LOCUS       sequence-228721-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express HOXA1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_HOXA1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             193..222
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atggacaacg cgcggatgaa ttccttcctc gagtacccaa ttttgtctag tggagacagt
       61 ggcacttgca gtgcccgagc ctatccatca gaccacagaa ttacaacatt ccaaagctgt
      121 gcggtgtcag ccaacagttg cggcggagac gaccgcttcc tggtcggaag aggggttcaa
      181 attggatcac ctcaccatca ccatcaccac caccatcacc acccccaacc ggcgacttac
      241 caaaccagcg gcaatttggg cgtgagctat agccattcct catgtggacc ttcctatggg
      301 tctcagaatt tctccgcccc ttatagccca tacgccctga accaagaggc cgatgtatca
      361 ggaggctatc cccagtgcgc gccagcggtt tactcaggta atctttctag cccgatggtc
      421 cagcaccacc atcaccatca aggttatgcc ggcggtgcag tcggatcccc acaatacata
      481 caccatagtt acggccaaga gcaccaatcc ctggccctcg ctacatataa caactcactg
      541 tctccgcttc atgcttccca ccaagaagct tgtcggagtc ccgcctcaga aacttcctct
      601 ccagctcaga cttttgattg gatgaaggtc aagcggaatc cgcctaaaac gggcaaagta
      661 ggtgaatatg gctatttggg acagcctaat gctgtccgca ccaatttcac aacaaaacag
      721 cttactgaac tcgagaagga atttcatttt aataagtatt tgactcgagc gagacgagtc
      781 gaaatcgccg ctagtcttca acttaacgag acccaggtta agatatggtt ccagaacaga
      841 agaatgaaac aaaaaaagcg ggagaaggaa ggactcctcc ctatatcacc agccacaccc
      901 ccaggtaacg acgagaaggc ggaggaatct tcagagaaga gttccagctc cccttgtgtt
      961 ccttctcctg gtagctcaac cagcgatacc ctcacgacga gtcac
//
