LOCUS       sequence-228586-       11811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destination vector for GreenGate cloning method, contains 2x a set 
            of modules from A-F, ""Driver line"", tissues-specific promoter, 
            Dex-inducible mTurquoise2 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pVL58 - pAPL>>
FEATURES             Location/Qualifiers
     source          1..11811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        complement(1850..2230)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             complement(join(2894..3604,3605..3607,3608..3610))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2936..2957)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3285..3304
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3544..3565
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3695..3741)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    3765..3784
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3817..3836
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3869..3888
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    3921..3940
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             complement(4568..4912)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    9830..9854
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9945..10533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10025..10044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10704..11492)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(11783..11811,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ggatctggct gagaacgcca gctgtgcatg cttggtctag
      601 aatacggtac ccctggattt tggttttagg aattagaaat tttattgata gaagtatttt
      661 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
      721 agaaccctaa ttcccttatc tgggaactac tcacacatta ttatagagag agatagattt
      781 gtagagagag actggtgatt tcagcgggca tgcctgcagg tcgacctgca gccaagctag
      841 gcatgatcta accctctgtc tctggcgtcg cgactgcgaa atttcgcgag ggtttccgag
      901 atggtgattg cgcttcgcag atctccaggc gcgtgggtgc ggacgtagtc agcgccattg
      961 ccgatcgcgt gaagttccgc cgcaaggctc gctggaccca gatcctttac aggaaggcca
     1021 acggtggcgc ccaagaagga tttccgcgac accgagacaa gtagcggaag ccccaacgcc
     1081 gacttcagct tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc ggggctcaag
     1141 aaaaatccca tccccggatc gaggatgagc cggtcggcag cgaccccgct ccgtcgcaag
     1201 gcggaaaccc gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc gggtcgaagg
     1261 tgaccggtgc gggtggcgat gccatcccgc tgcgctgagt gcataaccac cagcctgcag
     1321 tccgcctcag caatatcggg atagagcgca gggtcaggaa atccttggat atcgttcagg
     1381 tagcccacgc cgcgcttgag cgcatagcgc tgggtttccg gttggaagct gtcgattgaa
     1441 acacggtgca tctgatcgga cagggcgtct aagagcggcg caatacgtct gatctcatcg
     1501 gccggcgata caggcctcgc gtccggatgg ctggcggccg gtccgacatc cacgacgtct
     1561 gatccgactc gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc tagccgccgg
     1621 ctctcatcga agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt caccatgcac
     1681 tttactcttc caccattgct tgtaatggaa gtaatgtcag tgttgacctt cttaactggg
     1741 aatccagtca tggatttgag gccgccgaat ggagccaccg cggccgattg caccgtagaa
     1801 gcacggctga ctgtagtcac agctgaagag gatatcatag aagccatggc gatttggtgt
     1861 atcgagattg gttatgaaat tcagatgcta gtgtaatgta ttggtaattt gggaagatat
     1921 aataggaagc aaggctattt atccatttct gaaaaggcga aatggcgtca ccgcgagcgt
     1981 cacgcgcatt ccgttcttgc tgtaaagcgt tgtttggtac acttttgact agcgaggctt
     2041 ggcgtgtcag cgtatctatt caaaagtcgt taatggctgc ggatcaagaa aaagttggaa
     2101 tagaaacaga atacccgcga aattcaggcc cggttgccat gtcctacacg ccgaaataaa
     2161 cgaccaaatt agtagaaaaa taaaaactga ctcggatact tacgtcacgt cttgcgcact
     2221 gatttgaaaa ggatcctagt aacatacact ttcacttgtg atcatcaatt tacttgagag
