LOCUS       sequence-228655-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ASCL4 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ASCL4_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagacgc gtaaaccggc ggaacggctg gccttgccat actcgctgcg caccgcgccc
       61 ctgggcgttc cggggaccct gcccggactc ccgcggaggg accccctcag ggtcgccctg
      121 cgtctggacg ccgcgtgctg ggagtgggcg cgcagcggct gcgcacgggg atggcagtac
      181 ttgcccgtgc cgctggacag cgccttcgag cccgccttcc tccgcaagcg caacgagcgc
      241 gagcggcagc gggtgcgctg cgtgaacgag ggctatgcgc gcctccgaga ccacctgccc
      301 cgggagctgg cagacaagcg cctcagcaaa gtggagacgc tccgcgctgc catcgactac
      361 atcaagcacc tgcaggagct gctggagcgc caggcctggg ggctcgaggg cgcggccggc
      421 gccgtccccc agcgcagggc ggaatgcaac agcgacgggg agtccaaggc ctcttcggcg
      481 ccttcgccca gcagcgagcc cgaggagggg ggcagc
//
