LOCUS       sequence-228553-        6253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Tp901-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2009 to 2043)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-9
FEATURES             Location/Qualifiers
     source          1..6253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2009..2043
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             2107..2118
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3949..3966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4256..4275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4871..4965)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(5063..5131,5132..5923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5927..6029)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6206..6249)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtaccttaa tgtgtggatc acacaggaaa gtactagatg actaagaaag
      421 tagcaatcta tacacgagta tccactacta accaagcaga ggaaggcttc tcaattgatg
      481 agcaaattga ccgtttaaca aaatatgctg aagcaatggg gtggcaagta tctgatactt
      541 atactgatgc tggtttttca ggggccaaac ttgaacgccc agcaatgcaa agattaatca
      601 acgatatcga gaataaagct tttgatacag ttcttgtata taagctagac cgcctttcac
      661 gtagtgtaag agatactctt tatcttgtta aggatgtgtt cacaaaaaat aaaatagact
      721 ttatctcgct taatgaaagt attgatactt cttctgctat gggtagcttg tttctcacta
      781 ttctttctgc aattaatgag tttgaaagag agaatataaa agaacgcatg actatgggta
      841 aactagggcg agcgaaatct ggtaagtcta tgatgtggac taagacagct tttgggtatt
      901 accacaacag aaagacaggt atattagaaa ttgttccttt acaagctaca atagttgaac
      961 aaatattcac tgattattta tcaggaatat cacttacaaa attaagagat aaactcaatg
     1021 aatctggaca catcggtaaa gatataccgt ggtcttatcg taccctaaga caaacacttg
     1081 ataatccagt ttactgtggt tatatcaaat ttaaggacag cctatttgaa ggtatgcaca
     1141 aaccaattat cccttatgag acttatttaa aagttcaaaa agagctagaa gaaagacaac
     1201 agcagactta tgaaagaaat aacaacccta gacctttcca agctaaatat atgctgtcag
     1261 ggatggcaag gtgcggttac tgtggagcac ctttaaaaat tgttcttggc cacaaaagaa
     1321 aagatggaag ccgcactatg aaatatcact gtgcaaatag atttcctcga aaaacaaaag
     1381 gaattacagt atataatgac aataaaaagt gtgattcagg aacttatgat ttaagtaatt
     1441 tagaaaatac tgttattgac aacctgattg gatttcaaga aaataatgac tccttattga
     1501 aaattatcaa tggcaacaac caacctattc ttgatacttc gtcatttaaa aagcaaattt
     1561 cacagatcga taaaaaaata caaaagaact ctgatttgta cctaaatgat tttatcacta
     1621 tggatgagtt gaaagatcgt actgattccc ttcaggctga gaaaaagctg cttaaagcta
     1681 agattagcga aaataaattt aatgactcta ctgatgtttt tgagttagtt aaaactcagt
     1741 tgggctcaat tccgattaat gaactatcat atgataataa aaagaaaatc gtcaacaacc
     1801 ttgtatcaaa ggttgatgtt actgctgata atgtagatat catatttaaa ttccaactcg
     1861 ctaccggtta aattgaacct ctactacgag tgagttgaga ttacagcctt actcggtacc
     1921 aaattccaga aaagagacgc tttcgagcgt cttttttcgt tttggtccct tacgctatta
     1981 ttgaagccag tctgttaccg aagtgaaatt gacggctagc tcagtcctag gtacagtgct
     2041 agccagagga aggaggctcg aacgattacg gattgctcta ctgtgactga agtttacaac
     2101 ccaattattg aaggccgcta acgcggcctt tttttgtttc tggtctccct gaggcacaga
     2161 gatttacttt attcacgact tcagatacgc acagctaaca ccacgtcgtc cctatctgct
     2221 gccctaggtc tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtatgat
     2281 atattcaggg agaccacaac ggtttccctc tacaaataat tttgtttaac tttagtaatt
     2341 tcaacaaaat aactaggatt cgaatgaaag tggccattta tgttcgtgtt agcaccgatg
     2401 aacaggccaa agaaggtttt agcattccgg cacagcgtga acgtctgcgt gcattttgtg
     2461 caagccaggg ttgggaaatt gtgcaagaat atattgaaga aggttggagc gcaaaagatc
     2521 tggatcgtcc gcagatgcag cgtctgctga aagatatcaa aaaaggcaac attgatattg
