LOCUS       sequence-228636-        6143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   de Blas, Angel
  TITLE     GABAA receptor subunit plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Myc-gamma1 (in pCAGGS)
FEATURES             Location/Qualifiers
     source          1..6143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..279
                     /label=chicken beta-actin promoter
     intron          280..1296
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1220..1242
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1304..1323
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1468..1497
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2782..2801)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2847..2902
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2901..2920)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3263..3279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3263..3279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3276..3298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3287..3303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3311..3317,3318..3335,3336..3341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3356..3377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3436..3631
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3482..3617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3637..3771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3674..3693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3728..3747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3839..3856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4010..4598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4090..4109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4769..5560,5561..5629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5392..5411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5630..5734)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(5765..6143,1)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
       61 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
      121 gngcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
      181 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      241 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      301 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      361 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      421 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      481 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      541 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      601 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      661 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
      721 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
      781 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
      841 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      901 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
      961 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     1021 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     1081 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1141 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1201 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1261 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1321 gtgctgtctc atcattttgg caaagaattc atgggttctg ggaaagtctt ccttttctct
     1381 ccttccctcc tgtggagtca aactagagga gtgaggttga tattcttgtt actaaccctg
     1441 catctgggaa actgcattga taaagcagaa cagaagttga tttccgaaga agacctcgat
     1501 gatgaagatg atgaagattt aactatgaac aaaacatggg tcttggcacc taaaattcat
     1561 gaaggagata tcacacagat tctcaactct ttacttcaag gctatgataa caaacttcgt
     1621 ccagatatag gcgtgagacc cacagtaatt gaaactgatg tttatgtaaa cagcattgga
     1681 cctgttgatc ccataaacat ggagtacacg atagacatca tttttgccca gacctggttt
     1741 gatagccgtt taaagttcaa cagcaccatg aaagtcctta tgcttaacag caacatggtg
     1801 ggaaaaattt ggatccctga cacgttcttc aggaactcaa ggaaatctga tgcgcactgg
     1861 ataacaacgc ccaatcgcct gctgcggata tggagtgatg gaagggttct ctacactctg
     1921 agattaacaa ttaatgcaga atgctacctt caactccata actttcctat ggatgaacat
     1981 tcctgtccac tggaattttc aagctatgga taccctaaaa atgaaattga gtacaagtgg
     2041 aaaaagccct cagtggaagt agctgatcct aagtactgga gattgtatca gtttgccttt
     2101 gtagggttaa gaaattcaac cgaaatctct cacacaatct ccggagatta tattatcatg
     2161 actatctttt ttgacctgag cagacggatg ggctatttca caattcagac ctacattccg
     2221 tgcattctaa cagttgttct ttcctgggtg tctttttgga tcaacaaaga tgctgtacct
     2281 gctagaacat ccctgggtat cactacggtt ttgactatga caaccctcag tacaatcgct
     2341 agaaaatctt tacctaaggt ttcttacgtg acagcaatgg atctctttgt ctctgtgtgt
     2401 ttcatttttg tgtttgcagc actcatggag tatggaacct tgcattattt tactagtaac
     2461 aataaaggaa aaaccaccag agacaggaag ctgaaaagca aaacttcggt atccccaggt
     2521 ctccatgctg gatctactct gattcccatg aacaacattt ctatgcctca aggggaagat
     2581 gattatggtt accagtgctt ggagggcaaa gactgtgcta cctttttctg ttgctttgaa
     2641 gactgcagaa ctgggtcctg gagagaaggg cgaatacaca tacgcattgc caaaatcgac
     2701 tcctactcca ggatcttttt cccaacagct tttgccttgt tcaatctggt ttactgggtt
     2761 ggatatctat acttataaag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     2821 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     2881 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     2941 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     3001 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     3061 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     3121 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     3181 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     3241 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     3301 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     3361 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     3421 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     3481 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3541 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3601 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     3661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3721 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     3781 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4201 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4441 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5761 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     5821 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5881 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5941 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     6001 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     6061 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     6121 gtattagtca tcgctattac cat
//
