LOCUS       sequence-228503-       12566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 12566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL44 - pVND7>>
FEATURES             Location/Qualifiers
     source          1..12566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        complement(1850..2230)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             complement(join(2894..3604,3605..3607,3608..3610))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2936..2957)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3285..3304
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3544..3565
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3695..3741)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    3765..3784
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3817..3836
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3869..3888
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3921..3940
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             complement(4692..5036)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    10585..10609
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(10700..11288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10780..10799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11459..12247)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(12538..12566,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ggatctggct gagaacgcca gctgtgcatg cttggtctag
      601 aatacggtac ccctggattt tggttttagg aattagaaat tttattgata gaagtatttt
      661 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
      721 agaaccctaa ttcccttatc tgggaactac tcacacatta ttatagagag agatagattt
      781 gtagagagag actggtgatt tcagcgggca tgcctgcagg tcgacctgca gccaagctag
      841 gcatgatcta accctctgtc tctggcgtcg cgactgcgaa atttcgcgag ggtttccgag
      901 atggtgattg cgcttcgcag atctccaggc gcgtgggtgc ggacgtagtc agcgccattg
      961 ccgatcgcgt gaagttccgc cgcaaggctc gctggaccca gatcctttac aggaaggcca
     1021 acggtggcgc ccaagaagga tttccgcgac accgagacaa gtagcggaag ccccaacgcc
     1081 gacttcagct tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc ggggctcaag
     1141 aaaaatccca tccccggatc gaggatgagc cggtcggcag cgaccccgct ccgtcgcaag
     1201 gcggaaaccc gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc gggtcgaagg
     1261 tgaccggtgc gggtggcgat gccatcccgc tgcgctgagt gcataaccac cagcctgcag
     1321 tccgcctcag caatatcggg atagagcgca gggtcaggaa atccttggat atcgttcagg
     1381 tagcccacgc cgcgcttgag cgcatagcgc tgggtttccg gttggaagct gtcgattgaa
     1441 acacggtgca tctgatcgga cagggcgtct aagagcggcg caatacgtct gatctcatcg
     1501 gccggcgata caggcctcgc gtccggatgg ctggcggccg gtccgacatc cacgacgtct
     1561 gatccgactc gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc tagccgccgg
     1621 ctctcatcga agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt caccatgcac
     1681 tttactcttc caccattgct tgtaatggaa gtaatgtcag tgttgacctt cttaactggg
     1741 aatccagtca tggatttgag gccgccgaat ggagccaccg cggccgattg caccgtagaa
     1801 gcacggctga ctgtagtcac agctgaagag gatatcatag aagccatggc gatttggtgt
     1861 atcgagattg gttatgaaat tcagatgcta gtgtaatgta ttggtaattt gggaagatat
     1921 aataggaagc aaggctattt atccatttct gaaaaggcga aatggcgtca ccgcgagcgt
     1981 cacgcgcatt ccgttcttgc tgtaaagcgt tgtttggtac acttttgact agcgaggctt
     2041 ggcgtgtcag cgtatctatt caaaagtcgt taatggctgc ggatcaagaa aaagttggaa
     2101 tagaaacaga atacccgcga aattcaggcc cggttgccat gtcctacacg ccgaaataaa
     2161 cgaccaaatt agtagaaaaa taaaaactga ctcggatact tacgtcacgt cttgcgcact
     2221 gatttgaaaa ggatcctagt aacatacact ttcacttgtg atcatcaatt tacttgagag
     2281 tcgtttcttc aaagtttcaa actttccaca acagaaactc tacttaagat gatcaaggca
     2341 ttgtcaaatg aaatgctttg gaaggaagaa actgcctctc cttcgttaaa ccaagctcag
     2401 tatcttgcag tttcctctca tcagacatat caatgcttgg acaaaggtac gaaccatttt