     2281 tcgtttcttc aaagtttcaa actttccaca acagaaactc tacttaagat gatcaaggca
     2341 ttgtcaaatg aaatgctttg gaaggaagaa actgcctctc cttcgttaaa ccaagctcag
     2401 tatcttgcag tttcctctca tcagacatat caatgcttgg acaaaggtac gaaccatttt
     2461 ctacatttgt gggacattac aggcatttct tagcaacaac gaatgttcat catactcaga
     2521 acctaaaaga ttaacatgtt caagccatcc ttagaaccca acagctcaac actttcgcta
     2581 catctattac tagaggccaa caattggact atttacaagt ttcgcagaac tgcactaaac
     2641 agagtcgagt cactttgcag gcgtattaaa caaaagaagc acagataata aaaacgaagc
     2701 gatgataaag aagaagttcg acttgtcatt agaaagaaag agataacagg aacggaaaca
     2761 tagtagaaca cttattcatc agggattata caaggcccca aaacacaaac caccaaagtt
     2821 ttacatgaaa cgaaacattg aacttcttaa gcataacaga gacgagatgc agctaaagct
     2881 catcatgacc tgacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     2941 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     3001 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     3061 ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg
     3121 ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgcta aagtagttgt
     3181 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     3241 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     3301 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     3361 agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc caggacaggg
     3421 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     3481 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     3541 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     3601 tgctcaccat tgttgagcca ctagtggctg gattgggcag gagatagagg gaaagagcta
     3661 agaagagagc gagtgtgaag gctttcattg ttcgtcctct ccaaatgaaa tgaacttcct
     3721 tatatagagg aagggtcttg cgaagatcga tccactagtc tttcaattgt gagcgctcac
     3781 aattctttct cttccctttc ttctttctag tctttcaatt gtgagcgctc acaattcttt
     3841 ctcttccctt tcttctttct agtctttcaa ttgtgagcgc tcacaattct ttctcttccc
     3901 tttcttcttt ctagtctttc aattgtgagc gctcacaatt ctttctcttc cctttcttct
     3961 ttctagattt cttgagctcg acatatgcat aggttgtgac caggcatcat agcccctaca
     4021 gaacatcttg cctactcata gtggcaaact gtcaaatatg aaaatcgtac aagtatgtac
     4081 atatgaaata aaattgcgga tgtaagaaat taaacttaat ttatttgaaa ttgccattaa
     4141 aaaaagctat caaacataga atatgaaaat agctagttaa ccataaaagg caactacaga
     4201 ttttatgtgg gtgataaaag aataacaaaa catatattcg gcaattctac tgagactaac
     4261 aatgtaaaga tcaatcatca aacagttgac tagaagaaat aagtaacgaa taagtacaaa
     4321 atatatagat cctaaaatat tataagaaca gcaataatat atagtttccg tacagattat
     4381 aaaacacgag caaaactaat ccggtaaaca taacaaagct tatatatttt ttattttttt
     4441 ttgttttgtt ttgttttgtc tcatctctaa accctacgtc aaactcacaa tgacaaaaca
     4501 tatatatctc gttcttacat tcccatgcat cactcataga taaaaattaa taaattgtat
     4561 cacgcagtta ctcttttttt gggtttggtg gggtatcttc atcatcgaat agatagttat
     4621 atacatcatc cattgtagtg gtattaaaca tccctgtagt gattccaaac gcgttatacg
     4681 cagtttggtc cgtccaacca ggtgacagtg gttttgaatt attaccatca tcaattttac
     4741 tagccgtgat ttcattattc atgaagttat catgaacgtt agaggaggca attggttgtg
     4801 aaagcgcttg agaatttgtt tgagttgtta tgaggttcgg accgttgcta ctgttagtga
     4861 aagtgaagga caatgagcta tcagcaatat tcccactttg attaaaattg gcgaattccg
     4921 atccactagt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg
     4981 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
     5041 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc
     5101 ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
     5161 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
     5221 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
     5281 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
     5341 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
     5401 ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt
