LOCUS       sequence-228595-        7755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Tp901-Int7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3511 to 3545)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 7755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-12
FEATURES             Location/Qualifiers
     source          1..7755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3511..3545
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             3609..3620
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5451..5468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5758..5777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6373..6467)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(6565..6633,6634..7425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7429..7531)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(7708..7751)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtacctt aatgtgtgga tcacacagga aagtactaga tgactaagaa
     1921 agtagcaatc tatacacgag tatccactac taaccaagca gaggaaggct tctcaattga
     1981 tgagcaaatt gaccgtttaa caaaatatgc tgaagcaatg gggtggcaag tatctgatac
     2041 ttatactgat gctggttttt caggggccaa acttgaacgc ccagcaatgc aaagattaat
     2101 caacgatatc gagaataaag cttttgatac agttcttgta tataagctag accgcctttc
     2161 acgtagtgta agagatactc tttatcttgt taaggatgtg ttcacaaaaa ataaaataga
     2221 ctttatctcg cttaatgaaa gtattgatac ttcttctgct atgggtagct tgtttctcac
     2281 tattctttct gcaattaatg agtttgaaag agagaatata aaagaacgca tgactatggg
     2341 taaactaggg cgagcgaaat ctggtaagtc tatgatgtgg actaagacag cttttgggta
     2401 ttaccacaac agaaagacag gtatattaga aattgttcct ttacaagcta caatagttga
     2461 acaaatattc actgattatt tatcaggaat atcacttaca aaattaagag ataaactcaa
     2521 tgaatctgga cacatcggta aagatatacc gtggtcttat cgtaccctaa gacaaacact
     2581 tgataatcca gtttactgtg gttatatcaa atttaaggac agcctatttg aaggtatgca
     2641 caaaccaatt atcccttatg agacttattt aaaagttcaa aaagagctag aagaaagaca
     2701 acagcagact tatgaaagaa ataacaaccc tagacctttc caagctaaat atatgctgtc
     2761 agggatggca aggtgcggtt actgtggagc acctttaaaa attgttcttg gccacaaaag
     2821 aaaagatgga agccgcacta tgaaatatca ctgtgcaaat agatttcctc gaaaaacaaa
     2881 aggaattaca gtatataatg acaataaaaa gtgtgattca ggaacttatg atttaagtaa
     2941 tttagaaaat actgttattg acaacctgat tggatttcaa gaaaataatg actccttatt
     3001 gaaaattatc aatggcaaca accaacctat tcttgatact tcgtcattta aaaagcaaat
     3061 ttcacagatc gataaaaaaa tacaaaagaa ctctgatttg tacctaaatg attttatcac
     3121 tatggatgag ttgaaagatc gtactgattc ccttcaggct gagaaaaagc tgcttaaagc
     3181 taagattagc gaaaataaat ttaatgactc tactgatgtt tttgagttag ttaaaactca
     3241 gttgggctca attccgatta atgaactatc atatgataat aaaaagaaaa tcgtcaacaa
     3301 ccttgtatca aaggttgatg ttactgctga taatgtagat atcatattta aattccaact
     3361 cgctaccggt taaattgaac ctctactacg agtgagttga gattacagcc ttactcggta
     3421 ccaaattcca gaaaagagac gctttcgagc gtcttttttc gttttggtcc cttacgctat
     3481 tattgaagcc agtctgttac cgaagtgaaa ttgacggcta gctcagtcct aggtacagtg
     3541 ctagccagag gaaggaggct cgaacgatta cggattgctc tactgtgact gaagtttaca
     3601 acccaattat tgaaggccgc taacgcggcc tttttttgtt tctggtctcc ctgaggcaca
     3661 gagatttact ttattcacga cttcagatac gcacagctaa caccacgtcg tccctatctg
     3721 ctgccctagg tctatgagtg gttgctggat aactttacgg gcatgcataa ggctcgtatg
     3781 atatattcag ggagaccaca acggtttccc tctacaaata attttgttta actttagtaa
     3841 tttcaacaaa ataactagga ttcgaatgaa agtggccatt tatgttcgtg ttagcaccga
     3901 tgaacaggcc aaagaaggtt ttagcattcc ggcacagcgt gaacgtctgc gtgcattttg
     3961 tgcaagccag ggttgggaaa ttgtgcaaga atatattgaa gaaggttgga gcgcaaaaga
     4021 tctggatcgt ccgcagatgc agcgtctgct gaaagatatc aaaaaaggca acattgatat