     2461 ctacatttgt gggacattac aggcatttct tagcaacaac gaatgttcat catactcaga
     2521 acctaaaaga ttaacatgtt caagccatcc ttagaaccca acagctcaac actttcgcta
     2581 catctattac tagaggccaa caattggact atttacaagt ttcgcagaac tgcactaaac
     2641 agagtcgagt cactttgcag gcgtattaaa caaaagaagc acagataata aaaacgaagc
     2701 gatgataaag aagaagttcg acttgtcatt agaaagaaag agataacagg aacggaaaca
     2761 tagtagaaca cttattcatc agggattata caaggcccca aaacacaaac caccaaagtt
     2821 ttacatgaaa cgaaacattg aacttcttaa gcataacaga gacgagatgc agctaaagct
     2881 catcatgacc tgacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     2941 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     3001 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     3061 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
     3121 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgcta aagtagttgt
     3181 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     3241 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     3301 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     3361 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg
     3421 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     3481 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     3541 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     3601 tgctcaccat tgttgagcca ctagtggctg gattgggcag gagatagagg gaaagagcta
     3661 agaagagagc gagtgtgaag gctttcattg ttcgtcctct ccaaatgaaa tgaacttcct
     3721 tatatagagg aagggtcttg cgaagatcga tccactagtc tttcaattgt gagcgctcac
     3781 aattctttct cttccctttc ttctttctag tctttcaatt gtgagcgctc acaattcttt
     3841 ctcttccctt tcttctttct agtctttcaa ttgtgagcgc tcacaattct ttctcttccc
     3901 tttcttcttt ctagtctttc aattgtgagc gctcacaatt ctttctcttc cctttcttct
     3961 ttctagattt cttgagctcg acatatgcat aggttgtgac caggcatcat agcccctaca
     4021 gaacatcttg cctactcata gtccttctac gtggatcaga aggttttgac ttggacttct
     4081 taattgaaac ctcttactcg atgagagggt ttctcaatta aatttatttt ttaactcatt
     4141 ttttttattt attattatat gctcaatatt gatcatggtc aattttttaa aaaatatgaa
     4201 taaaaagttt tttgtgttta tgtttttttt tgtttttttt catcaccttc ttcacatgta
     4261 aacatttgta gtacatatta atacgtacat taattaatta tatacatggt gaacatatga
     4321 tttcgattca aatcgtgcat tttgggtttg agttacaaat ttgaggtttg atttgaaatt
     4381 ttgaaagtta ggagtctgat cagaaattac aagttctctt aactaatagg ccaccactca
     4441 aataaaaaat taatgggggt agaatgtatg gatgagttac aaatttggac aacagaataa
     4501 atgaatagaa aaacaaaggg actaattttt aaaaaaaaca aatccacctt tgtgaaattc
     4561 aacaacaaat ttctacgtaa taaacacaaa cttcttttac aaatagacag ttataaatgt
     4621 tattttccag atggaacaat taaatcatag aatacaaatc aatatataga agtgtgtttt
     4681 tttttaagca gttactcttt ttttgggttt ggtggggtat cttcatcatc gaatagatag
     4741 ttatatacat catccattgt agtggtatta aacatccctg tagtgattcc aaacgcgtta
     4801 tacgcagttt ggtccgtcca accaggtgac agtggttttg aattattacc atcatcaatt
     4861 ttactagccg tgatttcatt attcatgaag ttatcatgaa cgttagagga ggcaattggt
     4921 tgtgaaagcg cttgagaatt tgtttgagtt gttatgaggt tcggaccgtt gctactgtta
     4981 gtgaaagtga aggacaatga gctatcagca atattcccac tttgattaaa attggcgaat
     5041 tccgatccac tagtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     5101 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     5161 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     5221 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     5281 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     5341 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     5401 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     5461 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     5521 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     5581 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     5641 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     5701 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     5761 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     5821 agggccaagc tcgaggtcgc gacaccgatc agcaagcttt gtttaccagc cagctgctgc
