LOCUS       sequence-228578-        9414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Tp901-Int5-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3511 to 3545)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-15
FEATURES             Location/Qualifiers
     source          1..9414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3511..3545
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             5268..5279
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7110..7127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7417..7436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8032..8126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(8224..8292,8293..9084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9088..9190)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(9367..9410)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtacctt aatgtgtgga tcacacagga aagtactaga tgactaagaa
     1921 agtagcaatc tatacacgag tatccactac taaccaagca gaggaaggct tctcaattga
     1981 tgagcaaatt gaccgtttaa caaaatatgc tgaagcaatg gggtggcaag tatctgatac
     2041 ttatactgat gctggttttt caggggccaa acttgaacgc ccagcaatgc aaagattaat
     2101 caacgatatc gagaataaag cttttgatac agttcttgta tataagctag accgcctttc
     2161 acgtagtgta agagatactc tttatcttgt taaggatgtg ttcacaaaaa ataaaataga
     2221 ctttatctcg cttaatgaaa gtattgatac ttcttctgct atgggtagct tgtttctcac
     2281 tattctttct gcaattaatg agtttgaaag agagaatata aaagaacgca tgactatggg
     2341 taaactaggg cgagcgaaat ctggtaagtc tatgatgtgg actaagacag cttttgggta
     2401 ttaccacaac agaaagacag gtatattaga aattgttcct ttacaagcta caatagttga
     2461 acaaatattc actgattatt tatcaggaat atcacttaca aaattaagag ataaactcaa
     2521 tgaatctgga cacatcggta aagatatacc gtggtcttat cgtaccctaa gacaaacact
     2581 tgataatcca gtttactgtg gttatatcaa atttaaggac agcctatttg aaggtatgca
     2641 caaaccaatt atcccttatg agacttattt aaaagttcaa aaagagctag aagaaagaca
     2701 acagcagact tatgaaagaa ataacaaccc tagacctttc caagctaaat atatgctgtc
     2761 agggatggca aggtgcggtt actgtggagc acctttaaaa attgttcttg gccacaaaag
     2821 aaaagatgga agccgcacta tgaaatatca ctgtgcaaat agatttcctc gaaaaacaaa
     2881 aggaattaca gtatataatg acaataaaaa gtgtgattca ggaacttatg atttaagtaa
     2941 tttagaaaat actgttattg acaacctgat tggatttcaa gaaaataatg actccttatt
     3001 gaaaattatc aatggcaaca accaacctat tcttgatact tcgtcattta aaaagcaaat
     3061 ttcacagatc gataaaaaaa tacaaaagaa ctctgatttg tacctaaatg attttatcac
     3121 tatggatgag ttgaaagatc gtactgattc ccttcaggct gagaaaaagc tgcttaaagc
     3181 taagattagc gaaaataaat ttaatgactc tactgatgtt tttgagttag ttaaaactca
     3241 gttgggctca attccgatta atgaactatc atatgataat aaaaagaaaa tcgtcaacaa
     3301 ccttgtatca aaggttgatg ttactgctga taatgtagat atcatattta aattccaact
     3361 cgctaccggt taaattgaac ctctactacg agtgagttga gattacagcc ttactcggta
     3421 ccaaattcca gaaaagagac gctttcgagc gtcttttttc gttttggtcc cttacgctat
     3481 tattgaagcc agtctgttac cgaagtgaaa ttgacggcta gctcagtcct aggtacagtg
     3541 ctagccagag gaaggaggct cgatgcctgg tatgaccacc gaaaccggtc cggatccggc
     3601 aggtctgatt gacctgtttt gtcgtaaaag caaagcagtt aaaagccgtg caaatggtgc
     3661 aggtcagcgt cgtaaacaag aaattagcat tgcagcacaa gaaaccctgg gtcgtaaagt
