LOCUS       sequence-228573-        7951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Int5-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2048 to 2082)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-13
FEATURES             Location/Qualifiers
     source          1..7951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2048..2082
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             3805..3816
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5647..5664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5954..5973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6569..6663)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(6761..6829,6830..7621)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7625..7727)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(7904..7947)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtaccta taatgtgtgg atcacacagg aaagtactag attgaacctc
     1921 tactacgagt gagttgagat tacagcctta ctcggtacca aattccagaa aagagacgct
     1981 ttcgagcgtc ttttttcgtt ttggtccctt acgctattat tgaagccagt ctgttaccga
     2041 agtgaaattg acggctagct cagtcctagg tacagtgcta gccagaggaa ggaggctcga
     2101 tgcctggtat gaccaccgaa accggtccgg atccggcagg tctgattgac ctgttttgtc
     2161 gtaaaagcaa agcagttaaa agccgtgcaa atggtgcagg tcagcgtcgt aaacaagaaa
     2221 ttagcattgc agcacaagaa accctgggtc gtaaagttgc agcactgctg ggtatgcagg
     2281 ttcgtcatgt ttggaaagaa gttggtagcg caagccgttt tcgtaaaggt aaagcacgtg
     2341 atgatcagag caaagcactg aaagccctgg aaagcggtga agttggtgca ctgtggtgtt
     2401 atcgtctgga tcgttgggat cgtggtggtg ccggtgcaat tctgaaaatt atcgaaccgg
     2461 aagatggtat gcctcgtcgt ctgctgtttg gttgggatga agataccggt agtccggttc
     2521 tggatagcac caataaacgt gatcgcggtg aactgattcg tcgtgcagaa gaagcacgcg
     2581 aagaagcaga aaaactgagc gaacgtgttc gtgataccaa agcacatcag cgtgaaaatg
     2641 gtgaatgggt taatgcccgt gcaccgtatg gtctgcgtgt tgttctggtt accgttagtg
     2701 atgaagaggg tgatgaatat gatgaacgta aactggcagc agatgatgaa gatgcgggtg
     2761 gtcctgatgg tctgaccaaa gcagaagcag cccgtctggt ttttaccctg ccggttaccg
     2821 atcgtctgag ctatgcaggc accgcacatg caatgaatac ccgtgaaatt ccgagcccga
     2881 ccggtggtcc gtggattgca gttaccgtgc gtgatatgat tcagaatccg gcatatgcgg
     2941 gttggcagac cacaggtcgt caggatggta aacagcgtcg tctgaccttt tataacggtg
     3001 aaggtaaacg tgttagcgtt atgcatggtc ctccgctggt gaccgatgaa gaacaagaag
     3061 ccgcaaaagc agccgttaaa ggtgaagatg gtgttggtgt tccgctggat ggtagcgatc
     3121 atgatacccg tcgcaaacat ctgctgagcg gtcgtatgcg ttgtccgggt tgtggtggta
     3181 gctgtagcta tagcggtaat ggttatcgtt gttggcgtag cagtgtgaaa ggtggttgtc
     3241 cggcaccgac ctatgttgca cgtaaaagcg ttgaagaata tgttgcattt cgttgggcag
     3301 caaaattagc agcaagcgaa ccggatgatc cgtttgttat tgcagttgca gatcgctggg
     3361 cagcactgac ccatccgcag gcaagcgaag atgaaaagta tgcaaaagcc gcagttcgtg
     3421 aagccgaaaa aaatctgggt cgcctgctgc gtgatcgtca gaatggtgtt tatgatggtc
     3481 cggcagaaca gttttttgcc cctgcatatc aagaagcact gagcaccttg caggcagcca
     3541 aagatgcagt tagcgaaagc agcgcaagcg cagcagttga tgttagctgg attgttgata
     3601 gcagcgatta tgaagaactg tggctgcgtg caaccccgac catgcgtaat gcaattattg
     3661 atacctgcat cgatgaaatt tgggttgcaa aaggccagcg tggtcgtccg tttgatggtg
     3721 atgaacgcgt taaaatcaaa tgggcagccc gtacctaaaa cgattacgga ttgctctact
     3781 gtgactgaag tttacaaccc aattattgaa ggccgctaac gcggcctttt tttgtttctg
     3841 gtctccctga ggcacagaga tttactttat tcacgacttc agatacgcac agctaacacc
     3901 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact ttacgggcat
     3961 gcataaggct cgtatgatat attcagggag accacaacgg tttccctcta caaataattt
     4021 tgtttaactt tagtaatttc aacaaaataa ctaggattcg aatgaaagtg gccatttatg
     4081 ttcgtgttag caccgatgaa caggccaaag aaggttttag cattccggca cagcgtgaac
     4141 gtctgcgtgc attttgtgca agccagggtt gggaaattgt gcaagaatat attgaagaag
     4201 gttggagcgc aaaagatctg gatcgtccgc agatgcagcg tctgctgaaa gatatcaaaa
