LOCUS       sequence-228565-       10782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral TET-ON inducible GFP-Lamin A wildtype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Kubben N, Brimacombe KR, Donegan M, Li Z, Misteli T
  TITLE     A high-content imaging-based screening pipeline for the systematic 
            identification of anti-progeroid compounds.
  JOURNAL   Methods. 2015 Sep 1. pii: S1046-2023(15)30070-0. doi: 
            10.1016/j.ymeth.2015.08.024.
  PUBMED    26341717
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Sep 1. pii: S1046-2023(15)30070-0. doi: 
            10.1016/j.ymeth.2015.08.024."
COMMENT     Sequence Label: pLenti CMV TRE3G Neo GFP-Lamin A wildtype
FEATURES             Location/Qualifiers
     source          1..10782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1485..1718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1903..1947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2358..2736
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2366..2384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2438..2456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2474..2492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2510..2528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2546..2564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2582..2600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2645..2669
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2782..2806
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2820..2829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2826..2828,2829..2831,2832..3542)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2826..2828,2829..2831,2832..3542)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2871..2892)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3132..3151)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3479..3500
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5620..5644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5675..6263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5728..5748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6146..6157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6306..6805
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6341..6364)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6738..6757
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6819..7622
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6882..6901)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7492..7511
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7713..7946
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8018..8139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8055..8074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8109..8128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8179..8314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8334..8353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8335..8353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8363..8380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8363..8379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8372..8394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8521..8976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8608..8627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8818..8839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9002..9106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9107..9175,9176..9967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9325..9344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10138..10726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10627..10646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
     2341 gagactagcc tcgagttggc tttactccct atcagtgata gagaacgtat gaagagttta
     2401 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     2461 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     2521 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     2581 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     2641 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     2701 ataccaactt tccgtaccac ttcctaccct cgtaaaccgg ttcgtcgact agtccagtgt
     2761 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa ataccggtcg
     2821 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2881 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2941 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3001 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3061 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3121 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3181 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3241 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3301 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3361 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3421 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3541 agtccggact cagatctcga gatttcgaaa aaggcgccgg gtcgaccgag accccgtccc
     3601 agcggcgcgc cacccgcagc ggggcgcagg ccagctccac tccgctgtcg cccacccgca
     3661 tcacccggct gcaggagaag gaggacctgc aggagctcaa tgatcgcttg gcggtctaca
     3721 tcgaccgtgt gcgctcgctg gaaacggaga acgcagggct gcgccttcgc atcaccgagt
     3781 ctgaagaggt ggtcagccgc gaggtgtccg gcatcaaggc cgcctacgag gccgagctcg
     3841 gggatgcccg caagaccctt gactcagtag ccaaggagcg cgcccgcctg cagctggagc
     3901 tgagcaaagt gcgtgaggag tttaaggagc tgaaagcgcg caataccaag aaggagggtg
     3961 acctgatagc tgctcaggct cggctgaagg acctggaggc tctgctgaac tccaaggagg
     4021 ccgcactgag cactgctctc agtgagaagc gcacgctgga gggcgagctg catgatctgc
     4081 ggggccaggt ggccaagctt gaggcagccc taggtgaggc caagaagcaa cttcaggatg
     4141 agatgctgcg gcgggtggat gctgagaaca ggctgcagac catgaaggag gaactggact
     4201 tccagaagaa catctacagt gaggagctgc gtgagaccaa gcgccgtcat gagacccgac
     4261 tggtggagat tgacaatggg aagcagcgtg agtttgagag ccggctggcg gatgcgctgc
     4321 aggaactgcg ggcccagcat gaggaccagg tggagcagta taagaaggag ctggagaaga
     4381 cttattctgc caagctggac aatgccaggc agtctgctga gaggaacagc aacctggtgg
     4441 gggctgccca cgaggagctg cagcagtcgc gcatccgcat cgacagcctc tctgcccagc
     4501 tcagccagct ccagaagcag ctggcagcca aggaggcgaa gcttcgagac ctggaggact
     4561 cactggcccg tgagcgggac accagccggc ggctgctggc ggaaaaggag cgggagatgg
     4621 ccgagatgcg ggcaaggatg cagcagcagc tggacgagta ccaggagctt ctggacatca
     4681 agctggccct ggacatggag atccacgcct accgcaagct cttggagggc gaggaggaga
     4741 ggctacgcct gtcccccagc cctacctcgc agcgcagccg tggccgtgct tcctctcact
     4801 catcccagac acagggtggg ggcagcgtca ccaaaaagcg caaactggag tccactgaga
     4861 gccgcagcag cttctcacag cacgcacgca ctagcgggcg cgtggccgtg gaggaggtgg
     4921 atgaggaggg caagtttgtc cggctgcgca acaagtccaa tgaggaccag tccatgggca
     4981 attggcagat caagcgccag aatggagatg atcccttgct gacttaccgg ttcccaccaa
     5041 agttcaccct gaaggctggg caggtggtga cgatctgggc tgcaggagct ggggccaccc
     5101 acagcccccc taccgacctg gtgtggaagg cacagaacac ctggggctgc gggaacagcc
     5161 tgcgtacggc tctcatcaac tccactgggg aagaagtggc catgcgcaag ctggtgcgct
     5221 cagtgactgt ggttgaggac gacgaggatg aggatggaga tgacctgctc catcaccacc
     5281 acggctccca ctgcagcagc tcgggggacc ccgctgagta caacctgcgc tcgcgcaccg
     5341 tgctgtgcgg gacctgcggg cagcctgccg acaaggcatc tgccagcggc tcaggagccc
     5401 aggtgggcgg acccatctcc tctggctctt ctgcctccag tgtcacggtc actcgcagct
     5461 accgcagtgt ggggggcagt gggggtggca gcttcgggga caatctggtc acccgctcct
     5521 acctcctggg caactccagc ccccgaaccc agagccccca gaactgcagc atcatgtaat
     5581 ctgtctagaa gaattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag
     5641 tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg
     5701 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5761 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5821 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5881 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5941 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6001 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6061 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6121 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6181 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6241 ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggactag tgatctaatt
     6301 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     6361 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     6421 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     6481 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     6541 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     6601 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     6661 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6721 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6781 ctcttcctca tctccgggcc tttcgacctg cagccaatat gggatcggcc attgaacaag
     6841 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6901 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6961 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7021 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7081 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7141 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7201 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7261 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7321 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     7381 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     7441 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     7501 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     7561 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     7621 gaggggatcc gtcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     7681 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
     7741 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7801 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7861 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7921 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7981 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     8041 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8101 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     8161 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8221 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8281 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     8341 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     8401 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     8461 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     8521 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8581 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8641 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8701 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8761 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8821 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8881 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8941 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     9001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     9061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     9121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     9181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     9241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     9301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     9361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     9421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     9481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     9541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     9601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     9661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     9721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     9781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     9841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     9901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     9961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    10021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    10081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    10141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    10201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    10261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    10321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    10381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    10441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    10501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    10561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    10621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    10681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    10741 ggccttttta cggttcctgg ccttttgctg gccttttgct ca
//
