LOCUS       sequence-228652-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ASCL3 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ASCL3_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(545..569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgatggaca acagaggcaa ctctagtcta cctgacaaac ttcctatctt ccctgattct
       61 gcccgcttgc cacttaccag gtccttctat ctggagccca tggtcacttt ccacgtgcac
      121 ccagaggccc cggtgtcatc tccttactct gaggagctgc cacggctgcc ttttcccagc
      181 gactctctta tcctgggaaa ttacagtgaa ccctgcccct tctctttccc gatgccttat
      241 ccaaattaca gagggtgcga gtactcctac gggccagcct tcacccggaa aaggaatgag
      301 cgggaaaggc agcgggtgaa atgtgtcaat gaaggctacg cccagctccg acatcatctg
      361 ccagaggagt atttggagaa gcgactcagc aaagtggaaa ccctcagagc tgcgatcaag
      421 tacattaact acctgcagtc tcttctgtac cctgataaag ctgagacaaa gaataaccct
      481 ggaaaagttt cctccatgat agcaaccacc agccaccatg ctgaccctat gttcagaatt
      541 gtttgcccaa ctttcttgta caaagttgtc ccc
//
