LOCUS       sequence-228679-        1356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express FLI1 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_FLI1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggacggga ctattaagga ggctctgtcg gtggtgagcg acgaccagtc cctctttgac
       61 tcagcgtacg gagcggcagc ccatctcccc aaggccgaca tgactgcctc ggggagtcct
      121 gactacgggc agccccacaa gatcaacccc ctcccaccac agcaggagtg gatcaatcag
      181 ccagtgaggg tcaacgtcaa gcgggagtat gaccacatga atggatccag ggagtctccg
      241 gtggactgca gcgttagcaa atgcagcaag ctggtgggcg gaggcgagtc caaccccatg
      301 aactacaaca gctatatgga cgagaagaat ggcccccctc ctcccaacat gaccaccaac
      361 gagaggagag tcatcgtccc cgcagacccc acactgtgga cacaggagca tgtgaggcaa
      421 tggctggagt gggccataaa ggagtacagc ttgatggaga tcgacacatc ctttttccag
      481 aacatggatg gcaaggaact gtgtaaaatg aacaaggagg acttcctccg cgccaccacc
      541 ctctacaaca cggaagtgct gttgtcacac ctcagttacc tcagggaaag ttcactgctg
      601 gcctataata caacctccca caccgaccaa tcctcacgat tgagtgtcaa agaagaccct
      661 tcttatgact cagtcagaag aggagcttgg ggcaataaca tgaattctgg cctcaacaaa
      721 agtcctcccc ttggaggggc acaaacgatc agtaagaata cagagcaacg gccccagcca
      781 gatccgtatc agatcctggg cccgaccagc agtcgcctag ccaaccctgg aagcgggcag
      841 atccagctgt ggcaattcct cctggagctg ctctccgaca gcgccaacgc cagctgtatc
      901 acctgggagg ggaccaacgg ggagttcaaa atgacggacc ccgatgaggt ggccaggcgc
      961 tggggcgagc ggaaaagcaa gcccaacatg aattacgaca agctgagccg ggccctccgt
     1021 tattactatg ataaaaacat tatgaccaaa gtgcacggca aaagatatgc ttacaaattt
     1081 gacttccacg gcattgccca ggctctgcag ccacatccga ccgagtcgtc catgtacaag
     1141 tacccttctg acatctccta catgccttcc taccatgccc accagcagaa ggtgaacttt
     1201 gtccctcccc atccatcctc catgcctgtc acttcctcca gcttctttgg agccgcatca
     1261 caatactgga cctcccccac ggggggaatc taccccaacc ccaacgtccc ccgccatcct
     1321 aacacccacg tgccttcaca cttaggcagc tactac
//
