LOCUS       sequence-228748-        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MITF in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MITF_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgctggaaa tgctagaata taatcactat caggtgcaga cccacctcga aaaccccacc
       61 aagtaccaca tacagcaagc ccaacggcag caggtaaagc agtacctttc taccacttta
      121 gcaaataaac atgccaacca agtcctgagc ttgccatgtc caaaccagcc tggcgatcat
      181 gtcatgccac cggtgccggg gagcagcgca cccaacagcc ccatggctat gcttacgctt
      241 aactccaact gtgaaaaaga gggattttat aagtttgaag agcaaaacag ggcagagagc
      301 gagtgcccag gcatgaacac acattcacga gcgtcctgta tgcagatgga tgatgtaatc
      361 gatgacatca ttagcctaga atcaagttat aatgaggaaa tcttgggctt gatggatcct
      421 gctttgcaaa tggcaaatac gttgcctgtc tcgggaaact tgattgatct ttatggaaac
      481 caaggtctgc ccccaccagg cctcaccatc agcaactcct gtccagccaa ccttcccaac
      541 ataaaaaggg agctcacaga gtctgaagca agagcactgg ccaaagagag gcagaaaaag
      601 gacaatcaca acctgattga acgaagaaga agatttaaca taaatgaccg cattaaagaa
      661 ctaggtactt tgattcccaa gtcaaatgat ccagacatgc gctggaacaa gggaaccatc
      721 ttaaaagcat ccgtggacta tatccgaaag ttgcaacgag aacagcaacg cgcaaaagaa
      781 cttgaaaacc gacagaagaa actggagcac gccaaccggc atttgttgct cagaatacag
      841 gaacttgaaa tgcaggctcg agctcatgga ctttccctta ttccatccac gggtctctgc
      901 tctccagatt tggtgaatcg gatcatcaag caagaacccg ttcttgagaa ctgcagccaa
      961 gacctccttc agcatcatgc agacctaacc tgtacaacaa ctctcgatct cacggatggc
     1021 accatcacct tcaacaacaa cctcggaact gggactgagg ccaaccaagc ctatagtgtc
     1081 cccacaaaaa tgggatccaa actggaagac atcctgatgg acgacaccct ttctcccgtc
     1141 ggtgtcactg atccactcct ttcctcagtg tcccccggag cttccaaaac aagcagccgg
     1201 aggagcagta tgagcatgga agagacggag cacacttgt
//
