LOCUS       sequence-22870-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  TITLE     Zebrafish Expression Plasmids
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZE12 sonic hedgehog CMV
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        224..242
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     224..241
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     255..274
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 tgaaccgtca gaacgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
       61 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
      121 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
      181 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
      241 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
      301 cggttctatc gattgaattc cccggggatc tgattcgaga acaccgcagc gagattcagc
      361 gtgcggcaaa atgcggcttt tgacgagagt gctgctggtg tctcttctca ctctgtcctt
      421 ggtggtgtcc ggactggcct gcggtcctgg cagaggctac ggcagaagaa gacatccgaa
      481 gaagctgaca cctctcgcct acaagcagtt catacctaat gtcgcggaga agaccttagg
      541 ggccagcggc agatacgagg gcaagataac gcgcaattcg gagagattta aagaacttac
      601 tccaaattat aatcccgaca ttatctttaa ggatgaggag aacacgggag c
//
