LOCUS       sequence-228676-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ETV2 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ETV2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             794..805
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggatcttt ggaactggga tgaagcttcc cctcaagaag ttccccccgg aaataaactc
       61 gcggggcttg gaagactccc tcgccttccg caacgcgtct ggggcggatg ccctggtgga
      121 gcctcagcgg acccaaaccc tttgtctcca gcggaggggg caaagttggg tttctgcttc
      181 ccggatcttg ctttgcaagg cgatactcca acggcgacgg cagagacctg ttggaaaggc
      241 accagtagct ccctggccag ctttccgcag ctcgattggg ggtcagccct tctccatccc
      301 gaagttccct ggggggcgga acccgactcc caagcccttc cctggagtgg tgattggaca
      361 gatatggcat gcacagcctg ggacagttgg tccggggcgt cacagacatt gggaccagcc
      421 ccacttggac cggggcctat ccccgcagca ggaagcgaag gagctgctgg tcagaactgt
      481 gtgcccgtgg ctggtgaggc taccagttgg tccagggccc aggcagcagg cagtaacacc
      541 agctgggatt gctcagtggg gcctgacggg gatacttatt ggggctctgg tcttggtgga
      601 gaaccgagaa cggactgtac gataagttgg ggcggtccag ctgggcctga ttgtactacg
      661 tcatggaatc ctggcttgca cgccggcggc acgacaagcc ttaagagata tcaaagttca
      721 gcccttacag tttgctcaga accttccccg caaagtgacc gagcgtcact ggcgcgatgt
      781 cctaaaacta atcatcgagg gccgatccag ttgtggcagt ttttgcttga actccttcac
      841 gatggcgcga ggagcagttg catcagatgg accggtaaca gcagggagtt ccaattgtgt
      901 gaccccaagg aagtggctcg actgtggggt gagcgcaaac ggaagcctgg tatgaattac
      961 gaaaagttga gtaggggttt gcgatattac tataggcgcg acatcgttcg aaagtccggt
     1021 ggtcgaaagt acacatacag attcggcggt cgcgtaccat ctcttgcata ccctgattgc
     1081 gcaggcgggg gtaggggtgc ggaaacacaa
//
