LOCUS       sequence-228742-        1419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MEF2C in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MEF2C_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggggagaa aaaagattca gattacgagg attatggatg aacgtaacag acaggtgaca
       61 tttacaaaga ggaaatttgg gttgatgaag aaggcttatg agctgagcgt gctgtgtgac
      121 tgtgagattg cgctgatcat cttcaacagc accaacaagc tgttccagta tgccagcacc
      181 gacatggaca aagtgcttct caagtacacg gagtacaacg agccgcatga gagccggaca
      241 aactcagaca tcgtggagac gttgagaaag aagggcctta atggctgtga cagcccagac
      301 cccgatgcgg acgattccgt aggtcacagc cctgagtctg aggacaagta caggaaaatt
      361 aacgaagata ttgatctaat gatcagcagg caaagattgt gtgctgttcc acctcccaac
      421 ttcgagatgc cagtctccat cccagtgtcc agccacaaca gtttggtgta cagcaaccct
      481 gtcagctcac tgggaaaccc caacctattg ccactggctc acccttctct gcagaggaat
      541 agtatgtctc ctggtgtaac acatcgacct ccaagtgcag gtaacacagg tggtctgatg
      601 ggtggagacc tcacgtctgg tgcaggcacc agtgcaggga acgggtatgg caatccccga
      661 aactcaccag gtctgctggt ctcacctggt aacttgaaca agaatatgca agcaaaatct
      721 cctcccccaa tgaatttagg aatgaataac cgtaaaccag atctccgagt tcttattcca
      781 ccaggcagca agaatacgat gccatcagtg tctgaggatg tcgacctgct tttgaatcaa
      841 aggataaata actcccagtc ggctcagtca ttggctaccc cagtggtttc cgtagcaact
      901 cctactttac caggacaagg aatgggagga tatccatcag ccatttcaac aacatatggt
      961 accgagtact ctctgagtag tgcagacctg tcatctctgt ctgggtttaa caccgccagc
     1021 gctcttcacc ttggttcagt aactggctgg caacagcaac acctacataa catgccacca
     1081 tctgccctca gtcagttggg agcttgcact agcactcatt tatctcagag ttcaaatctc
     1141 tccctgcctt ctactcaaag cctcaacatc aagtcagaac ctgtttctcc tcctagagac
     1201 cgtaccacca ccccttcgag atacccacaa cacacgcgcc acgaggcggg gagatctcct
     1261 gttgacagct tgagcagctg tagcagttcg tacgacggga gcgaccgaga ggatcaccgg
     1321 aacgaattcc actcccccat tggactcacc agaccttcgc cggacgaaag ggaaagtccc
     1381 tcagtcaagc gcatgcgact ttctgaagga tgggcaaca
//
