LOCUS       sequence-228658-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ASCL5 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ASCL5_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccgatgg gggcagcaga aagaggtgct gggccccaat catctgcagc accatgggct
       61 ggttcagaaa aggcggcaaa gagagggcca tcaaaaagct ggtacccaag agctgctgca
      121 tctgatgtca cgtgcccgac tggtggtgat ggagctgacc caaaacctgg accttttgga
      181 ggtggtttag ctttagggcc tgcgcccaga ggaacaatga ataataattt ctgcagggcc
      241 cttgttgaca gaaggccttt aggaccccct tcatgtatgc aattaggtgt aatgccaccg
      301 ccaagacaag cgcccctccc gccggctgaa ccccttggaa atgtaccttt cctcctatac
      361 cctggcccag ctgaaccacc atattatgat gcatatgctg gtgttttccc atatgtgcct
      421 ttccctggtg cttttggtgt atatgaatac ccttttgagc cggcttttat ccaaaagagg
      481 aatgaaagag agagacagag agtgaagtgt gtgaatgaag gatacgccag attgagaggc
      541 catttgcctg gtgccctggc agaaaagaga ttatcaaaag ttgaaaccct gagggcggca
      601 atcagatata taaaatacct ccaagaactc ctttcatcag cacctgatgg atcgacacca
      661 ccggcttcaa gaggtttacc tggaactgga ccatgccctg caccgcctgc tacaccaagg
      721 ccagacagac ctggagatgg agaagcaaga gcaccttctt cccttgtccc tgaatcttct
      781 gaatcatcat gtttttcgcc ttcccctttt ttagaaagtg aagaatcctg gca
//