     5881 gcaacacggt tcgggatgta gttgagctca gccatggcag cttcaacttt ttcccgggtt
     5941 ttagcgctaa catgtgaagc ctggttaacc actctagaaa cggtttgatg agaaacgccg
     6001 gcgtattcag cgacgtcgta taacgttacc ggtttgctag tagatttttg atgaaacaga
     6061 agctttttga tatttccatt tgaatatttt ggtatctgat tagtgatgat ttcagctaac
     6121 atctctggga attcaatact catggtctta tccaaaaatg tctggaagca gtaggtaagg
     6181 agattctcaa ccacctcatg catggagtcc agaagctttg tcagttggta aaaccgttgc
     6241 cagttctgac tggagttccc ttcccttttg acgatggctt ttcctagctc tttgatataa
     6301 gtcattcgaa tctcatcaaa taactcttgg ctcttcagac cttccttagg aactgaggag
     6361 agaagcagta aggttttcat acagagatac tcttcatagg atacctgcaa tctttgtaat
     6421 tcagaggaga caaacagcat gtgtttacat tggtcataca tgcagggtag agacattctc
     6481 tgctcattaa taatcagatc aggagcaaag cagagcaggt ttccacttga ttgtctgtat
     6541 gatctccaac ccagggcaaa tgccatgaga aacatccatg agtactgtag cagggtcatt
     6601 tggtcatcca ggtgtaagtt tctgaagcct ggtatcgcct ttgcccattt cactgctgca
     6661 atcacttgac gcccacctaa catgttgagt gtggtcataa ttctccatgc tgaatctgga
     6721 acagagctat catatcctgc atacaacacc tcgggttcaa tcacctccag cagtgacacc
     6781 aaggtagggg tgagctgtgg taatgctgca ggaactattg ttttgttagg attttccgaa
     6841 gtgtcttgtg agactcctgc agtggcttgc tgaatccctt tgattttttt ctttgttttt
     6901 cgagcttcac tagccattgt gttccacgat gatcctataa acgtatttaa tttggaaaat
     6961 tgttagagaa atgagtaaga aacaagatgg agaagtggca gagaagagaa tgatatgaag
     7021 gaaaatgaaa tgtatataaa cataagcgtg tctttttgga agctattgcg aaaaccttag
     7081 acaaacaagg cacaacctta aagttttgta tttgggacca aaaccaaagc ctattacaag
     7141 cttcgttata tttaattata agaaagggat cgaatggctc ttgggtccaa tgaaattcca
     7201 aaagctgtat gttgggtaat gatagtgatg ttacacacgg acgcacacga taatatacta
     7261 tatacctata aatatacaaa cacatatata tatatataca tgtaactaga tattatatcc
     7321 aaaagcttgg atctttaagc ttaaggctat cgctattata tgttgctgat gatggttatg
     7381 atgtattttt atgggaccac ccaacaatgg aggagctgtt actattttaa aagaaaaaat
     7441 gcaaaagctt gatagagaaa gtttttggac cagaaaaaaa tattaggttt aagggtttgt
     7501 ttcgccttct acataccgaa cgatgcttat aaatgaaaag attgaaactt tgtataatat
     7561 gtactatata taatgacata aggtagtatt ataagaccaa aaatatacaa gcagtactgt
     7621 atatatctgt aactatatgt aaaataatcg tatataaagg ctatgtagcc gaaaagcttg
     7681 acgggtggaa gtcactgagg ttggagtgtg ttacacactc tttgtcttga gttattttcc
     7741 tagactatct atataatata aattatatta tggtgcaaca acaattaatt tccaagaaac
     7801 aataatatct tagaaacaca catagattcc gaaatcccca ccatgattga tttcaattcc
     7861 ccagtttact ctaatatttg tgggtcaatc tcaacaggac cacacacatg tatactattc
     7921 aagatatgaa acgatacaaa acgatactat atgtaatatg tatgtatttt tgaataataa
     7981 aaacgtaaaa cacaaacgcc gatgtatcat catctggttc gtgcatgaag tgagtacagc
     8041 aacaaaacaa aaaaacccta aaaggagcaa tggggtgaaa cttacgagct aataaagatt
     8101 ggacgaaggg cttgttattt gcaagcgatt ttcacaagaa cgatctaaaa gagagtagca
     8161 aaaggaagaa ggagaggaag attgaaatct ctggtggtgt ggatcaaatg attgttaata
     8221 tagcaaagac aaggagagtt tggcttttga ttagatcaca tgcatatgga ccagctagaa
     8281 atcttagagt gtatatatag gtttatgcga gtatgcttaa ttatatgtat atggtaagtg
     8341 tgtgacatgt gggtgcaaaa tgatgatata agaagaagga gaagaagaag aagaaggaga
     8401 tgaaggaaag gtttgattgc gcgtgggata gacatctacg gtttgttttt ggtcggcgtc
     8461 tctctctctc tcttaccgca attttttgtt taaagttttt ccaatcttct ccactttacc
     8521 ggcagttact gcacaagcca aacctttttt ttatttgtct attgctcatt agttattatt
     8581 acatcacaca agacaaatcc tttagattaa cggtttttat gtggcgtaat taggtcaaat
     8641 ggatgttccg aatatatcca aaaacttcag tttgattgga taatatttca taacacgatt
     8701 ccatgtatta tcaataaaat cagatgacaa aaaaattacg ttttgaatat aattaatcta