     3721 tgcagcactg ctgggtatgc aggttcgtca tgtttggaaa gaagttggta gcgcaagccg
     3781 ttttcgtaaa ggtaaagcac gtgatgatca gagcaaagca ctgaaagccc tggaaagcgg
     3841 tgaagttggt gcactgtggt gttatcgtct ggatcgttgg gatcgtggtg gtgccggtgc
     3901 aattctgaaa attatcgaac cggaagatgg tatgcctcgt cgtctgctgt ttggttggga
     3961 tgaagatacc ggtagtccgg ttctggatag caccaataaa cgtgatcgcg gtgaactgat
     4021 tcgtcgtgca gaagaagcac gcgaagaagc agaaaaactg agcgaacgtg ttcgtgatac
     4081 caaagcacat cagcgtgaaa atggtgaatg ggttaatgcc cgtgcaccgt atggtctgcg
     4141 tgttgttctg gttaccgtta gtgatgaaga gggtgatgaa tatgatgaac gtaaactggc
     4201 agcagatgat gaagatgcgg gtggtcctga tggtctgacc aaagcagaag cagcccgtct
     4261 ggtttttacc ctgccggtta ccgatcgtct gagctatgca ggcaccgcac atgcaatgaa
     4321 tacccgtgaa attccgagcc cgaccggtgg tccgtggatt gcagttaccg tgcgtgatat
     4381 gattcagaat ccggcatatg cgggttggca gaccacaggt cgtcaggatg gtaaacagcg
     4441 tcgtctgacc ttttataacg gtgaaggtaa acgtgttagc gttatgcatg gtcctccgct
     4501 ggtgaccgat gaagaacaag aagccgcaaa agcagccgtt aaaggtgaag atggtgttgg
     4561 tgttccgctg gatggtagcg atcatgatac ccgtcgcaaa catctgctga gcggtcgtat
     4621 gcgttgtccg ggttgtggtg gtagctgtag ctatagcggt aatggttatc gttgttggcg
     4681 tagcagtgtg aaaggtggtt gtccggcacc gacctatgtt gcacgtaaaa gcgttgaaga
     4741 atatgttgca tttcgttggg cagcaaaatt agcagcaagc gaaccggatg atccgtttgt
     4801 tattgcagtt gcagatcgct gggcagcact gacccatccg caggcaagcg aagatgaaaa
     4861 gtatgcaaaa gccgcagttc gtgaagccga aaaaaatctg ggtcgcctgc tgcgtgatcg
     4921 tcagaatggt gtttatgatg gtccggcaga acagtttttt gcccctgcat atcaagaagc
     4981 actgagcacc ttgcaggcag ccaaagatgc agttagcgaa agcagcgcaa gcgcagcagt
     5041 tgatgttagc tggattgttg atagcagcga ttatgaagaa ctgtggctgc gtgcaacccc
     5101 gaccatgcgt aatgcaatta ttgatacctg catcgatgaa atttgggttg caaaaggcca
     5161 gcgtggtcgt ccgtttgatg gtgatgaacg cgttaaaatc aaatgggcag cccgtaccta
     5221 aaacgattac ggattgctct actgtgactg aagtttacaa cccaattatt gaaggccgct
     5281 aacgcggcct ttttttgttt ctggtctccc tgaggcacag agatttactt tattcacgac
     5341 ttcagatacg cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg
     5401 ttgctggata actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa
     5461 cggtttccct ctacaaataa ttttgtttaa ctttagtaat ttcaacaaaa taactaggat
     5521 tcgaatgaaa gtggccattt atgttcgtgt tagcaccgat gaacaggcca aagaaggttt
     5581 tagcattccg gcacagcgtg aacgtctgcg tgcattttgt gcaagccagg gttgggaaat
     5641 tgtgcaagaa tatattgaag aaggttggag cgcaaaagat ctggatcgtc cgcagatgca
     5701 gcgtctgctg aaagatatca aaaaaggcaa cattgatatt gtgctggtgt atcgtctgga
     5761 tcgcctgacc cgtagcgttc tggatctgta tctgctgtta cagacctttg aaaaatacaa
     5821 tgtggcattt cgtagcgcca ccgaagttta tgataccagc accgcaatgg gtcgtctgtt
     5881 tattaccctg gttgcagcac tggcacagtg ggaacgtgaa aatctggcag aacgtgttaa
     5941 atttggtatc gagcagatga tcgatgaagg taaaaaaccg ggtggtcata gcccgtatgg
     6001 ttacaaattt gataaagact tcaattgcac cattattgag gaagaagcag acgttgttcg
     6061 tatgatctat cgcatgtatt gtgatggtta tggctatcgt agcattgcag atcgtctgaa
     6121 tgaactgatg gttaaaccgc gtattgccaa agaatggaat cataatagcg tgcgtgatat
     6181 cctgaccaac gatatctata ttggcaccta tcgttggggt gataaagttg ttccgaataa