     4081 tgtgctggtg tatcgtctgg atcgcctgac ccgtagcgtt ctggatctgt atctgctgtt
     4141 acagaccttt gaaaaataca atgtggcatt tcgtagcgcc accgaagttt atgataccag
     4201 caccgcaatg ggtcgtctgt ttattaccct ggttgcagca ctggcacagt gggaacgtga
     4261 aaatctggca gaacgtgtta aatttggtat cgagcagatg atcgatgaag gtaaaaaacc
     4321 gggtggtcat agcccgtatg gttacaaatt tgataaagac ttcaattgca ccattattga
     4381 ggaagaagca gacgttgttc gtatgatcta tcgcatgtat tgtgatggtt atggctatcg
     4441 tagcattgca gatcgtctga atgaactgat ggttaaaccg cgtattgcca aagaatggaa
     4501 tcataatagc gtgcgtgata tcctgaccaa cgatatctat attggcacct atcgttgggg
     4561 tgataaagtt gttccgaata atcatccgcc tattattagc gaaaccctgt tcaaaaaagc
     4621 ccagaaagaa aaagaaaaac gtggcgttga tcgtaaacgc gttggtaaat ttctgtttac
     4681 cggtctgtta cagtgtggta attgtggtgg ccataaaatg cagggccatt ttgataaacg
     4741 tgagcagaaa acctattacc gttgtaccaa atgtcaccgc attaccaacg aaaaaaacat
     4801 tctggaaccg ctgctggatg aaattcagct gctgattacc agcaaagaat actttatgag
     4861 caaattcagc gaccgctatg atcagcaaga ggttgttgat gttagcgcac tgacaaaaga
     4921 actggaaaaa atcaaacgcc agaaagagaa atggtacgat ctgtatatgg atgatcgtaa
     4981 cccgattccg aaagaagaac tgtttgccaa aattaacgaa ctgaacaaaa aagaagaaga
     5041 aatctatagc aagctgagcg aagtggaaga agataaagaa ccggttgaag agaaatataa
     5101 ccgcctgagc aaaatgatcg attttaaaca gcagtttgag caggccaacg actttaccaa
     5161 aaaagagctg ctgttcagca tcttcgaaaa gattgtgatt tatcgcgaga aaggcaagct
     5221 gaaaaaaatc accctggatt acaccctgaa ataaaatacg gtctactaca gaagggtggt
     5281 ttatcttact caacgacgaa caataaggcc tccctaacgg ggggcctttt ttattgataa
     5341 caaaaagatt tccctcgtca cgcagtaagt atttatcgta gaagatcact tttatcggtt
     5401 tccagaacag gtaagagcca atagtgatta cttcgcgtta tgcaggcttc ctcgctcact
     5461 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5521 atcctagcta gggcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggaagcag ccactggtaa
     6061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6241 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6301 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6361 acttgtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     6421 atccagatgg agttctgagg tcattactgg atctatcagc aggagtccaa gcgagctcga
     6481 tatcactaaa tacattcaaa tatctatccg ctcatgagac aataaccctg ataaatgctt
     6541 caataatatt gaaaaaggaa gaatatgagt attcaacatt tccgtgtcgc ccttattccc
     6601 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6661 gatgccgaag atcagttggg tgcacgtgtg ggttacatcg aactggacct caacagcggt
     6721 aagattcttg agagttttcg ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt
     6781 ctgctctgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6841 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6901 gacggcatga cagtacgcga attatgcagc gctgccataa ccatgagtga taacacggcg
     6961 gccaacttac ttctgacaac gatcggagga ccgaaggagc ttaccgcttt tttgcacaac
     7021 atgggtgatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7081 aacgacgagc gtgacaccac gatgcctgta gctatggcaa caacgttgcg caaactctta
     7141 actggcgaac ttcttactct cgcttcccgg caacaattaa tagactggat ggaggcggat
     7201 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7261 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7321 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7381 agacagatcg ctgagatagg tgcctccctg attaagcatt ggtaataatt tagcttcctt
     7441 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     7501 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc
     7561 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     7621 cgtcttcacc tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7681 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     7741 aggatgattt ctgga
//