     8761 ttttcttaaa acactatgtt cagctacaca ccataaattc ttataccatg taagatattt
     8821 tcagtaataa aaactagtta taaattgtga gtggcttgtt ggaaattcac aattcaagga
     8881 ttaaaaagga ctggatttgt aaacccgaag aagaactaca gaaatatatt cctatatgtt
     8941 tgctggatca ggtactgatg aaatgtgtct tagttttagt atgtcttatt aacccataga
     9001 taagacacgt aatacctcaa agttgttgag atattactat taactttttt gtcaaagaga
     9061 tattactatt aacttattaa gtatataaac atctcaacgt cgaaaggatt tacacgcatg
     9121 tacatttatg gattactacg taaatctcac tcaatatacc accgcagcta attttataat
     9181 gtgagtctag ctagatccac tcgaaaccac aatctcaact ttttcttaaa tgattaaact
     9241 aaactcagat ttatagtaaa cctaaaggaa cgaacattgg ccattaaaac atcaggaatc
     9301 gtgtatatat gaacattata ttatgaaaga aaaaataagg agaagagtaa aacacaggaa
     9361 tatgtttcat atgttcttaa gtctttctcc agcaatccag ctcccattgt ctggtttggt
     9421 ggcgccgtcc ttaacacgtg tcattacgaa tgtaagacac gtaagattca gacctgtttc
     9481 tatcacataa ctaatcattt gttcctcatc tgatttatcc gcttgcacgg gtccacccaa
     9541 agccgttggc tgtcgtttct tttacctcat cacctttcgg cttaaggtga cgtcatctca
     9601 gtaactcttt gaccctagtc caaagaaaaa tccaaaatac ttaacatatg acaaggtaga
     9661 cacagaattc gaggagaatg gatatataaa acagaaaaaa tgaaaattat aacaaatatt
     9721 gggtaccaaa aatagaaaag gcaaaagagg tagcacttga tttggagtca gattcacatg
     9781 catgcctatt tgtctactgc gcgagggaga gagagccttt tgttcaaatc ccaagaccat
     9841 ctatccatta accctttcac accattcact tttttcgtta tataaatgtc cctcgatccc
     9901 taattttcct ttctctaatc atagtgtctc taagtctcgt tatacacaca cacgtatata
     9961 acaatataac ccctaattct ttgcagtcga accatattta tggactcttt tatgtatggc
    10021 aatatggcat atacgagtta cgttcaccat gtagaatgac tacgcttttt ttctttttct
    10081 ttcagtttcg gtttagtttg atactactaa ttttggatat atcatgaagc accaaagcat
    10141 atctaagcat gccttttgtt tctccaaaaa gataaaatga tttcatatat atatttttat
    10201 taaaagaatt ggctatatat gttacgtata gattgatttt actaagtcgt gtcatgagat
    10261 agcaaatcat gtgtttttct tatttacaga ttatcgaagc tttttgaact atacttttat
    10321 cagttttatt atctatacat atgatgcatt gaactacata tatggatggt cacaactcac
    10381 aaatcatagt gcgtgtgtac ttgccactct ccactaatta taattagcgg taactgtaat
    10441 tagtgggttt agcaatttaa tttttgactt ttttcactca gcatacgtca acgtgctaag
    10501 gtggtacctg gtgcgatcgc tgttggcgcg ccgtgtttaa ttaaggttgc ggccgcttac
    10561 ttcgtccgag cctagttcga gccttgacag gatatattgg cgggtaaact aagtcgctgt
    10621 atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    11161 ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc ggcaaacaaa
    11221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11401 attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg tctagtgatt
    11461 atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct tccaactgat
    11521 ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct tcaagtatga
    11581 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
    11641 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
    11701 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
    11761 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
    11821 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
    11881 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
    11941 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
    12001 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
    12061 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
    12121 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
    12181 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
    12241 ccctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
    12301 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt
    12361 gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag
    12421 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc
    12481 tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag gcgatcccca
    12541 tccaacagcc cgccgtcgag cgggct
//
