LOCUS       sequence-228552-        6523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Tp901-Int5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2009 to 2043)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-8
FEATURES             Location/Qualifiers
     source          1..6523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2009..2043
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             3766..3777
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4219..4236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4526..4545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5141..5235)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(5333..5401,5402..6193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6197..6299)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6476..6519)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtaccttaa tgtgtggatc acacaggaaa gtactagatg actaagaaag
      421 tagcaatcta tacacgagta tccactacta accaagcaga ggaaggcttc tcaattgatg
      481 agcaaattga ccgtttaaca aaatatgctg aagcaatggg gtggcaagta tctgatactt
      541 atactgatgc tggtttttca ggggccaaac ttgaacgccc agcaatgcaa agattaatca
      601 acgatatcga gaataaagct tttgatacag ttcttgtata taagctagac cgcctttcac
      661 gtagtgtaag agatactctt tatcttgtta aggatgtgtt cacaaaaaat aaaatagact
      721 ttatctcgct taatgaaagt attgatactt cttctgctat gggtagcttg tttctcacta
      781 ttctttctgc aattaatgag tttgaaagag agaatataaa agaacgcatg actatgggta
      841 aactagggcg agcgaaatct ggtaagtcta tgatgtggac taagacagct tttgggtatt
      901 accacaacag aaagacaggt atattagaaa ttgttccttt acaagctaca atagttgaac
      961 aaatattcac tgattattta tcaggaatat cacttacaaa attaagagat aaactcaatg
     1021 aatctggaca catcggtaaa gatataccgt ggtcttatcg taccctaaga caaacacttg
     1081 ataatccagt ttactgtggt tatatcaaat ttaaggacag cctatttgaa ggtatgcaca
     1141 aaccaattat cccttatgag acttatttaa aagttcaaaa agagctagaa gaaagacaac
     1201 agcagactta tgaaagaaat aacaacccta gacctttcca agctaaatat atgctgtcag
     1261 ggatggcaag gtgcggttac tgtggagcac ctttaaaaat tgttcttggc cacaaaagaa
     1321 aagatggaag ccgcactatg aaatatcact gtgcaaatag atttcctcga aaaacaaaag
     1381 gaattacagt atataatgac aataaaaagt gtgattcagg aacttatgat ttaagtaatt
     1441 tagaaaatac tgttattgac aacctgattg gatttcaaga aaataatgac tccttattga
     1501 aaattatcaa tggcaacaac caacctattc ttgatacttc gtcatttaaa aagcaaattt
     1561 cacagatcga taaaaaaata caaaagaact ctgatttgta cctaaatgat tttatcacta
     1621 tggatgagtt gaaagatcgt actgattccc ttcaggctga gaaaaagctg cttaaagcta
     1681 agattagcga aaataaattt aatgactcta ctgatgtttt tgagttagtt aaaactcagt
     1741 tgggctcaat tccgattaat gaactatcat atgataataa aaagaaaatc gtcaacaacc
     1801 ttgtatcaaa ggttgatgtt actgctgata atgtagatat catatttaaa ttccaactcg
     1861 ctaccggtta aattgaacct ctactacgag tgagttgaga ttacagcctt actcggtacc
     1921 aaattccaga aaagagacgc tttcgagcgt cttttttcgt tttggtccct tacgctatta
     1981 ttgaagccag tctgttaccg aagtgaaatt gacggctagc tcagtcctag gtacagtgct
     2041 agccagagga aggaggctcg atgcctggta tgaccaccga aaccggtccg gatccggcag
     2101 gtctgattga cctgttttgt cgtaaaagca aagcagttaa aagccgtgca aatggtgcag
     2161 gtcagcgtcg taaacaagaa attagcattg cagcacaaga aaccctgggt cgtaaagttg
     2221 cagcactgct gggtatgcag gttcgtcatg tttggaaaga agttggtagc gcaagccgtt
     2281 ttcgtaaagg taaagcacgt gatgatcaga gcaaagcact gaaagccctg gaaagcggtg
     2341 aagttggtgc actgtggtgt tatcgtctgg atcgttggga tcgtggtggt gccggtgcaa
     2401 ttctgaaaat tatcgaaccg gaagatggta tgcctcgtcg tctgctgttt ggttgggatg
     2461 aagataccgg tagtccggtt ctggatagca ccaataaacg tgatcgcggt gaactgattc
     2521 gtcgtgcaga agaagcacgc gaagaagcag aaaaactgag cgaacgtgtt cgtgatacca
     2581 aagcacatca gcgtgaaaat ggtgaatggg ttaatgcccg tgcaccgtat ggtctgcgtg
     2641 ttgttctggt taccgttagt gatgaagagg gtgatgaata tgatgaacgt aaactggcag
     2701 cagatgatga agatgcgggt ggtcctgatg gtctgaccaa agcagaagca gcccgtctgg