     6241 tcatccgcct attattagcg aaaccctgtt caaaaaagcc cagaaagaaa aagaaaaacg
     6301 tggcgttgat cgtaaacgcg ttggtaaatt tctgtttacc ggtctgttac agtgtggtaa
     6361 ttgtggtggc cataaaatgc agggccattt tgataaacgt gagcagaaaa cctattaccg
     6421 ttgtaccaaa tgtcaccgca ttaccaacga aaaaaacatt ctggaaccgc tgctggatga
     6481 aattcagctg ctgattacca gcaaagaata ctttatgagc aaattcagcg accgctatga
     6541 tcagcaagag gttgttgatg ttagcgcact gacaaaagaa ctggaaaaaa tcaaacgcca
     6601 gaaagagaaa tggtacgatc tgtatatgga tgatcgtaac ccgattccga aagaagaact
     6661 gtttgccaaa attaacgaac tgaacaaaaa agaagaagaa atctatagca agctgagcga
     6721 agtggaagaa gataaagaac cggttgaaga gaaatataac cgcctgagca aaatgatcga
     6781 ttttaaacag cagtttgagc aggccaacga ctttaccaaa aaagagctgc tgttcagcat
     6841 cttcgaaaag attgtgattt atcgcgagaa aggcaagctg aaaaaaatca ccctggatta
     6901 caccctgaaa taaaatacgg tctactacag aagggtggtt tatcttactc aacgacgaac
     6961 aataaggcct ccctaacggg gggccttttt tattgataac aaaaagattt ccctcgtcac
     7021 gcagtaagta tttatcgtag aagatcactt ttatcggttt ccagaacagg taagagccaa
     7081 tagtgattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tcctagctag ggcgttcggc
     7201 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7261 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7681 tggcagcagc cactggtaac aggaagcagc cactggtaac aggattagca gagcgaggta
     7741 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7801 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7861 ttgatccggc aaacaaacca ccgctggtag cggtggtttt ttgtttgcaa gcagcagatt
     7921 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga cttgtgcttg gattctcacc
     8041 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     8101 cattactgga tctatcagca ggagtccaag cgagctcgat atcactaaat acattcaaat
     8161 atctatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8221 aatatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8281 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgccgaaga tcagttgggt
     8341 gcacgtgtgg gttacatcga actggacctc aacagcggta agattcttga gagttttcgc
     8401 cccgaagaac gtttcccaat gatgagcact tttaaagttc tgctctgtgg cgcggtatta
     8461 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8521 ttggttgagt actcaccagt cacagaaaag catcttacgg acggcatgac agtacgcgaa
     8581 ttatgcagcg ctgccataac catgagtgat aacacggcgg ccaacttact tctgacaacg
     8641 atcggaggac cgaaggagct taccgctttt ttgcacaaca tgggtgatca tgtaactcgc
     8701 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8761 atgcctgtag ctatggcaac aacgttgcgc aaactcttaa ctggcgaact tcttactctc
     8821 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8881 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8941 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9001 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9061 gcctccctga ttaagcattg gtaataattt agcttcctta gctcctgaaa atctcgataa
     9121 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     9181 gtgccgatca acgtctcatt ttcgccagat atcgacgtct aagaaaccat tattatcatg
     9241 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagtgccac
     9301 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9361 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tgga
//