     5461 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
     5521 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
     5581 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
     5641 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
     5701 ccaagctcga ggtcgcgaca ccgatcagca agctttgttt accagccagc tgctgcgcaa
     5761 cacggttcgg gatgtagttg agctcagcca tggcagcttc aactttttcc cgggttttag
     5821 cgctaacatg tgaagcctgg ttaaccactc tagaaacggt ttgatgagaa acgccggcgt
     5881 attcagcgac gtcgtataac gttaccggtt tgctagtaga tttttgatga aacagaagct
     5941 ttttgatatt tccatttgaa tattttggta tctgattagt gatgatttca gctaacatct
     6001 ctgggaattc aatactcatg gtcttatcca aaaatgtctg gaagcagtag gtaaggagat
     6061 tctcaaccac ctcatgcatg gagtccagaa gctttgtcag ttggtaaaac cgttgccagt
     6121 tctgactgga gttcccttcc cttttgacga tggcttttcc tagctctttg atataagtca
     6181 ttcgaatctc atcaaataac tcttggctct tcagaccttc cttaggaact gaggagagaa
     6241 gcagtaaggt tttcatacag agatactctt cataggatac ctgcaatctt tgtaattcag
     6301 aggagacaaa cagcatgtgt ttacattggt catacatgca gggtagagac attctctgct
     6361 cattaataat cagatcagga gcaaagcaga gcaggtttcc acttgattgt ctgtatgatc
     6421 tccaacccag ggcaaatgcc atgagaaaca tccatgagta ctgtagcagg gtcatttggt
     6481 catccaggtg taagtttctg aagcctggta tcgcctttgc ccatttcact gctgcaatca
     6541 cttgacgccc acctaacatg ttgagtgtgg tcataattct ccatgctgaa tctggaacag
     6601 agctatcata tcctgcatac aacacctcgg gttcaatcac ctccagcagt gacaccaagg
     6661 taggggtgag ctgtggtaat gctgcaggaa ctattgtttt gttaggattt tccgaagtgt
     6721 cttgtgagac tcctgcagtg gcttgctgaa tccctttgat ttttttcttt gtttttcgag
     6781 cttcactagc cattgtgttt aatcgtcttt ggggtcgctt ctgcaaatag aaatgatcaa
     6841 aaactttgaa aaaacacatt tataattggt taaatggatt gaatatatat ttgtgtatgt
     6901 gtgttcatac tgtcggggcc gccgagctga gcgacggcgt ccacaaaacg ctcgtggagt
     6961 tcgacggtcc aacgcaaacg cggtttaggg tcggtggtga ggacaaggcc tgaatcgcct
     7021 tgaacgcaca tagctctgtt ctcatatgaa ccattcatac ttgaaggttt cttagcatgg
     7081 aacattctct ctctctctct ctctgactct ctttctctct ctgaaagagg ggattcaaga
     7141 aacgaatgtg atgcaaaaat gtgagagaat acgatgcgtt tttgaagaga tcagatgacg
     7201 gctttttttt ctctctctcg tcttttttgt tttgtttgta aataaattat ggtttagtta
     7261 aatatgtttt atcgttagta aggaagagaa ctaaagtttc agactagtac acagacgttc
     7321 ctttgtgaaa agatactgtc gctatctttt tcatttgtgt tttttatcct cttagcttga
     7381 atacctttga atttctttca atataaaatg tgtgtaaaag ccactatacg tatacattaa
     7441 gtaaatcatg tctgtctcag attattaaat tactgcgcaa acttaaaata atagtatata
     7501 ttagatatta aattgatatc tttgggggaa attgaatttt gaacatcgga gatctcatat
     7561 caaaacggtt atggatgtga cgacgaaaaa aacaaaaaaa aaagacgaaa aaaagcggtt
     7621 acggatgtat ctaatatata tgcatgattt gttgcatgat ggtttttatt tctttatttc
     7681 ctgatataaa cattaaaatg acttgagata tctaagttaa aatataattc acctgaatca
     7741 aagatattgc acatctatta ttcttatatt tttggtttct ttttctttac taccttataa
     7801 tttagaagta atttttaaat ccaaatttat catacaattg ttctatatac tattttctta
     7861 atcatgcttg atttggagta aatagtaatc aaagatttgt aaatattttc tttataggaa
     7921 aatataaact tttaaagtga atatattaaa gttctatgta ttctattcaa ttaagaccgc
     7981 acgaggagag tcgtatacta gacttatagt ttgtctacat cgtcatatat cgtaattgga
     8041 ggcatacata caaatatctt aacgaaacaa attccacaag gctagggatt ttatcattta
     8101 tggtagggta aacctttttg gctaattatg aggtgaatat acaattctaa gcaatgatga
     8161 gcttcgttat aaattaatag accgtatatt tttggaacaa atttattata tacatgcaat
     8221 ccaaaaccgt taggtacggt ggaatatttg tctacgacaa aaacgttttc cattcatcga
     8281 ttagtaaaag atgtagttta catccgcaat taactagcta gcaaaagaaa aaagaaacaa
     8341 aaaggtgaac aaacgagata attaactaaa ttacttgtat ggacactaca aaccagaaaa
     8401 tcaactttgt taatattgcc ctattagcta ctaaactagc ttatgtatag aacgacgatg
     8461 catttttcaa tatacaaact aaaatcatat ataattcata tacgcaacga tttagattaa
