LOCUS       sequence-228733-        1410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF4 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF4_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggctgtca gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga
       61 agggagaaga cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc
      121 cacatgaagc gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc
      181 gtggccacag acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg
      241 cccctacctc ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc
      301 tccaattcgc tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca
      361 gcctcctctt cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc
      421 ttcacctatc cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga
      481 ggcctcctct atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac
      541 atcaacgacg tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac
      601 ccggtgtaca ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc
      661 gtgctgaagg cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc
      721 gtcagcaaag gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg
      781 ccgccgcgca cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc
      841 gctggacccc ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg
      901 cagctcccca gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac
      961 tgtcaccctg ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca
     1021 tccttcctgc ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg
     1081 ccacccggtt cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc
     1141 cggaaaagga ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag
     1201 agttcccatc tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac
     1261 tgggacggct gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa
     1321 cacacggggc accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac
     1381 cacctcgcct tacacatgaa gaggcatttt
//
