LOCUS       sequence-228555-        6449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Int5-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 546 to 580)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-10
FEATURES             Location/Qualifiers
     source          1..6449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        546..580
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             2303..2314
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4145..4162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4452..4471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5067..5161)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(5259..5327,5328..6119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6123..6225)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6402..6445)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtacctata atgtgtggat cacacaggaa agtactagat tgaacctcta
      421 ctacgagtga gttgagatta cagccttact cggtaccaaa ttccagaaaa gagacgcttt
      481 cgagcgtctt ttttcgtttt ggtcccttac gctattattg aagccagtct gttaccgaag
      541 tgaaattgac ggctagctca gtcctaggta cagtgctagc cagaggaagg aggctcgatg
      601 cctggtatga ccaccgaaac cggtccggat ccggcaggtc tgattgacct gttttgtcgt
      661 aaaagcaaag cagttaaaag ccgtgcaaat ggtgcaggtc agcgtcgtaa acaagaaatt
      721 agcattgcag cacaagaaac cctgggtcgt aaagttgcag cactgctggg tatgcaggtt
      781 cgtcatgttt ggaaagaagt tggtagcgca agccgttttc gtaaaggtaa agcacgtgat
      841 gatcagagca aagcactgaa agccctggaa agcggtgaag ttggtgcact gtggtgttat
      901 cgtctggatc gttgggatcg tggtggtgcc ggtgcaattc tgaaaattat cgaaccggaa
      961 gatggtatgc ctcgtcgtct gctgtttggt tgggatgaag ataccggtag tccggttctg
     1021 gatagcacca ataaacgtga tcgcggtgaa ctgattcgtc gtgcagaaga agcacgcgaa
     1081 gaagcagaaa aactgagcga acgtgttcgt gataccaaag cacatcagcg tgaaaatggt
     1141 gaatgggtta atgcccgtgc accgtatggt ctgcgtgttg ttctggttac cgttagtgat
     1201 gaagagggtg atgaatatga tgaacgtaaa ctggcagcag atgatgaaga tgcgggtggt
     1261 cctgatggtc tgaccaaagc agaagcagcc cgtctggttt ttaccctgcc ggttaccgat
     1321 cgtctgagct atgcaggcac cgcacatgca atgaataccc gtgaaattcc gagcccgacc
     1381 ggtggtccgt ggattgcagt taccgtgcgt gatatgattc agaatccggc atatgcgggt
     1441 tggcagacca caggtcgtca ggatggtaaa cagcgtcgtc tgacctttta taacggtgaa
     1501 ggtaaacgtg ttagcgttat gcatggtcct ccgctggtga ccgatgaaga acaagaagcc
     1561 gcaaaagcag ccgttaaagg tgaagatggt gttggtgttc cgctggatgg tagcgatcat
     1621 gatacccgtc gcaaacatct gctgagcggt cgtatgcgtt gtccgggttg tggtggtagc
     1681 tgtagctata gcggtaatgg ttatcgttgt tggcgtagca gtgtgaaagg tggttgtccg
     1741 gcaccgacct atgttgcacg taaaagcgtt gaagaatatg ttgcatttcg ttgggcagca
     1801 aaattagcag caagcgaacc ggatgatccg tttgttattg cagttgcaga tcgctgggca
     1861 gcactgaccc atccgcaggc aagcgaagat gaaaagtatg caaaagccgc agttcgtgaa
     1921 gccgaaaaaa atctgggtcg cctgctgcgt gatcgtcaga atggtgttta tgatggtccg
     1981 gcagaacagt tttttgcccc tgcatatcaa gaagcactga gcaccttgca ggcagccaaa
     2041 gatgcagtta gcgaaagcag cgcaagcgca gcagttgatg ttagctggat tgttgatagc
     2101 agcgattatg aagaactgtg gctgcgtgca accccgacca tgcgtaatgc aattattgat
     2161 acctgcatcg atgaaatttg ggttgcaaaa ggccagcgtg gtcgtccgtt tgatggtgat
     2221 gaacgcgtta aaatcaaatg ggcagcccgt acctaaaacg attacggatt gctctactgt
     2281 gactgaagtt tacaacccaa ttattgaagg ccgctaacgc ggcctttttt tgtttctggt
     2341 ctccctgagg cacagagatt tactttattc acgacttcag atacgcacag ctaacaccac
     2401 gtcgtcccta tctgctgccc taggtctatg agtggttgct ggataacttt acgggcatgc
     2461 ataaggctcg tatgatatat tcagggagac cacaacggtt tccctctaca aataattttg
     2521 tttaacttta gtaatttcaa caaaataact aggattcgaa tgaaagtggc catttatgtt
     2581 cgtgttagca ccgatgaaca ggccaaagaa ggttttagca ttccggcaca gcgtgaacgt
