LOCUS       sequence-228685-        1374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express FOXA2 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_FOXA2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgctgggag cggtgaagat ggaagggcac gagccgtccg actggagcag ctactatgca
       61 gagcccgagg gctactcctc cgtgagcaac atgaacgccg gcctggggat gaacggcatg
      121 aacacgtaca tgagcatgtc ggcggccgcc atgggcagcg gctcgggcaa catgagcgcg
      181 ggctccatga acatgtcgtc gtacgtgggc gctggcatga gcccgtccct ggcggggatg
      241 tcccccggcg cgggcgccat ggcgggcatg ggcggctcgg ccggggcggc tggcgtggcg
      301 ggcatggggc cgcacttgag tcccagcctg agcccgctcg gggggcaggc ggccggggcc
      361 atgggcggcc tggcccccta cgccaacatg aactccatga gccccatgta cgggcaggcg
      421 ggcctgagcc gcgcccgcga ccccaagacc tacaggcgca gctacacgca cgcaaagccg
      481 ccctactcgt acatctcgct catcaccatg gccatccagc agagccccaa caagatgctg
      541 acgctgagcg agatctacca gtggatcatg gacctcttcc ccttctaccg gcagaaccag
      601 cagcgctggc agaactccat ccgccactcg ctctccttca acgactgttt cctgaaggtg
      661 ccccgctcgc ccgacaagcc cggcaagggc tccttctgga ccctgcaccc tgactcgggc
      721 aacatgttcg agaacggctg ctacctgcgc cgccagaagc gcttcaagtg cgagaagcag
      781 ctggcgctga aggaggccgc aggcgccgcc ggcagcggca agaaggcggc cgccggggcc
      841 caggcctcac aggctcaact cggggaggcc gccgggccgg cctccgagac tccggcgggc
      901 accgagtcgc ctcactcgag cgcctccccg tgccaggagc acaagcgagg gggcctggga
      961 gagctgaagg ggacgccggc tgcggcgctg agccccccag agccggcgcc ctctcccggg
     1021 cagcagcagc aggccgcggc ccacctgctg ggcccgcccc accacccggg cctgccgcct
     1081 gaggcccacc tgaagccgga acaccactac gccttcaacc acccgttctc catcaacaac
     1141 ctcatgtcct cggagcagca gcaccaccac agccaccacc accaccagcc ccacaaaatg
     1201 gacctcaagg cctacgaaca ggtgatgcac taccccggct acggttcccc catgcctggc
     1261 agcttggcca tgggcccggt cacgaacaaa acgggcctgg acgcctcgcc cctggccgca
     1321 gatacctcct actaccaggg ggtgtactcc cggcccatta tgaactcctc tttg
//
