LOCUS       sequence-228577-        7912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Tp901-Int5-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 2009 to 2043)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-14
FEATURES             Location/Qualifiers
     source          1..7912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      183..254
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      270..297
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2009..2043
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             3766..3777
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5608..5625)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5915..5934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6530..6624)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(6722..6790,6791..7582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7586..7688)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(7865..7908)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctagataaag ttgtgccgta tccagcggtt accaataata gtctccaggc
      181 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      241 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      301 atatagtaaa gttccaataa gactccaggt atctgtccgt gtaaaaaaga gtattgactt
      361 cgcatctttt tgtaccttaa tgtgtggatc acacaggaaa gtactagatg actaagaaag
      421 tagcaatcta tacacgagta tccactacta accaagcaga ggaaggcttc tcaattgatg
      481 agcaaattga ccgtttaaca aaatatgctg aagcaatggg gtggcaagta tctgatactt
      541 atactgatgc tggtttttca ggggccaaac ttgaacgccc agcaatgcaa agattaatca
      601 acgatatcga gaataaagct tttgatacag ttcttgtata taagctagac cgcctttcac
      661 gtagtgtaag agatactctt tatcttgtta aggatgtgtt cacaaaaaat aaaatagact
      721 ttatctcgct taatgaaagt attgatactt cttctgctat gggtagcttg tttctcacta
      781 ttctttctgc aattaatgag tttgaaagag agaatataaa agaacgcatg actatgggta
      841 aactagggcg agcgaaatct ggtaagtcta tgatgtggac taagacagct tttgggtatt
      901 accacaacag aaagacaggt atattagaaa ttgttccttt acaagctaca atagttgaac
      961 aaatattcac tgattattta tcaggaatat cacttacaaa attaagagat aaactcaatg
     1021 aatctggaca catcggtaaa gatataccgt ggtcttatcg taccctaaga caaacacttg
     1081 ataatccagt ttactgtggt tatatcaaat ttaaggacag cctatttgaa ggtatgcaca
     1141 aaccaattat cccttatgag acttatttaa aagttcaaaa agagctagaa gaaagacaac
     1201 agcagactta tgaaagaaat aacaacccta gacctttcca agctaaatat atgctgtcag
     1261 ggatggcaag gtgcggttac tgtggagcac ctttaaaaat tgttcttggc cacaaaagaa
     1321 aagatggaag ccgcactatg aaatatcact gtgcaaatag atttcctcga aaaacaaaag
     1381 gaattacagt atataatgac aataaaaagt gtgattcagg aacttatgat ttaagtaatt
     1441 tagaaaatac tgttattgac aacctgattg gatttcaaga aaataatgac tccttattga
     1501 aaattatcaa tggcaacaac caacctattc ttgatacttc gtcatttaaa aagcaaattt
     1561 cacagatcga taaaaaaata caaaagaact ctgatttgta cctaaatgat tttatcacta
     1621 tggatgagtt gaaagatcgt actgattccc ttcaggctga gaaaaagctg cttaaagcta
     1681 agattagcga aaataaattt aatgactcta ctgatgtttt tgagttagtt aaaactcagt
     1741 tgggctcaat tccgattaat gaactatcat atgataataa aaagaaaatc gtcaacaacc
     1801 ttgtatcaaa ggttgatgtt actgctgata atgtagatat catatttaaa ttccaactcg
     1861 ctaccggtta aattgaacct ctactacgag tgagttgaga ttacagcctt actcggtacc
     1921 aaattccaga aaagagacgc tttcgagcgt cttttttcgt tttggtccct tacgctatta
     1981 ttgaagccag tctgttaccg aagtgaaatt gacggctagc tcagtcctag gtacagtgct
     2041 agccagagga aggaggctcg atgcctggta tgaccaccga aaccggtccg gatccggcag