     2641 ctgcgtgcat tttgtgcaag ccagggttgg gaaattgtgc aagaatatat tgaagaaggt
     2701 tggagcgcaa aagatctgga tcgtccgcag atgcagcgtc tgctgaaaga tatcaaaaaa
     2761 ggcaacattg atattgtgct ggtgtatcgt ctggatcgcc tgacccgtag cgttctggat
     2821 ctgtatctgc tgttacagac ctttgaaaaa tacaatgtgg catttcgtag cgccaccgaa
     2881 gtttatgata ccagcaccgc aatgggtcgt ctgtttatta ccctggttgc agcactggca
     2941 cagtgggaac gtgaaaatct ggcagaacgt gttaaatttg gtatcgagca gatgatcgat
     3001 gaaggtaaaa aaccgggtgg tcatagcccg tatggttaca aatttgataa agacttcaat
     3061 tgcaccatta ttgaggaaga agcagacgtt gttcgtatga tctatcgcat gtattgtgat
     3121 ggttatggct atcgtagcat tgcagatcgt ctgaatgaac tgatggttaa accgcgtatt
     3181 gccaaagaat ggaatcataa tagcgtgcgt gatatcctga ccaacgatat ctatattggc
     3241 acctatcgtt ggggtgataa agttgttccg aataatcatc cgcctattat tagcgaaacc
     3301 ctgttcaaaa aagcccagaa agaaaaagaa aaacgtggcg ttgatcgtaa acgcgttggt
     3361 aaatttctgt ttaccggtct gttacagtgt ggtaattgtg gtggccataa aatgcagggc
     3421 cattttgata aacgtgagca gaaaacctat taccgttgta ccaaatgtca ccgcattacc
     3481 aacgaaaaaa acattctgga accgctgctg gatgaaattc agctgctgat taccagcaaa
     3541 gaatacttta tgagcaaatt cagcgaccgc tatgatcagc aagaggttgt tgatgttagc
     3601 gcactgacaa aagaactgga aaaaatcaaa cgccagaaag agaaatggta cgatctgtat
     3661 atggatgatc gtaacccgat tccgaaagaa gaactgtttg ccaaaattaa cgaactgaac
     3721 aaaaaagaag aagaaatcta tagcaagctg agcgaagtgg aagaagataa agaaccggtt
     3781 gaagagaaat ataaccgcct gagcaaaatg atcgatttta aacagcagtt tgagcaggcc
     3841 aacgacttta ccaaaaaaga gctgctgttc agcatcttcg aaaagattgt gatttatcgc
     3901 gagaaaggca agctgaaaaa aatcaccctg gattacaccc tgaaataaaa tacggtctac
     3961 tacagaaggg tggtttatct tactcaacga cgaacaataa ggcctcccta acggggggcc
     4021 ttttttattg ataacaaaaa gatttccctc gtcacgcagt aagtatttat cgtagaagat
     4081 cacttttatc ggtttccaga acaggtaaga gccaatagtg attacttcgc gttatgcagg
     4141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4201 actcaaaggc ggtaatccta gctagggcgt tcggctgcgg cgagcggtat cagctcactc
     4261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggca
     4741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4801 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4921 ggtagcggtg gttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5041 ggattttggt catgacttgt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     5101 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat cagcaggagt
     5161 ccaagcgagc tcgatatcac taaatacatt caaatatcta tccgctcatg agacaataac
     5221 cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagtattcaa catttccgtg
     5281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5341 tggtgaaagt aaaagatgcc gaagatcagt tgggtgcacg tgtgggttac atcgaactgg
     5401 acctcaacag cggtaagatt cttgagagtt ttcgccccga agaacgtttc ccaatgatga
     5461 gcacttttaa agttctgctc tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5581 aaaagcatct tacggacggc atgacagtac gcgaattatg cagcgctgcc ataaccatga
     5641 gtgataacac ggcggccaac ttacttctga caacgatcgg aggaccgaag gagcttaccg
     5701 cttttttgca caacatgggt gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagctatg gcaacaacgt
     5821 tgcgcaaact cttaactggc gaacttctta ctctcgcttc ccggcaacaa ttaatagact
     5881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6061 tggatgaacg aaatagacag atcgctgaga taggtgcctc cctgattaag cattggtaat
     6121 aatttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     6181 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
     6241 cagatatcga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     6301 tcacgaggcc ctttcgtctt cacctcgagt gccacctgac gtctaagaaa ccattattat
     6361 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     6421 cttagctttc gctaaggatg atttctgga
//
