LOCUS       sequence-228745-         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MESP1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MESP1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcccagc ccctgtgccc gccgctctcc gagtcctgga tgctctctgc ggcctggggc
       61 ccaactcggc ggccgccgcc ctccgacaag gactgcggcc gctccctcgt ctcgtcccca
      121 gactcatggg gcagcacccc agccgacagc cccgtggcga gccccgcgcg gccaggcacc
      181 ctccgggacc cccgcgcccc ctccgtaggt aggcgcggcg cgcgcagcag ccgcctgggc
      241 agcgggcaga ggcagagcgc cagtgagcgg gagaaactgc gcatgcgcac gctggcccgc
      301 gccctgcacg agctgcgccg ctttctaccg ccgtccgtgg cgcccgcggg ccagagcctg
      361 accaagatcg agacgctgcg cctggctatc cgctatatcg gccacctgtc ggccgtgcta
      421 ggcctcagcg aggagagtct ccagcgccgg tgccggcagc gcggtgacgc ggggtcccct
      481 cggggctgcc cgctgtgccc cgacgactgc cccgcgcaga tgcagacacg gacgcaggct
      541 gaggggcagg ggcaggggcg cgggctgggc ctggtatccg ccgtccgcgc cggggcgtcc
      601 tggggatccc cgcctgcctg ccccggagcc cgagctgcac ccgagccgcg cgacccgcct
      661 gcgctgttcg ccgaggcggc gtgcccggaa gggcaggcga tggagccaag cccaccgtcc
      721 ccgctccttc cgggcgacgt gctggctctg ttggagacct ggatgcccct ctcgcctctg
      781 gagtggctgc ctgaggagcc caagttg
//
