LOCUS       sequence-228524-        5068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR donor plasmid to tag human transcription factor ZNF398 with 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5068)
  TITLE     White lab ENCODE plasmids to tag human transcription factors with 
            GFP
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pZNF398-donor
FEATURES             Location/Qualifiers
     source          1..5068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     520..539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1674..1694
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1707..1751
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             1791..1814
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(1842..1844,1845..1847,1848..2561)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1887..1908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2148..2167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    join(2598..3171,3172..3174,3175..3183)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2764..2781)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2991..3010
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3265..4068
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3328..3347)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3938..3957
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
       61 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
      121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
      181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggtgga cgtccagcca
      661 ctgacctgcc tgaagcctct gagggacaag tgacttttac tcagttgggt agctatcccc
      721 tcccacctcc agttggcgag caggtgttct catgccacca ctgtggcaag aatctcagcc
      781 aagacatgtt gctgacccac caatgtagcc atgctactga gcacccctta ccctgtgccc
      841 agtgccctaa gcactttact ccacaggcgg acctcagcag cacctcccag gaccatgcca
      901 gcgagacacc ccccacctgc ccacactgtg ccaggacttt tactcaccca tcaagactta
      961 cctaccatct tcgggtccat aacagcactg agcgtccttt cccctgtcct gattgcccca
     1021 agcgctttgc tgaccaggct cgactcacca gccaccggag agctcatgca agcgaaaggc
     1081 ccttccgctg tgcccagtgc ggcaggagct tcagcttgaa aatcagcctc ctgctccacc
     1141 agcggggtca tgcacaagag cgccctttct cctgccctca gtgtggcatt gacttcaacg
     1201 gccactcggc cctgatccgc caccagatga tccacacagg cgagcgtcct tacccctgca
     1261 ctgactgcag taagagcttc atgcgcaagg agcacctgct gaaccaccgg cggctgcaca
     1321 caggcgagcg gcccttcagt tgtcctcact gtggcaagag cttcatccgc aagcaccacc
     1381 taatgaaaca ccagcgcatc cacaccgggg agcggcccta cccctgctcc tactgtggca
     1441 ggagcttccg ctacaaacag acactcaagg accacctccg ttcaggccac aatggaggct
     1501 gtgggggtga tagtgaccca tcaggtcagc cacccaaccc accaggtccc ctcataactg
     1561 ggcttgaaac ttctggcctg ggtgtcaaca ctgaaggtct agagaccaac cagtggtatg
     1621 gggaagggag tggaggggga gttttggatt atgatattcc aactactgca agcgagaatc
     1681 tttattttca gggcgccgcc aaattcaaag aaaccgctgc tgctaaattc gaacgccagc
     1741 acatggacag cggaggtgga ggttcgagcg gtccctcggg ttcgtcgagc ctggaagttc
     1801 tgttccaggg gcccctgtcg tcgagcggtc cctcgggttc gatggtgagc aaaggcgagg
     1861 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1921 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1981 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2041 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2101 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2161 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2221 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2281 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2341 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2401 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2461 ccctgagcaa agaccccaac gagaagcgcg atcacatggt ccttaaggag ttcgtgaccg
     2521 ccgccgggat cactctcggc atggacgagc tgtacaagta atgatctaga gtcgagttaa
     2581 ttaagaattc cgcccccccc ctctccctcc ccccccctaa cgttactggc cgaagccgct
     2641 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     2701 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     2761 cccctctcgc caaaggaatg cagggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     2821 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     2881 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     2941 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     3001 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     3061 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     3121 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     3181 acctgttaca ttgcacaaga taaaaatata tcatcacgaa cagtaaaact gtctgcttac
     3241 ataaacagta atacaagggg tgttatggga tcggccattg aacaagatgg attgcacgca
     3301 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3361 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3421 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     3481 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3541 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3601 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     3661 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     3721 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     3781 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     3841 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3901 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3961 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4021 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgata aatccaaatc
     4081 tctgtggctt catgcttgta tatgctcaca gcagggcaca aaatccaaga gaaggtctgt
     4141 gagccccatc caacacccac agtaattatt atctggcaca tcaatgaatt tggggtccta
     4201 tacacttgac tagagacatg cttgtgtttt ggaatttcac acccttacaa gaataccaca
     4261 ttttgaaacc cagaaagacc tggaaaggag cccagcatgt ccatcttttc aaagatacaa
     4321 caaaagcaga agttacaaga atattgcgga gaggttggaa agcaggattt aacctctagt
     4381 tctcttgttc tcagagaaca gcaggttgat agtagattat gtctctaggt agagacagat
     4441 acatgagaag agcctccaaa actggaagcc catcataagg catgagaatg tttaactata
     4501 aagcctttta ttagttcttt gatagctaag atggttgttg gagaaagtgc ttaagccact
     4561 gtttatgttg ctgcctcctc tctcaaaacc agccaagatc tgttcacaca cctctgtggc
     4621 tgtctttccc tgaaacatgg taactcaaga ctgcctgcct aggtttctta gattaattat
     4681 tcacataaat tttgtttaat attggttgaa caagaaatct gactttaaaa gattattaag
     4741 gagaatattc cttaactata ctggcttgat ttgaaacagg ctcttattgc catttaaact
     4801 gccagaaatc tttgccaagg tcctggttaa aaaaattgga aacggttttc ttagccccag
     4861 ctttaaaagc tagagatctc agagaaatag ctggcttaac aatgacagaa cagcttaaaa
     4921 tttgtgccta caccatgttc tgagagtcgt tcacatcaaa ttcttatctt ttccaagcca
     4981 gtaagttggc ttctccattc ctggtgtctg atattcttga caatcatcag caacaaagat
     5041 cacagcactt tccagaagca tggagctt
//
