LOCUS       sequence-228700-        1326 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express GATA4 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_GATA4_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtaccaga gcctggctat ggctgctaat catggacctc cccctggagc ctatgaagcc
       61 ggaggacctg gcgcttttat gcatggagct ggcgccgctt cttctcccgt gtatgtgcct
      121 acacctagag tgcccagcag cgtgctgggc ctttcttatc ttcagggagg aggagcagga
      181 tctgcttctg gcggagcttc aggcggatct tctggaggcg ctgcttcagg tgctggacct
      241 ggaactcaac agggatctcc tggatggtca caggcaggag ctgatggagc cgcttatacc
      301 cctcctcctg tgagccccag gtttagcttt cctggcacaa caggctcttt agctgccgct
      361 gctgctgcag ccgcagctag agaagcagct gcatattcta gtggcggagg agctgctgga
      421 gccggcttag ctggaagaga gcagtacgga agagccggat ttgccggaag ctatagcagc
      481 ccttaccctg cctatatggc cgatgttggc gcatcttggg cagccgccgc agcagcttct
      541 gcaggacctt ttgactcacc tgtgcttcac tctctgcctg gcagagctaa tcctgccgcc
      601 agacatccca acctggacat gttcgacgac ttcagcgagg gcagagaatg cgtgaactgc
      661 ggagccatga gcacccccct ttggagaaga gacggcaccg gccactacct ttgcaatgcc
      721 tgtggcctgt accacaagat gaacggcatc aacagacccc tgatcaagcc ccagagaaga
      781 ctgagcgcta gcagaagagt gggcctgtcc tgcgccaatt gccagaccac aaccaccaca
      841 ctgtggagga gaaatgccga gggcgagcct gtgtgtaacg cctgtggact gtacatgaag
      901 ctgcacggcg tgcccagacc tctggccatg agaaaggagg gcatccagac cagaaagaga
      961 aagcccaaga acctgaacaa gagcaagacc cccgctgctc cttctggaag cgagagcctg
     1021 cctccagcct ctggagccag cagcaatagc tctaacgcca ccacatcttc ttctgaggag
     1081 atgaggccca tcaaaaccga gccaggcctg agcagccact acggccacag ctctagcgtg
     1141 agccagactt ttagcgtgtc tgccatgtca ggccacggac ctagcattca ccctgtgctg
     1201 agcgccctga agttgagccc acagggctat gcttctcctg tgtctcagag ccctcagacc
     1261 tccagcaagc aggactcctg gaattctctg gtgctggccg acagccacgg cgatatcatc
     1321 accgcc
//