     4261 aaggcaacat tgatattgtg ctggtgtatc gtctggatcg cctgacccgt agcgttctgg
     4321 atctgtatct gctgttacag acctttgaaa aatacaatgt ggcatttcgt agcgccaccg
     4381 aagtttatga taccagcacc gcaatgggtc gtctgtttat taccctggtt gcagcactgg
     4441 cacagtggga acgtgaaaat ctggcagaac gtgttaaatt tggtatcgag cagatgatcg
     4501 atgaaggtaa aaaaccgggt ggtcatagcc cgtatggtta caaatttgat aaagacttca
     4561 attgcaccat tattgaggaa gaagcagacg ttgttcgtat gatctatcgc atgtattgtg
     4621 atggttatgg ctatcgtagc attgcagatc gtctgaatga actgatggtt aaaccgcgta
     4681 ttgccaaaga atggaatcat aatagcgtgc gtgatatcct gaccaacgat atctatattg
     4741 gcacctatcg ttggggtgat aaagttgttc cgaataatca tccgcctatt attagcgaaa
     4801 ccctgttcaa aaaagcccag aaagaaaaag aaaaacgtgg cgttgatcgt aaacgcgttg
     4861 gtaaatttct gtttaccggt ctgttacagt gtggtaattg tggtggccat aaaatgcagg
     4921 gccattttga taaacgtgag cagaaaacct attaccgttg taccaaatgt caccgcatta
     4981 ccaacgaaaa aaacattctg gaaccgctgc tggatgaaat tcagctgctg attaccagca
     5041 aagaatactt tatgagcaaa ttcagcgacc gctatgatca gcaagaggtt gttgatgtta
     5101 gcgcactgac aaaagaactg gaaaaaatca aacgccagaa agagaaatgg tacgatctgt
     5161 atatggatga tcgtaacccg attccgaaag aagaactgtt tgccaaaatt aacgaactga
     5221 acaaaaaaga agaagaaatc tatagcaagc tgagcgaagt ggaagaagat aaagaaccgg
     5281 ttgaagagaa atataaccgc ctgagcaaaa tgatcgattt taaacagcag tttgagcagg
     5341 ccaacgactt taccaaaaaa gagctgctgt tcagcatctt cgaaaagatt gtgatttatc
     5401 gcgagaaagg caagctgaaa aaaatcaccc tggattacac cctgaaataa aatacggtct
     5461 actacagaag ggtggtttat cttactcaac gacgaacaat aaggcctccc taacgggggg
     5521 ccttttttat tgataacaaa aagatttccc tcgtcacgca gtaagtattt atcgtagaag
     5581 atcactttta tcggtttcca gaacaggtaa gagccaatag tgattacttc gcgttatgca
     5641 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5701 tcactcaaag gcggtaatcc tagctagggc gttcggctgc ggcgagcggt atcagctcac
     5761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6061 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6241 aagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6301 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6361 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6421 ctggtagcgg tggttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6541 agggattttg gtcatgactt gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     6601 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcagcagga
     6661 gtccaagcga gctcgatatc actaaataca ttcaaatatc tatccgctca tgagacaata
     6721 accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc aacatttccg
     6781 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6841 gctggtgaaa gtaaaagatg ccgaagatca gttgggtgca cgtgtgggtt acatcgaact
     6901 ggacctcaac agcggtaaga ttcttgagag ttttcgcccc gaagaacgtt tcccaatgat
     6961 gagcactttt aaagttctgc tctgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7021 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7081 agaaaagcat cttacggacg gcatgacagt acgcgaatta tgcagcgctg ccataaccat
     7141 gagtgataac acggcggcca acttacttct gacaacgatc ggaggaccga aggagcttac
     7201 cgcttttttg cacaacatgg gtgatcatgt aactcgcctt gatcgttggg aaccggagct
     7261 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcta tggcaacaac
     7321 gttgcgcaaa ctcttaactg gcgaacttct tactctcgct tcccggcaac aattaataga
     7381 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7441 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7501 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7561 tatggatgaa cgaaatagac agatcgctga gataggtgcc tccctgatta agcattggta
     7621 ataatttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     7681 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     7741 gccagatatc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     7801 tatcacgagg ccctttcgtc ttcacctcga gtgccacctg acgtctaaga aaccattatt
     7861 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
     7921 tccttagctt tcgctaagga tgatttctgg a
//
