgcttgcccc gacatgagta
     3841 tggctcccca gggatcctgg agttcttcca ccaccagctg aaggacatca ttgagtacgc
     3901 agagctcaaa acagacgtgt tccagagcct gagggaagtg ggcaatgcca tcctcttctg
     3961 cctcctcata gagcaagctc tgtctcagga ggaggtctgc gatttgctcc atgccgcacc
     4021 cttccaaaac atcttgccta gagtctacat caaagagggg gagcgcctgg aggtccggat
     4081 gaaacgtctg gaagccaagt atgccccgct ccacctggtc cctctgatcg agcggctggg
     4141 gacccctcag caaatcgcca ttgctcgcga gggtgacctc ctgaccaagg agcggctgtg
     4201 ctgtggcctg tccatgttcg aggtcatcct gacccgcatt cggagctacc tgcaggaccc
     4261 catctggcgg ggcccaccgc ccaccaatgg cgtcatgcac gtcgatgagt gtgtggagtt
     4321 ccaccggctg tggagcgcca tgcagttcgt gtactgcatc cctgtgggaa ccaacgagtt
     4381 cacagctgag cagtgtttcg gcgatggctt gaactgggct ggttgctcca tcattgtcct
     4441 gctgggccag cagcgtcgct ttgacctgtt cgacttctgt taccacctgc taaaagtgca
     4501 gaggcaggac gggaaggatg aaatcattaa gaatgtgccc ctgaagaaga tggccgaccg
     4561 gatcaggaag tatcagatct tgaacaatga ggtttttgcc atcctgaaca aatacatgaa
     4621 gtccgtggag acagacagtt ccactgtgga gcatgtgcgc tgcttccagc cacccatcca
     4681 ccagtccttg gccaccactt gcttggaccc agctttcttg tacaaagtgg ttcgatctag
     4741 aatggctagc aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     4801 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgctacata
     4861 cggaaagctt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     4921 acttgtcact actttctctt atggtgttca atgcttttcc cgttatccgg atcatatgaa
     4981 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc
     5041 tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct
     5101 tgttaatcgt atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca
     5161 caaactcgag tacaactata actcacacaa tgtatacatc acggcagaca aacaaaagaa
     5221 tggaatcaaa gctaacttca aaattcgcca caacattgaa gatggatccg ttcaactagc
     5281 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     5341 ttacctgtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg accacatggt
     5401 ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaata
     5461 atgaattaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5521 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5581 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5641 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5701 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     5761 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5821 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5881 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5941 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6001 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6061 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6121 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6181 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6241 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6301 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6361 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6421 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6481 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6541 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     6601 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     6661 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     6721 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     6781 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     6841 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     6901 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     6961 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7021 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7081 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7141 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7201 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7261 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7321 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     7381 agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg
     7441 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7501 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7561 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7621 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7681 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7801 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8041 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8101 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8521 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8581 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8641 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8701 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8761 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8821 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8881 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8941 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9001 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9061 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9121 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9181 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9241 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9301 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9361 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9421 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9481 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9541 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9601 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9661 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9721 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9781 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9841 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