     2101 gtctgattga cctgttttgt cgtaaaagca aagcagttaa aagccgtgca aatggtgcag
     2161 gtcagcgtcg taaacaagaa attagcattg cagcacaaga aaccctgggt cgtaaagttg
     2221 cagcactgct gggtatgcag gttcgtcatg tttggaaaga agttggtagc gcaagccgtt
     2281 ttcgtaaagg taaagcacgt gatgatcaga gcaaagcact gaaagccctg gaaagcggtg
     2341 aagttggtgc actgtggtgt tatcgtctgg atcgttggga tcgtggtggt gccggtgcaa
     2401 ttctgaaaat tatcgaaccg gaagatggta tgcctcgtcg tctgctgttt ggttgggatg
     2461 aagataccgg tagtccggtt ctggatagca ccaataaacg tgatcgcggt gaactgattc
     2521 gtcgtgcaga agaagcacgc gaagaagcag aaaaactgag cgaacgtgtt cgtgatacca
     2581 aagcacatca gcgtgaaaat ggtgaatggg ttaatgcccg tgcaccgtat ggtctgcgtg
     2641 ttgttctggt taccgttagt gatgaagagg gtgatgaata tgatgaacgt aaactggcag
     2701 cagatgatga agatgcgggt ggtcctgatg gtctgaccaa agcagaagca gcccgtctgg
     2761 tttttaccct gccggttacc gatcgtctga gctatgcagg caccgcacat gcaatgaata
     2821 cccgtgaaat tccgagcccg accggtggtc cgtggattgc agttaccgtg cgtgatatga
     2881 ttcagaatcc ggcatatgcg ggttggcaga ccacaggtcg tcaggatggt aaacagcgtc
     2941 gtctgacctt ttataacggt gaaggtaaac gtgttagcgt tatgcatggt cctccgctgg
     3001 tgaccgatga agaacaagaa gccgcaaaag cagccgttaa aggtgaagat ggtgttggtg
     3061 ttccgctgga tggtagcgat catgataccc gtcgcaaaca tctgctgagc ggtcgtatgc
     3121 gttgtccggg ttgtggtggt agctgtagct atagcggtaa tggttatcgt tgttggcgta
     3181 gcagtgtgaa aggtggttgt ccggcaccga cctatgttgc acgtaaaagc gttgaagaat
     3241 atgttgcatt tcgttgggca gcaaaattag cagcaagcga accggatgat ccgtttgtta
     3301 ttgcagttgc agatcgctgg gcagcactga cccatccgca ggcaagcgaa gatgaaaagt
     3361 atgcaaaagc cgcagttcgt gaagccgaaa aaaatctggg tcgcctgctg cgtgatcgtc
     3421 agaatggtgt ttatgatggt ccggcagaac agttttttgc ccctgcatat caagaagcac
     3481 tgagcacctt gcaggcagcc aaagatgcag ttagcgaaag cagcgcaagc gcagcagttg
     3541 atgttagctg gattgttgat agcagcgatt atgaagaact gtggctgcgt gcaaccccga
     3601 ccatgcgtaa tgcaattatt gatacctgca tcgatgaaat ttgggttgca aaaggccagc
     3661 gtggtcgtcc gtttgatggt gatgaacgcg ttaaaatcaa atgggcagcc cgtacctaaa
     3721 acgattacgg attgctctac tgtgactgaa gtttacaacc caattattga aggccgctaa
     3781 cgcggccttt ttttgtttct ggtctccctg aggcacagag atttacttta ttcacgactt
     3841 cagatacgca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt
     3901 gctggataac tttacgggca tgcataaggc tcgtatgata tattcaggga gaccacaacg
     3961 gtttccctct acaaataatt ttgtttaact ttagtaattt caacaaaata actaggattc
     4021 gaatgaaagt ggccatttat gttcgtgtta gcaccgatga acaggccaaa gaaggtttta
     4081 gcattccggc acagcgtgaa cgtctgcgtg cattttgtgc aagccagggt tgggaaattg
     4141 tgcaagaata tattgaagaa ggttggagcg caaaagatct ggatcgtccg cagatgcagc
     4201 gtctgctgaa agatatcaaa aaaggcaaca ttgatattgt gctggtgtat cgtctggatc
     4261 gcctgacccg tagcgttctg gatctgtatc tgctgttaca gacctttgaa aaatacaatg
     4321 tggcatttcg tagcgccacc gaagtttatg ataccagcac cgcaatgggt cgtctgttta
     4381 ttaccctggt tgcagcactg gcacagtggg aacgtgaaaa tctggcagaa cgtgttaaat
     4441 ttggtatcga gcagatgatc gatgaaggta aaaaaccggg tggtcatagc ccgtatggtt
     4501 acaaatttga taaagacttc aattgcacca ttattgagga agaagcagac gttgttcgta
     4561 tgatctatcg catgtattgt gatggttatg gctatcgtag cattgcagat cgtctgaatg
     4621 aactgatggt taaaccgcgt attgccaaag aatggaatca taatagcgtg cgtgatatcc
     4681 tgaccaacga tatctatatt ggcacctatc gttggggtga taaagttgtt ccgaataatc
     4741 atccgcctat tattagcgaa accctgttca aaaaagccca gaaagaaaaa gaaaaacgtg
