LOCUS       sequence-228635-       11150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector experessing SLC7A11 insensitive to hairpin 
            TRCN0000288926 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Briggs KJ, Koivunen P, Cao S, Backus KM, Olenchock BA, Patel H, 
            Zhang Q, Signoretti S, Gerfen GJ, Richardson AL, Witkiewicz AK, 
            Cravatt BF, Clardy J, Kaelin WG Jr
  TITLE     Paracrine Induction of HIF by Glutamate in Breast Cancer: EglN1 
            Senses Cysteine.
  JOURNAL   Cell. 2016 Jun 30;166(1):126-39. doi: 10.1016/j.cell.2016.05.042.
  PUBMED    27368101
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-30- 
            30"; volume: "166"; issue: "1"; pages: "126-39"
COMMENT     Sequence Label: pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro
FEATURES             Location/Qualifiers
     source          1..11150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    173..290
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        383..762
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        763..966
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        1011..1029
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1106..1130
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2637..2661)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2681..2704
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2818..3370
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2962..2979)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3189..3208
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3373..3402
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3507..3524
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3540..4565
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3540..4565
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4596..4629)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4685..5273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4738..4758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5156..5167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5276..5292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5277..5293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5482..5715
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5787..5908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5824..5843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5878..5897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5948..6083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6104..6122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6145..6503)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6185..6206)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6397..6416
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6530..6634
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6636..6993
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6636..6827
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6659..6679)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6844..6979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7028..7822
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7028..7822
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7082..7101)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7692..7711
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8010..8029)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8054..8101
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8430..9018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8919..8938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9193..9496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9498..9696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9714..9894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9941..10066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10563..10796
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10981..11025
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
       61 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
      121 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
      181 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      241 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      301 acagggacag cagagatcca gtttggttag taccgggccc gctctagccc tagatttaac
      361 ccaacagaag gctcgacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      421 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      481 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      541 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      601 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      661 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      721 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      781 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      841 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      901 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      961 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     1021 cactataggg agacccaagc ttggtaccga gctcggatcc tagactgccg gatcgaattg
     1081 tcgacgaatt cggatctggc caatcacaag tttgtacaaa aaagcaggct tcatggtcag
     1141 aaagcctgtt gtgtccacca tctccaaagg aggttacctg cagggaaatg ttaacgggag
     1201 gctgccttcc ctgggcaaca aggagccacc tgggcaggag aaagtgcagc tgaagaggaa
     1261 agtcacttta ctgaggggag tctccattat cattggcacc atcattggag caggaatctt
     1321 catctctcct aagggcgtgc tccagaacac gggcagcgtg ggcatgtctc tgaccatctg
     1381 gacggtgtgt ggggtcctgt cactatttgg agctttgtct tatgctgaat tgggaacaac
     1441 tataaagaaa tctggaggtc attacacata tattttggaa gtctttggtc cattaccagc
     1501 ttttgtacga gtctgggtgg aactcctcat aatacgccct gcagctactg ctgtgatatc
     1561 cctggcattt ggacgctaca ttctggaacc attttttatt caatgtgaaa tccctgaact
     1621 tgcgatcaag ctcattacag ctgtgggcat aactgtagtg atggtcctaa atagcatgag
     1681 tgtcagctgg agcgcccgga tccagatttt cttaaccttt tgcaagctca cagcaattct
     1741 gataattata gtgcccggcg tgatgcaatt gattaaaggt caaacgcaga actttaaaga
     1801 cgccttttca ggaagagatt caagtattac gcggttgcca ctggcttttt attatggaat
     1861 gtatgcatat gctggctggt tttacctcaa ctttgttact gaagaagtag aaaaccctga
     1921 aaaaaccatt ccccttgcaa tatgtatatc catggccatt gtcaccattg gctatgtgct
     1981 gacaaatgtg gcctacttta cgaccattaa tgctgaggag ctgctgcttt caaatgcagt
     2041 ggcagtgacc ttttctgagc ggctactggg aaatttctca ttagcagttc cgatctttgt
     2101 tgccctctcc tgctttggct ccatgaacgg tggtgtgttt gctgtctcca ggttattcta
     2161 tgttgcgtct cgagagggtc accttccaga aatcctctcc atgattcatg tccgcaagca
     2221 cactcctcta ccagctgtta ttgttttgca ccctttgaca atgataatgc tcttctctgg
     2281 agacctcgac agtcttttga atttcctcag ttttgccagg tggcttttta ttgggctggc
     2341 agttgctggg ctgatttatc ttcgatacaa atgcccagat atgcatcgtc ctttcaaggt
     2401 gccactgttc atcccagctt tgttttcctt cacatgcctc ttcatggttg ccctttccct
     2461 ctattcggac ccatttagta cagggattgg cttcgtcatc actctgactg gagtccctgc
     2521 gtattatctc tttattatat gggacaagaa acccaggtgg tttagaataa tgtcagagaa
     2581 aataaccaga acattacaaa taatactgga agttgtacca gaagaagata agttaaaccc
     2641 agctttcttg tacaaagtgg tgggaggagg tggatctggc gactacaagg acgacgatga
     2701 caagtgactc gaggtttaaa ctatagagta cgagccatga taactgcagg tcgagcatgc
     2761 atctagggcg gccaattccg cccctctccc cccccccctc tccctccccc ccccctaacg
     2821 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     2881 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     2941 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     3001 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     3061 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     3121 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     3181 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     3241 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     3301 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     3361 atcgataata tggaacaaaa acttatttct gaagaagact tggacaccaa actttcctgc
     3421 cgctcgattt ctccacccag gtgaactcct ccctcacctc cccgacgggg cgaggctcca
     3481 tggctgcccc ctcgctgcac ccgtccctgg tgccacgcgg ttccatggga tcgtttcgca
     3541 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     3601 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     3661 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     3721 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     3781 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     3841 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     3901 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     3961 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     4021 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     4081 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     4141 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     4201 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     4261 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     4321 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     4381 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     4441 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     4501 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     4561 aatagtgtac aagtaggaat tcgtcgaggg acctaataac ttcgtatagc atacattata
     4621 cgaagttata catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat
     4681 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4741 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4801 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4861 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4921 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4981 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5041 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5101 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5161 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5221 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     5281 ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     5341 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     5401 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5461 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     5521 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5581 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5641 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5701 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5761 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5821 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5881 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5941 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6001 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6061 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6121 tagcatgtga gcaaaaggcc agcataaatc aaaagaatag accgagatag ggttgagtgt
     6181 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6241 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6301 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6361 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6421 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6481 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6541 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6601 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     6661 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6721 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6781 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6841 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6901 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6961 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     7021 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     7081 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     7141 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     7201 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7261 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7321 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7381 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7441 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     7501 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     7561 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     7621 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7681 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7741 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7801 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     7861 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7921 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7981 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     8041 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     8101 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     8161 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     8221 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     8281 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8341 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9061 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9121 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     9181 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     9241 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     9301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     9361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     9421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     9481 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     9541 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     9601 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     9661 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     9721 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9781 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9841 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     9901 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     9961 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    10021 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    10081 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    10141 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    10201 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10261 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10321 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10381 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
    10441 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10501 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10561 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10621 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10681 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    10741 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10801 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10861 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    10921 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10981 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11041 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11101 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
//
