LOCUS       sequence-228736-        1236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express LHX3 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_LHX3_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggaggcgc gcggggagct gggcccggcc cgggagtcgg cgggaggcga cctgctgcta
       61 gcactgctgg cgcggagggc ggacctgcgc cgagagatcc cgctgtgcgc tggctgtgac
      121 cagcacatcc tggaccgctt catcctcaag gctctggacc gccactggca cagcaagtgt
      181 ctcaagtgca gcgactgcca cacgccactg gccgagcgct gcttcagccg aggggagagc
      241 gtttactgca aggacgactt tttcaagcgc ttcgggacca agtgcgccgc gtgccagctg
      301 ggcatcccgc ccacgcaggt ggtgcgccgc gcccaggact tcgtgtacca cctgcactgc
      361 tttgcctgcg tcgtgtgcaa gcggcagctg gccacgggcg acgagttcta cctcatggag
      421 gacagccggc tcgtgtgcaa ggcggactac gaaaccgcca agcagcgaga ggccgaggcc
      481 acggccaagc ggccgcgcac gaccatcacc gccaagcagc tggagacgct gaagagcgct
      541 tacaacacct cgcccaagcc ggcgcgccac gtgcgcgagc agctctcgtc cgagacgggc
      601 ctggacatgc gcgtggtgca ggtttggttc cagaaccgcc gggccaagga gaagaggctg
      661 aagaaggacg ccggccggca gcgctggggg cagtatttcc gcaacatgaa gcgctcccgc
      721 ggcggctcca agtcggacaa ggacagcgtt caggaggggc aggacagcga cgctgaggtc
      781 tccttccccg atgagccttc cttggcggaa atgggcccgg ccaatggcct ctacgggagc
      841 ttgggggaac ccacccaggc cttgggccgg ccctcgggag ccctgggcaa cttctccctg
      901 gagcatggag gcctggcagg cccagagcag taccgagagc tgcgtcccgg cagcccctac
      961 ggtgtccccc catcccccgc cgccccgcag agcctccctg gcccccagcc cctcctctcc
     1021 agcctggtgt acccagacac cagcttgggc cttgtgccct cgggagcccc cggcgggccc
     1081 ccacccatga gggtgctggc agggaacgga cccagttctg acctatccac ggggagcagc
     1141 gggggttacc ccgacttccc tgccagcccc gcctcctggc tggatgaggt agaccacgct
     1201 cagttctcag gcctcatggg cccagctttc ttgtac
//