     2761 tttttaccct gccggttacc gatcgtctga gctatgcagg caccgcacat gcaatgaata
     2821 cccgtgaaat tccgagcccg accggtggtc cgtggattgc agttaccgtg cgtgatatga
     2881 ttcagaatcc ggcatatgcg ggttggcaga ccacaggtcg tcaggatggt aaacagcgtc
     2941 gtctgacctt ttataacggt gaaggtaaac gtgttagcgt tatgcatggt cctccgctgg
     3001 tgaccgatga agaacaagaa gccgcaaaag cagccgttaa aggtgaagat ggtgttggtg
     3061 ttccgctgga tggtagcgat catgataccc gtcgcaaaca tctgctgagc ggtcgtatgc
     3121 gttgtccggg ttgtggtggt agctgtagct atagcggtaa tggttatcgt tgttggcgta
     3181 gcagtgtgaa aggtggttgt ccggcaccga cctatgttgc acgtaaaagc gttgaagaat
     3241 atgttgcatt tcgttgggca gcaaaattag cagcaagcga accggatgat ccgtttgtta
     3301 ttgcagttgc agatcgctgg gcagcactga cccatccgca ggcaagcgaa gatgaaaagt
     3361 atgcaaaagc cgcagttcgt gaagccgaaa aaaatctggg tcgcctgctg cgtgatcgtc
     3421 agaatggtgt ttatgatggt ccggcagaac agttttttgc ccctgcatat caagaagcac
     3481 tgagcacctt gcaggcagcc aaagatgcag ttagcgaaag cagcgcaagc gcagcagttg
     3541 atgttagctg gattgttgat agcagcgatt atgaagaact gtggctgcgt gcaaccccga
     3601 ccatgcgtaa tgcaattatt gatacctgca tcgatgaaat ttgggttgca aaaggccagc
     3661 gtggtcgtcc gtttgatggt gatgaacgcg ttaaaatcaa atgggcagcc cgtacctaaa
     3721 acgattacgg attgctctac tgtgactgaa gtttacaacc caattattga aggccgctaa
     3781 cgcggccttt ttttgtttct ggtctccctg aggcacagag atttacttta ttcacgactt
     3841 cagatacgca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt
     3901 gctggataac tttacgggca tgcataaggc tcgtatgata tattcaggga gaccacaacg
     3961 gtttccctct acaaataatt ttgtttaact ttagtaattt caacaaaata actaggattc
     4021 gaaatacggt ctactacaga agggtggttt atcttactca acgacgaaca ataaggcctc
     4081 cctaacgggg ggcctttttt attgataaca aaaagatttc cctcgtcacg cagtaagtat
     4141 ttatcgtaga agatcacttt tatcggtttc cagaacaggt aagagccaat agtgattact
     4201 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4261 agcggtatca gctcactcaa aggcggtaat cctagctagg gcgttcggct gcggcgagcg
     4321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4801 actggtaaca ggaagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4981 aacaaaccac cgctggtagc ggtggttttt tgtttgcaag cagcagatta cgcgcagaaa
     5041 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5101 aaactcacgt taagggattt tggtcatgac ttgtgcttgg attctcacca ataaaaaacg
     5161 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     5221 ctatcagcag gagtccaagc gagctcgata tcactaaata cattcaaata tctatccgct
     5281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagtat
     5341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5401 tcacccagaa acgctggtga aagtaaaaga tgccgaagat cagttgggtg cacgtgtggg
     5461 ttacatcgaa ctggacctca acagcggtaa gattcttgag agttttcgcc ccgaagaacg
     5521 tttcccaatg atgagcactt ttaaagttct gctctgtggc gcggtattat cccgtattga
     5581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5641 ctcaccagtc acagaaaagc atcttacgga cggcatgaca gtacgcgaat tatgcagcgc
     5701 tgccataacc atgagtgata acacggcggc caacttactt ctgacaacga tcggaggacc
     5761 gaaggagctt accgcttttt tgcacaacat gggtgatcat gtaactcgcc ttgatcgttg
     5821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5881 tatggcaaca acgttgcgca aactcttaac tggcgaactt cttactctcg cttcccggca
     5941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6001 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6061 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctccctgat
     6181 taagcattgg taataattta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     6241 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     6301 cgtctcattt tcgccagata tcgacgtcta agaaaccatt attatcatga cattaaccta
     6361 taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagtgccacc tgacgtctaa
     6421 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     6481 agataaaaaa aatccttagc tttcgctaag gatgatttct gga
//
