LOCUS       sequence-228718-        1251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express HNF4A in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_HNF4A_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcgactct ccaaaaccct cgtcgacatg gacatggccg actacagtgc tgcactggac
       61 ccagcctaca ccaccctgga atttgagaat gtgcaggtgt tgacgatggg caatgacacg
      121 tccccatcag aaggcaccaa cctcaacgcg cccaacagcc tgggtgtcag cgccctgtgt
      181 gccatctgcg gggaccgggc cacgggcaaa cactacggtg cctcgagctg tgacggctgc
      241 aagggcttct tccggaggag cgtgcggaag aaccacatgt actcctgcag atttagccgg
      301 cagtgcgtgg tggacaaaga caagaggaac cagtgccgct actgcaggct caagaaatgc
      361 ttccgggctg gcatgaagaa ggaagccgtc cagaatgagc gggaccggat cagcactcga
      421 aggtcaagct atgaggacag cagcctgccc tccatcaatg cgctcctgca ggcggaggtc
      481 ctgtcccgac agatcacctc ccccgtctcc gggatcaacg gcgacattcg ggcgaagaag
      541 attgccagca tcgcagatgt gtgtgagtcc atgaaggagc agctgctggt tctcgttgag
      601 tgggccaagt acatcccagc tttctgcgag ctccccctgg acgaccaggt ggccctgctc
      661 agagcccatg ctggcgagca cctgctgctc ggagccacca agagatccat ggtgttcaag
      721 gacgtgctgc tcctaggcaa tgactacatt gtccctcggc actgcccgga gctggcggag
      781 atgagccggg tgtccatacg catccttgac gagctggtgc tgcccttcca ggagctgcag
      841 atcgatgaca atgagtatgc ctacctcaaa gccatcatct tctttgaccc agatgccaag
      901 gggctgagcg atccagggaa gatcaagcgg ctgcgttccc aggtgcaggt gagcttggag
      961 gactacatca acgaccgcca gtatgactcg cgtggccgct ttggagagct gctgctgctg
     1021 ctgcccacct tgcagagcat cacctggcag atgatcgagc agatccagtt catcaagctc
     1081 ttcggcatgg ccaagattga caacctgttg caggagatgc tgctgggagg tccgtgccaa
     1141 gcccaggagg ggcggggttg gagtggggac tccccaggag acaggcctca cacagtgagc
     1201 tcacccctca gctccttggc ttccccactg tgccgctttg ggcaagttgc t
//
