LOCUS       sequence-228557-        6150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   de Blas, Angel
  TITLE     GABAA receptor subunit plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Myc-gamma3 (in pCAGGS)
FEATURES             Location/Qualifiers
     source          1..6150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          198..1214
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1138..1160
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1222..1241
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1332..1361
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2706..2725)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2771..2826
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2825..2844)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3187..3203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3187..3203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3200..3222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3211..3227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3235..3241,3242..3259,3260..3265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3280..3301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3360..3555
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3406..3541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3561..3695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3598..3617)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3652..3671
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3763..3780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3934..4522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4014..4033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4693..5484,5485..5553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5316..5335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5554..5658)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5689..6068
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(6070..6150,1..197)
                     /label=chicken beta-actin promoter
ORIGIN
        1 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
       61 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
      121 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
      181 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
      241 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      301 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
      361 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
      421 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
      481 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
      541 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
      601 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
      661 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
      721 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
      781 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
      841 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
      901 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
      961 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     1021 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     1081 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     1141 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     1201 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     1261 aagaattcat ggctgcaaag ctgctgcttc tcctctgcct gttctcgggc ttgcatgctc
     1321 ggtccaggag ggaacagaag ttgatttccg aagaagacct cgtggaagag gatgacagtg
     1381 aagactctcc atcaaaccag aagtgggtct tggcacccaa atcacaagac accgatgtga
     1441 cactgattct caacaaattg ctgcgggaat atgacaaaaa gttgagacca gacattggaa
     1501 taaaaccaac tgtgatcgat gtggacattt atgttaacag cattggtcct gtgtcatcca
     1561 taaacatgga ataccaaatt gatatatttt ttgctcaaac ctggacagac agtcgccttc
     1621 gattcaacag cacaatgaaa atacttactc tgaacagcaa catggtgggt ttgatatgga
     1681 taccagacac catctttcga aactctaaaa cagcagaggc tcactggatc accacaccca
     1741 accagctcct tagaatctgg aatgatggga aaatccttta cacattaagg cttaccatca
     1801 atgcagagtg ccagctgcaa ctgcataact tccccatgga tgcgcatgcc tgccctttga
     1861 ccttctctag ctatggctac cccaaagaag aaatgattta tcgttggagg aaaaattcag
     1921 ttgaggcagc tgatcagaaa tcatggcggc tctatcagtt tgacttcatg ggcctcagaa
     1981 acactacaga aatcgtgact acatctgcag gtgattatgt tgtcatgact atctactttg
     2041 aactgagtcg aagaatggga tacttcacta tccagacgta tatcccctgc atactgactg
     2101 tggttctgtc ctgggtatca ttttggatca aaaaagatgc gacaccagca agaacaacat
     2161 taggcatcac cacggtgcta accatgacca cactcagcac cattgccagg aagtccctgc
     2221 ctcgggtgtc ctatgtcact gccatggacc tctttgtgac tgtgtgcttc ttgtttgtct
     2281 ttgccgcact gatggagtat gctactctca actactattc gagctgtcga aagccaacca
     2341 tcaggaagaa aaagacatcg ttattacatc cagattcaac aagatggatt cctgatcgaa
     2401 taagccttca agcaccctct aattattctc tcctggatat gagaccccca ccacccgtga
     2461 tgatcacgtt aaacaattcc atgtactggc aggagtttga ggacacctgt gtctatgagt
     2521 gtctggatgg caaagactgc cagagcttct tctgctgcta tgaggagtgc aagtctggct
     2581 cctggagaag aggccgcatc cacattgacg tctctgagct ggactcctac tctcgggtct
     2641 tcttcccaac gtccttcctg ctgttcaacc tggtctattg ggttggatac ctatatcttt
     2701 taagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     2761 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     2821 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     2881 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     2941 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     3001 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     3061 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     3121 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta
     3181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     3301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     3361 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     3421 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3481 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3541 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     3601 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3661 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     3721 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4561 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4621 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4681 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4741 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4801 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4861 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4921 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4981 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5041 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5101 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5161 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5221 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5281 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5341 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5401 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5461 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5521 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5581 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5641 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat
     5701 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     5761 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     5821 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     5881 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     5941 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     6001 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     6061 ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc
     6121 cacccccaat tttgtattta tttatttttt
//