     8521 cccctaaatt caattgcatg catgtaaaat aaatggtcaa cctaaatata tatgagtggg
     8581 aggtcccata gcatatttac atagaacaag aataagtcaa tgaatatggc tgaggctgat
     8641 atgcacacac aaaaaagaga gaatattatc taagaataaa atttgaattg aatcagaaat
     8701 ttattagagg ttatatatta agtattaatt aagggaaagc atgtacatgt taaaatgtag
     8761 accaatacca gtcaatgaat attgaattaa aacaaaaaaa ttaacataag aatatggaac
     8821 aattttttaa agttatacat attataaaca cggactatta atatgtggag aattatgcat
     8881 caatgtgtaa agatagagtt aacgttgggg acctcttctt ctacttatta aagctctttt
     8941 attcttttta tttttaccat atgtttttct tcttttctcc attttaattt attggttaaa
     9001 gcttgctggg gctataacct tttttggctt ttgtaggact gacgattgtg cagctataca
     9061 tggtaacaca tttttcagat attaatttct tggaataaat aaaaaaacag tgtttttctt
     9121 tgccagaaat gttaaaaatc ttggctgtga aacgaattat ttgtacaata aattaaatta
     9181 atcaattacc gcacacaatt ctgaccaaga taaaataaga aaatggttat aacttttgaa
     9241 tttaaatatt aaatcaaagt tcaaattgca tgagaattgg aatcgattct tctcctcttt
     9301 gatgaataaa gatagattct tattgcgcca acaatattaa aaaatgaaaa tacaactccc
     9361 gcgatagatg ccaaccgtat aaacttggcc agccacattt acatagttta ctagactttt
     9421 tattttttct taccactttt tgagtataga tacacacata tattattcct acttatatta
     9481 tcacgatatt tgtgttttca caatcatcat cactattatc tacaaaattg attcatagtg
     9541 atatgatgca tattagcatt agcaaatcat ccgccctaaa atcatttaag ttctcatctt
     9601 tctttattaa tgttttcaat aatgatttta agctaaattg tattttgctt tgaaaaaaag
     9661 aaaccaaagt ttttgttttt agttaaacca agtaaagcta atgtttaact tttagctaat
     9721 tgttgttgaa gcaaactaag agcaggtggt acctggtgcg atcgctgttg gcgcgccgtg
     9781 tttaattaag gttgcggccg cttacttcgt ccgagcctag ttcgagcctt gacaggatat
     9841 attggcgggt aaactaagtc gctgtatgtg tttgtttgag atctcatgtg agcaaaaggc
     9901 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9961 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    10021 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    10081 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    10141 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    10201 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    10261 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    10321 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    10381 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aagaagagtt
    10441 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    10501 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10561 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10621 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10681 tatgtgtaac attggtctag tgattatttg ccgactacct tggtgatctc gcctttcacg
    10741 tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga
    10801 taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
    10861 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
    10921 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
    10981 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
    11041 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
    11101 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
    11161 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
    11221 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
    11281 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
    11341 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
    11401 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
    11461 gtcgatactt cggcgatcac cgcttccctc ataacacccc ttgtattact gtttatgtaa
    11521 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
    11581 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
    11641 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
    11701 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat
    11761 gatggggcga ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc t
//