     2581 tgctggtgta tcgtctggat cgcctgaccc gtagcgttct ggatctgtat ctgctgttac
     2641 agacctttga aaaatacaat gtggcatttc gtagcgccac cgaagtttat gataccagca
     2701 ccgcaatggg tcgtctgttt attaccctgg ttgcagcact ggcacagtgg gaacgtgaaa
     2761 atctggcaga acgtgttaaa tttggtatcg agcagatgat cgatgaaggt aaaaaaccgg
     2821 gtggtcatag cccgtatggt tacaaatttg ataaagactt caattgcacc attattgagg
     2881 aagaagcaga cgttgttcgt atgatctatc gcatgtattg tgatggttat ggctatcgta
     2941 gcattgcaga tcgtctgaat gaactgatgg ttaaaccgcg tattgccaaa gaatggaatc
     3001 ataatagcgt gcgtgatatc ctgaccaacg atatctatat tggcacctat cgttggggtg
     3061 ataaagttgt tccgaataat catccgccta ttattagcga aaccctgttc aaaaaagccc
     3121 agaaagaaaa agaaaaacgt ggcgttgatc gtaaacgcgt tggtaaattt ctgtttaccg
     3181 gtctgttaca gtgtggtaat tgtggtggcc ataaaatgca gggccatttt gataaacgtg
     3241 agcagaaaac ctattaccgt tgtaccaaat gtcaccgcat taccaacgaa aaaaacattc
     3301 tggaaccgct gctggatgaa attcagctgc tgattaccag caaagaatac tttatgagca
     3361 aattcagcga ccgctatgat cagcaagagg ttgttgatgt tagcgcactg acaaaagaac
     3421 tggaaaaaat caaacgccag aaagagaaat ggtacgatct gtatatggat gatcgtaacc
     3481 cgattccgaa agaagaactg tttgccaaaa ttaacgaact gaacaaaaaa gaagaagaaa
     3541 tctatagcaa gctgagcgaa gtggaagaag ataaagaacc ggttgaagag aaatataacc
     3601 gcctgagcaa aatgatcgat tttaaacagc agtttgagca ggccaacgac tttaccaaaa
     3661 aagagctgct gttcagcatc ttcgaaaaga ttgtgattta tcgcgagaaa ggcaagctga
     3721 aaaaaatcac cctggattac accctgaaat aaaatacggt ctactacaga agggtggttt
     3781 atcttactca acgacgaaca ataaggcctc cctaacgggg ggcctttttt attgataaca
     3841 aaaagatttc cctcgtcacg cagtaagtat ttatcgtaga agatcacttt tatcggtttc
     3901 cagaacaggt aagagccaat agtgattact tcgcgttatg caggcttcct cgctcactga
     3961 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4021 cctagctagg gcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4501 agacacgact tatcgccact ggcagcrrct tatcgccact ggcagcagcc actggtaaca
     4561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4621 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac
     4861 ttgtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     4921 ccagatggag ttctgaggtc attactggat ctatcagcag gagtccaagc gagctcgata
     4981 tcactaaata cattcaaata tctatccgct catgagacaa taaccctgat aaatgcttca
     5041 ataatattga aaaaggaaga atatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5101 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5161 tgccgaagat cagttgggtg cacgtgtggg ttacatcgaa ctggacctca acagcggtaa
     5221 gattcttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct
     5281 gctctgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5341 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5401 cggcatgaca gtacgcgaat tatgcagcgc tgccataacc atgagtgata acacggcggc
     5461 caacttactt ctgacaacga tcggaggacc gaaggagctt accgcttttt tgcacaacat
     5521 gggtgatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5581 cgacgagcgt gacaccacga tgcctgtagc tatggcaaca acgttgcgca aactcttaac
     5641 tggcgaactt cttactctcg cttcccggca acaattaata gactggatgg aggcggataa
     5701 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5761 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5821 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5881 acagatcgct gagataggtg cctccctgat taagcattgg taataattta gcttccttag
     5941 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     6001 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccagata tcgacgtcta
     6061 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6121 tcttcacctc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     6181 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
     6241 gatgatttct gga
//