     4801 gcgttgatcg taaacgcgtt ggtaaatttc tgtttaccgg tctgttacag tgtggtaatt
     4861 gtggtggcca taaaatgcag ggccattttg ataaacgtga gcagaaaacc tattaccgtt
     4921 gtaccaaatg tcaccgcatt accaacgaaa aaaacattct ggaaccgctg ctggatgaaa
     4981 ttcagctgct gattaccagc aaagaatact ttatgagcaa attcagcgac cgctatgatc
     5041 agcaagaggt tgttgatgtt agcgcactga caaaagaact ggaaaaaatc aaacgccaga
     5101 aagagaaatg gtacgatctg tatatggatg atcgtaaccc gattccgaaa gaagaactgt
     5161 ttgccaaaat taacgaactg aacaaaaaag aagaagaaat ctatagcaag ctgagcgaag
     5221 tggaagaaga taaagaaccg gttgaagaga aatataaccg cctgagcaaa atgatcgatt
     5281 ttaaacagca gtttgagcag gccaacgact ttaccaaaaa agagctgctg ttcagcatct
     5341 tcgaaaagat tgtgatttat cgcgagaaag gcaagctgaa aaaaatcacc ctggattaca
     5401 ccctgaaata aaatacggtc tactacagaa gggtggttta tcttactcaa cgacgaacaa
     5461 taaggcctcc ctaacggggg gcctttttta ttgataacaa aaagatttcc ctcgtcacgc
     5521 agtaagtatt tatcgtagaa gatcactttt atcggtttcc agaacaggta agagccaata
     5581 gtgattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5641 gctgcggcga gcggtatcag ctcactcaaa ggcggtaatc ctagctaggg cgttcggctg
     5701 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5761 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     5821 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5881 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5941 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6001 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6061 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6121 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6181 gcagcagcca ctggtaacag gaagcagcca ctggtaacag gattagcaga gcgaggtatg
     6241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt gtttgcaagc agcagattac
     6421 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6481 gtggaacgaa aactcacgtt aagggatttt ggtcatgact tgtgcttgga ttctcaccaa
     6541 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     6601 ttactggatc tatcagcagg agtccaagcg agctcgatat cactaaatac attcaaatat
     6661 ctatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa
     6721 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6781 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gccgaagatc agttgggtgc
     6841 acgtgtgggt tacatcgaac tggacctcaa cagcggtaag attcttgaga gttttcgccc
     6901 cgaagaacgt ttcccaatga tgagcacttt taaagttctg ctctgtggcg cggtattatc
     6961 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7021 ggttgagtac tcaccagtca cagaaaagca tcttacggac ggcatgacag tacgcgaatt
     7081 atgcagcgct gccataacca tgagtgataa cacggcggcc aacttacttc tgacaacgat
     7141 cggaggaccg aaggagctta ccgctttttt gcacaacatg ggtgatcatg taactcgcct
     7201 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7261 gcctgtagct atggcaacaa cgttgcgcaa actcttaact ggcgaacttc ttactctcgc
     7321 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7381 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7441 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7501 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7561 ctccctgatt aagcattggt aataatttag cttccttagc tcctgaaaat ctcgataact
     7621 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     7681 gccgatcaac gtctcatttt cgccagatat cgacgtctaa gaaaccatta ttatcatgac
     7741 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacctcg agtgccacct
     7801 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7861 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg ga
//
