LOCUS       sequence-228505-       11287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK645
FEATURES             Location/Qualifiers
     source          1..11287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      4497..4506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4503..5234
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             5310..5639
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    5789..5870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5892..6402
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             6439..7458
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6439..7458
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(7768..7785)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7774..7998
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9099..9119)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9099..9117)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9138..9154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9138..9154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9151..9173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9162..9178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9186..9192,9193..9210,9211..9216))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9231..9252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9540..10128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9620..9639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10299..11090,11091..11159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10922..10941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11160..11264)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgtt ccaggtggca actgatgctt
     3241 taaagagccg tgagccccag gctaagcccc agctggactt gagtattgac agcctggatc
     3301 tgagctgcga ggaggggacc ccactcagta tcaccagcaa gctgcctcgt acccagccag
     3361 acggcaccag cgtccctgga gaaccagcct cacctatctc ccagcgcctg ccccccaagg
     3421 tagaatccct ggagagtctc tacttcactc ccatccctgc tcggagtcag gcccccctgg
     3481 agagcagcct ggactccctg ggagacgtct tcctggactc gggtcgtaag acccgctccg
     3541 ctcgtcggcg caccacgcag atcatcaaca tcaccatgac caagaagcta gatgtggaag
     3601 agccagacag cgccaactca tcgttctaca gcacgcggtc tgctcctgct tcccaggcta
     3661 gcctgcgagc cacctcctct actcagtctc tagctcgcct gggttctccc gattatggca
     3721 actcagccct gctcagcttg cctggctacc gccccaccac tcgcagttct gctcgtcgtt
     3781 cccaggccgg ggtgtccagt ggggcccctc caggaaggaa cagcttctac atgggcactt
     3841 gccaggatga gcctgagcag ctggatgact ggaaccgcat tgcagagctg cagcagcgca
     3901 atcgagtgtg ccccccacat ctgaagacct gctatcccct ggagtccagg ccttccctga
     3961 gcctgggcac catcacagat gaggagatga aaactggaga cccccaagag accctgcgcc
     4021 gagccagcat gcagccaatc cagatagccg agggcactgg catcaccacc cggcagcagc
     4081 gcaaacgggt ctccctagag ccccaccagg gccctggaac tcctgagtct aagaaggcca
     4141 ccagctgttt cccacgcccc atgactcccc gagaccgaca tgaagggcgc aaacagagca
     4201 ctactgaggc ccagaagaaa gcagctccag cttctactaa acaggctgac cggcgccagt
     4261 cgatggcctt cagcatcctc aacacaccca agaagctagg gaacagcctt ctgcggcggg
     4321 gagcctcaaa gaaggccctg tccaaggctt cccccaacac tcgcagtgga acccgccgtt
     4381 ctccgcgcat tgccaccacc acagccagtg ccgccactgc tgccgccatt ggtgccaccc
     4441 ctcgagccaa gggcaaggca aagcacggag gtggatctac gcgtggaggt ggatctgcca
     4501 ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
     4561 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
     4621 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     4681 acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca
     4741 tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     4801 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     4861 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga
     4921 aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctggaag
     4981 gcagaaccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga
     5041 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg
     5101 accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg
     5161 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt aatggcatgg
     5221 acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaacgaa ttcggcattg
     5281 atctgagcgg cctgaccctg caggaattca gctccccgaa acgccctaag ctgctgcgtg
     5341 aatattacga ttggctggtt gataacagct ttaccccata tctggtggtg gatgccacat
     5401 acctgggcgt gaacgtgccc gtggagtatg tgaaagacgg tcagatcgtg ctgaatctgt
     5461 ctgcaagtgc gaccggcaac ctgcaactga caaatgattt tatccagttc aacgcccgct
     5521 ttaagggcgt gtctcgtgaa ctgtatatcc cgatgggtgc cgctctggcc atttacgctc
     5581 gcgagaacgg cgatggtgtg atgttcgaac cagaagaaat ctatgacgag ctgaatattg
     5641 gttaaaccgg tggatccaga tctctgcagc atatgtctag aggatcataa tcagccatac
     5701 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     5761 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     5821 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc cgacgtcagg
     5881 tggcactttt cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga
     5941 cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca
     6001 ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg
     6061 gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg
     6121 acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga
     6181 gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc
     6241 gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg
     6301 gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca
     6361 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggagctt
     6421 accatggata gatccggaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
     6481 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
     6541 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
     6601 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
     6661 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
     6721 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
     6781 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     6841 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     6901 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     6961 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     7021 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     7081 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     7141 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     7201 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     7261 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     7321 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     7381 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     7441 ccgagggcaa aggaatagag tagatgccga ccgaacaaga gctgatttcg agaacgcctc
     7501 agccagcaac tcgcgcgagc ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg
     7561 gcacagttct cgtccacagt tcgctaagct cgctcggctg ggtcgcggga gggccggtcg
     7621 cagtgattca ggcccttctg gattgtgttg gtccccaggg cacgattgtc atgcccacgc
     7681 actcgggtga tctgactgat cccgcagatt ggagatcgcc gcccgtgcct gccgattggg
     7741 tgcagatctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7801 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7861 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7921 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7981 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg atgagggagc
     8041 tgcggtggag gaggtggaga gaaggccgca cccttctggg cagggggagg ggagtgccgc
     8101 aataccttta tgggagttct ctgctgcctc ccgtcttgta aggaccgccc tgggcctgga
     8161 agaagccctc cctcctttcc tcctcgcgtg atctcgtcat cgcctccatg tcgagtcgct
     8221 tctcgattat gggcgggatt cttttgccta ggcttaaggg gctaacttgg tccctgggcg
     8281 ttgccctgca ggggagtgag cagctgtaag atttgagggg cgactccgat tagtttatct
     8341 tcccacggac tagagttggt gtcgaggtta ttgtaataag ggtggggtag ggaaatggag
     8401 cttagtcatt cacctggggc tgattttatg caacgagact gcggattatc actaatcatt
     8461 tttggagcat ttttctagag acagacataa agcatgatca cctgagtttt ataccatttg
     8521 agacccttgc tgcaccacca aagtgtagca tcaggttaaa tcttaataga aaaattttag
     8581 cttttgcttg agaaaccagt gcttccctcc ctcaccctct ctccccaggc tctctacccc
     8641 tttgcatccc taccaggcat cttagcaact ctcactcata cttgatccca ttttccattt
     8701 gttgtacttg ctcctccagt attcagacat agcactagct ttctccctct cttgatcttg
     8761 ggtagcctgg tgtctcgcga aaccagacag attggttcca ccacaaatta aggcttgagc
     8821 tggggcttga ctcttaccca gcagtgcttt tattcctccc tagttcacgt tcttaaatgt
     8881 ttatcttgat tttcatttta tcctttttcc ttagctggga ttctgtccct gaccgtcttc
     8941 acagtccagg tgatcttgac tactgcttta cagagaattg gatctgaggt taggcaacat
     9001 ctcccttttt cttcctctaa atacctctca tttctgttct taccagttag taactgatct
     9061 cagatgcctg tgtgatagct ggtacccagc ttttgttccc tttagtgagg gttaattgcg
     9121 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9181 ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9241 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9301 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9961 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10201 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10261 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10321 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10381 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10441 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    10501 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    10561 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    10621 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    10681 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10741 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10801 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10861 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10921 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10981 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11041 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11101 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11161 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11221 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11281 gtgccac
//
