LOCUS       sequence-22850-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   McKenzie GJ, Craig NL
  TITLE     Fast, easy and efficient: site-specific insertion of transgenes into
            enterobacterial chromosomes using Tn7 without need for selection of 
            the insertion event.
  JOURNAL   BMC Microbiol. 2006 Apr 28;6:39. doi: 10.1186/1471-2180-6-39.
  PUBMED    16646962
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-04-28"; volume: "6:39. doi"; pages: " 
            10.1186/1471-2180-6-39"
COMMENT     Sequence Label: pGRG37
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    182..306
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(331..336,337..354,355..361)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(481..500)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgcaaatag atgggactgg gtgtagcgtc gtaagctaat acgaaaatta naaatgacac
       61 antngtttgg aactagattt cacttatctg gttggtcgac actagtatta ccctgttatc
      121 cctagattta aatgatatcg gatcctagta agccacgttt taattaatca gatcccatca
      181 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
      241 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      301 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
      361 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
      421 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      481 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
      541 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      601 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
      661 ggtgatatgg gatagtgctt cacccttgtt acaccgtttt ccatngagca aactgnaaac
      721 gtttttcatc gcc
//
