LOCUS       sequence-228649-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ASCL1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ASCL1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagtctt ctgctaaaat ggagtccgga ggcgcgggac aacaaccaca accgcaacca
       61 caacaaccct tcctgccgcc ggccgcatgt tttttcgcga ccgctgctgc tgctgcagcg
      121 gcggcggctg ctgccgccgc gcaatccgcc caacagcaac aacaacaaca gcagcagcag
      181 caacaagcgc ctcaacttcg acccgctgca gacgggcagc cctcaggggg agggcacaag
      241 agcgctccga agcaggttaa aaggcagagg agcagtagtc ccgaactgat gcgatgtaag
      301 aggcgcctca attttagcgg ttttggttac tctttgcccc agcagcagcc ggctgccgta
      361 gctcgccgaa atgagcggga aaggaaccgc gttaaacttg tgaatctcgg tttcgcgaca
      421 cttcgagagc acgtaccaaa tggggcagct aacaagaaaa tgagtaaagt tgagacactg
      481 cggtctgcag tggagtatat tagagctctt caacaattgc ttgacgagca cgatgccgta
      541 tcagccgcat ttcaagccgg ggtgctgtcc ccaacaatat ctccgaacta cagcaatgat
      601 cttaatagca tggcgggaag tcccgtttcc tcctactcct ctgatgaggg cagctacgac
      661 cctctcagtc ccgaggagca agagcttctt gacttcacta actggttc
//
