LOCUS       sequence-228575-       10631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation 
            mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Kubben N, Brimacombe KR, Donegan M, Li Z, Misteli T
  TITLE     A high-content imaging-based screening pipeline for the systematic 
            identification of anti-progeroid compounds.
  JOURNAL   Methods. 2015 Sep 1. pii: S1046-2023(15)30070-0. doi: 
            10.1016/j.ymeth.2015.08.024.
  PUBMED    26341717
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Sep 1. pii: S1046-2023(15)30070-0. doi: 
            10.1016/j.ymeth.2015.08.024."
COMMENT     Sequence Label: pLenti CMV TRE3G Neo GFP-Progerin C661S
FEATURES             Location/Qualifiers
     source          1..10631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    232..253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(268..273,274..291,292..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     311..333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     330..346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     330..346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     365..385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        367..385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        413..639
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             640..820
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    867..992
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1485..1718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1903..1947
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2214..2331
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2358..2736
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2366..2384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2402..2420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2438..2456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2474..2492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2510..2528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2546..2564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2582..2600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2645..2669
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2782..2806
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2820..2829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2826..2828,2829..2831,2832..3542)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2826..2828,2829..2831,2832..3542)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2871..2892)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3132..3151)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3479..3500
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5469..5493)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5524..6112
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5577..5597)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5995..6006)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6155..6654
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6190..6213)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6587..6606
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6668..7471
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6731..6750)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7341..7360
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7562..7795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7867..7988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7904..7923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7958..7977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8028..8163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8183..8202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8184..8202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8212..8229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8212..8228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8221..8243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8370..8825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8457..8476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8667..8688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8851..8955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8956..9024,9025..9816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9174..9193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9987..10575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10476..10495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      241 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      301 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      361 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
      421 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
      481 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
      541 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
      601 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
      661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
      721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      841 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      901 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      961 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     1021 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     1081 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     1141 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1201 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     1261 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     1321 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     1381 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     1441 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     1501 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     1561 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     1621 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     1681 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     1741 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     1801 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     1861 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     1921 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     1981 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     2041 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     2101 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     2161 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa
     2221 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2281 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
     2341 gagactagcc tcgagttggc tttactccct atcagtgata gagaacgtat gaagagttta
     2401 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     2461 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     2521 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     2581 ctccctatca gtgatagaga acgtataagc tttaggcgtg tacggtgggc gcctataaaa
     2641 gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt
     2701 ataccaactt tccgtaccac ttcctaccct cgtaaaccgg ttcgtcgact agtccagtgt
     2761 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa ataccggtcg
     2821 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2881 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2941 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3001 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3061 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     3121 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3181 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3241 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3301 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3361 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3421 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3481 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3541 agtccggact cagatctcga gatttcgaaa aaggcgccgg gtcgaccgag accccgtccc
     3601 agcggcgcgc cacccgcagc ggggcgcagg ccagctccac tccgctgtcg cccacccgca
     3661 tcacccggct gcaggagaag gaggacctgc aggagctcaa tgatcgcttg gcggtctaca
     3721 tcgaccgtgt gcgctcgctg gaaacggaga acgcagggct gcgccttcgc atcaccgagt
     3781 ctgaagaggt ggtcagccgc gaggtgtccg gcatcaaggc cgcctacgag gccgagctcg
     3841 gggatgcccg caagaccctt gactcagtag ccaaggagcg cgcccgcctg cagctggagc
     3901 tgagcaaagt gcgtgaggag tttaaggagc tgaaagcgcg caataccaag aaggagggtg
     3961 acctgatagc tgctcaggct cggctgaagg acctggaggc tctgctgaac tccaaggagg
     4021 ccgcactgag cactgctctc agtgagaagc gcacgctgga gggcgagctg catgatctgc
     4081 ggggccaggt ggccaagctt gaggcagccc taggtgaggc caagaagcaa cttcaggatg
     4141 agatgctgcg gcgggtggat gctgagaaca ggctgcagac catgaaggag gaactggact
     4201 tccagaagaa catctacagt gaggagctgc gtgagaccaa gcgccgtcat gagacccgac
     4261 tggtggagat tgacaatggg aagcagcgtg agtttgagag ccggctggcg gatgcgctgc
     4321 aggaactgcg ggcccagcat gaggaccagg tggagcagta taagaaggag ctggagaaga
     4381 cttattctgc caagctggac aatgccaggc agtctgctga gaggaacagc aacctggtgg
     4441 gggctgccca cgaggagctg cagcagtcgc gcatccgcat cgacagcctc tctgcccagc
     4501 tcagccagct ccagaagcag ctggcagcca aggaggcgaa gcttcgagac ctggaggact
     4561 cactggcccg tgagcgggac accagccggc ggctgctggc ggaaaaggag cgggagatgg
     4621 ccgagatgcg ggcaaggatg cagcagcagc tggacgagta ccaggagctt ctggacatca
     4681 agctggccct ggacatggag atccacgcct accgcaagct cttggagggc gaggaggaga
     4741 ggctacgcct gtcccccagc cctacctcgc agcgcagccg tggccgtgct tcctctcact
     4801 catcccagac acagggtggg ggcagcgtca ccaaaaagcg caaactggag tccactgaga
     4861 gccgcagcag cttctcacag cacgcacgca ctagcgggcg cgtggccgtg gaggaggtgg
     4921 atgaggaggg caagtttgtc cggctgcgca acaagtccaa tgaggaccag tccatgggca
     4981 attggcagat caagcgccag aatggagatg atcccttgct gacttaccgg ttcccaccaa
     5041 agttcaccct gaaggctggg caggtggtga cgatctgggc tgcaggagct ggggccaccc
     5101 acagcccccc taccgacctg gtgtggaagg cacagaacac ctggggctgc gggaacagcc
     5161 tgcgtacggc tctcatcaac tccactgggg aagaagtggc catgcgcaag ctggtgcgct
     5221 cagtgactgt ggttgaggac gacgaggatg aggatggaga tgacctgctc catcaccacc
     5281 acggctccca ctgcagcagc tcgggggacc ccgctgagta caacctgcgc tcgcgcaccg
     5341 tgctgtgcgg gacctgcggg cagcctgccg acaaggcatc tgccagcggc tcaggagccc
     5401 agagccccca gaacagcagc atcatgtaaa atcactagtg aattcgcggc cgcactcgag
     5461 atatctagac ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
     5521 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5581 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5641 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5701 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5761 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5821 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5881 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5941 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6001 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6061 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     6121 ctgcagatat ccggactagt gatctaattc taccgggtag gggaggcgct tttcccaagg
     6181 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6241 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6301 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6361 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6421 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6481 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6541 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     6601 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     6661 agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6721 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6781 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6841 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6901 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6961 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7021 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7081 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7141 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7201 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7261 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7321 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7381 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7441 cttctatcgc cttcttgacg agttcttctg aggggatccg tcgagggccc ggtaccttta
     7501 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7561 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7801 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7861 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7981 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     8041 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8101 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8161 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8221 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8281 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8341 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8401 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8461 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8521 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8581 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8641 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8701 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8761 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8821 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8881 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8941 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9001 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9061 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9121 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9181 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9241 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9301 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9361 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9421 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9481 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9541 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9601 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9661 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9721 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9781 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10621 ccttttgctc a
//
