LOCUS       sequence-228673-        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ESRRG in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ESRRG_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             126..137
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgtcaaaca aagatcgaca cattgattcc agctgttcgt ccttcatcaa gacggaacct
       61 tccagcccag cctccctgac ggacagcgtc aaccaccaca gccctggtgg ctcttcagac
      121 gccagtggga gctacagttc aaccatgaat ggccatcaga acggacttga ctcgccacct
      181 ctctaccctt ctgctcctat cctgggaggt agtgggcctg tcaggaaact gtatgatgac
      241 tgctccagca ccattgttga agatccccag accaagtgtg aatacatgct caactcgatg
      301 cccaagagac tgtgtttagt gtgtggtgac atcgcttctg ggtaccacta tggggtagca
      361 tcatgtgaag cctgcaaggc attcttcaag aggacaattc aaggcaatat agaatacagc
      421 tgccctgcca cgaatgaatg tgaaatcaca aagcgcagac gtaaatcctg ccaggcttgc
      481 cgcttcatga agtgtttaaa agtgggcatg ctgaaagaag gggtgcgtct tgacagagta
      541 cgtggaggtc ggcagaagta caagcgcagg atagatgcgg agaacagccc atacctgaac
      601 cctcagctgg ttcagccagc caaaaagcca ttgctctggt ctgatcctgc agataacaag
      661 attgtctcac atttgttggt ggctgaaccg gagaagatct atgccatgcc tgaccctact
      721 gtccccgaca gtgacatcaa agccctcact acactgtgtg acttggccga ccgagagttg
      781 gtggttatca ttggatgggc gaagcatatt ccaggcttct ccacgctgtc cctggcggac
      841 cagatgagcc ttctgcagag tgcttggatg gaaattttga tccttggtgt cgtataccgg
      901 tctctttcgt ttgaggatga acttgtctat gcagacgatt atataatgga cgaagaccag
      961 tccaaattag caggccttct tgatctaaat aatgctatcc tgcagctggt aaagaaatac
     1021 aagagcatga agctggaaaa agaagaattt gtcaccctca aagctatagc tcttgctaat
     1081 tcagactcca tgcacataga agatgttgaa gccgttcaga agcttcagga tgtcttacat
     1141 gaagcgctgc aggattatga agctggccag cacatggaag accctcgtcg agctggcaag
     1201 atgctgatga cactgccact cctgaggcag acctctacca aggccgtgca gcatttctac
     1261 aacatcaaac tagaaggcaa agtcccaatg cacaaacttt ttttggaaat gttggaggcc
     1321 aaggtc
//
