LOCUS       sequence-228709-         540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express HAND2 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_HAND2_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagtctgg taggtggttt tccccaccac ccggtggtgc accacgaggg ctacccgttt
       61 gccgccgccg ccgccgccag ccgctgcagc catgaggaga acccctactt ccatggctgg
      121 ctcatcggcc accccgagat gtcgcccccc gactacagca tggccctgtc ctacagcccc
      181 gagtatgcca gcggcaccgc caaccgcaag gagcggcgca ggactcagag catcaacagc
      241 gccttcgccg aactgcgcga gtgcatcccc aacgtacccg ccgacaccaa actctccaaa
      301 atcaagaccc tgcgcctggc caccagctac atcgcctacc tcatggacct gctggccaag
      361 gacgaccaga atggcgaggc ggaggccttc aaggcagaga tcaagaagac cgacgtgaaa
      421 gaggagaaga ggaagaagga gctgaacgaa atcttgaaaa gcacagtgag cagcaacgac
      481 aagaaaacca aaggccggac gggctggccg cagcacgtct gggccctgga gctcaagcag
//
