LOCUS       sequence-228621-        6436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of human SRSF6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Neueder A, Dumas AA, Benjamin AC, Bates GP
  TITLE     Regulatory mechanisms of incomplete huntingtin mRNA splicing.
  JOURNAL   Nat Commun. 2018 Sep 27;9(1):3955. doi: 10.1038/s41467-018-06281-3.
  PUBMED    30262848
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-09-27"; volume: "9(1):3955. doi"; pages: " 
            10.1038/s41467-018-06281-3"
COMMENT     Sequence Label: pcDNA3 - hSRSF6
FEATURES             Location/Qualifiers
     source          1..6436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1988..2006)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1989..2006)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2026..2043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2032..2256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2302..2730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2389..2408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2599..2620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2739..2759)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2744..3074
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2925..3060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2987..3006
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3141..3935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3195..3214)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3805..3824
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4109..4230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4146..4165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4200..4219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4279..4295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4279..4295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4292..4314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4303..4319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4327..4333,4334..4351,4352..4357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4372..4393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4510..4527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4681..5269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4761..4780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5440..6231,6232..6300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6063..6082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6301..6405)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccgccaccat gccgcgcgtc tacataggac gcctgagcta caacgtccgg
      961 gagaaggaca tccagcgctt tttcagtggc tatggccgcc tcctcgaagt agacctcaaa
     1021 aatgggtacg gcttcgtgga gttcgaggac tcccgcgacg ccgacgacgc cgtttacgag
     1081 ctgaacggca aggagctctg cggcgagcgc gtgatcgtag agcacgcccg gggcccgcgt
     1141 cgcgatcgcg acggctacag ctacggaagc cgcagtggtg gaggtggata cagcagtcgg
     1201 agaacatctg gcagagacaa atacggacca cctgttcgta cagaatacag gcttattgta
     1261 gaaaatcttt ctagtcggtg cagttggcaa gatttaaagg attttatgcg acaagcaggt
     1321 gaagtaacct atgcggatgc ccacaaggaa cgaacaaatg agggtgtaat tgagtttcgc
     1381 tcctactctg acatgaagcg tgctttggac aaactggatg gcacagaaat aaatggcaga
     1441 aatattaggc ttattgaaga taagccacgc acaagccata ggcgatctta ctctggaagc
     1501 agatccaggt ctcgatctag aagacggtca cgaagtagga gtcgcaggag cagccgcagt
     1561 agatctcgaa gtatctcaaa aagtcgctcc cgttccaggt cgcggagcaa aggtcgatca
     1621 cgttctcgat caaaaggcag gaaatctaga tcaaagagca aatctaagcc caagtctgat
     1681 cggggctccc attcacattc tcgaagcaga tctaaggatg agtatgagaa atctcgaagc
     1741 aggtctcggt cccgatcccc taaagaaaat ggaaagggtg atataaagtc aaaatccaga
     1801 tcaaggagcc agtcccgttc caattcgccg ctacctgttc caccctcaaa ggcccgttct
     1861 gtgtcccctc caccaaaaag agctacttca agatcccgtt ctagatctcg ctcaaagtca
     1921 agatcaaggt ccaggtcgag ttccagagat taagcggccg ctcgagcatg catctagagg
     1981 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     2041 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2101 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2161 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     2221 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     2281 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2341 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2401 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     2461 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2521 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2581 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2641 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     2701 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     2761 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     2821 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2881 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2941 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3001 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3061 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     3121 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3181 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3241 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3301 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3361 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3421 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3481 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3541 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3601 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3661 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3721 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3781 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3841 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3901 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     3961 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4021 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4081 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4201 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4261 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4861 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5101 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5341 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5401 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5461 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5521 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5581 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5641 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5701 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5761 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5821 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5881 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5941 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6001 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6061 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6121 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6181 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6241 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6301 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6361 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6421 agtgccacct gacgtc
//
