LOCUS       sequence-228682-        1416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express FOXA1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_FOXA1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttgggca ccgtgaagat ggaggggcat gagacaagcg actggaattc ctactacgcg
       61 gatacccaag aagcgtattc ttcagttccc gtaagcaata tgaactccgg attggggagc
      121 atgaatagta tgaacacgta tatgacaatg aatacgatga ccaccagcgg caacatgaca
      181 ccggcctcct ttaatatgtc atatgcgaac cctggtcttg gcgctggcct ctcaccaggt
      241 gcggtcgctg gaatgcccgg ggggagcgcc ggagcgatga actccatgac cgctgcgggc
      301 gtgacggcca tgggtacggc cctgtcaccc agtggaatgg gagctatggg ggcccagcaa
      361 gccgcctcaa tgaatggatt ggggccctat gccgcggcga tgaatccctg catgtcccct
      421 atggcttatg cccccagcaa tttgggtcgc agtagagccg gcggtggtgg cgatgccaaa
      481 accttcaagc gaagttatcc tcatgcgaag cctccttatt catatatatc cttgattacg
      541 atggcgatac agcaggcccc gtctaagatg ctgactctga gtgagatata ccagtggatc
      601 atggaccttt ttccttacta ccggcaaaac caacagagat ggcaaaactc aatacgccat
      661 agcctttcct tcaatgattg ctttgtcaaa gtcgctcgga gccctgacaa gcccggtaaa
      721 gggtcctatt ggacccttca tccagatagc ggcaatatgt tcgagaatgg ttgttatctt
      781 agacggcaga aacgattcaa atgtgagaaa cagccaggtg ccggcggtgg tggcggcagc
      841 ggttcaggcg gaagtggtgc caagggtggg cctgagtcta gaaaagaccc cagcggagca
      901 agcaatccaa gcgcggactc tcccctgcac cgcggtgttc atggtaagac aggtcagctt
      961 gagggggcgc ctgctccagg cccggctgcg tcaccgcaaa cactggacca tagtggagct
     1021 acagcgaccg gaggtgcttc agaactcaag acgcctgcgt cctccactgc gcctccgatc
     1081 tccagtggtc ccggtgcact tgcctctgtt cctgcatctc atccagcaca cggactcgcg
     1141 ccgcacgagt cccagctcca tttgaaaggg gacccacact acagctttaa ccacccattc
     1201 tctattaaca atttgatgtc atcctcagaa cagcagcata aactcgactt caaagcctat
     1261 gaacaggccc tgcagtattc tccatatggc tctacacttc ctgcttctct tccattgggg
     1321 tctgcaagtg tgacaacgcg ctccccaatc gagccaagtg ccctcgagcc tgcttattat
     1381 caaggagtat attcccgacc agttttgaat acaagt
//
