LOCUS       sequence-228757-        1392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MYCN in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYCN_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccgagtt gttccacgtc tacgatgcca ggaatgatat gcaagaaccc cgacttggag
       61 tttgactctt tgcaaccatg cttttatccg gatgaagacg acttttattt cggcggcccg
      121 gacagcaccc ctcctggaga ggacatctgg aaaaaattcg aacttttgcc tacaccccca
      181 ctcagtccct ctcgaggatt tgcggaacac agcagtgaac cgccgtcttg ggtgacagag
      241 atgctcctcg agaacgaatt gtggggaagc cctgcggagg aagacgcttt cgggctcggt
      301 ggactcggag gtctcacgcc gaacccagtc atactgcagg attgcatgtg gtctggattc
      361 tcagctcggg agaagctgga acgggcagtt tctgagaaac tccaacatgg ccggggccct
      421 ccaacagcgg gttctaccgc acagtcccct ggtgctggag ccgctagtcc cgcggggaga
      481 ggccatgggg gcgcggcagg agcgggtagg gccggcgctg cgttgcctgc tgagcttgcg
      541 caccccgccg ctgaatgtgt agatcccgcg gtagtgtttc cgttccccgt taataagcga
      601 gaaccggcac cggtgccagc cgctcctgcg tctgcacccg cggcaggtcc tgctgtcgcc
      661 tcaggagcag gtattgccgc tcctgcaggg gcaccaggag tagcccctcc aaggcccggc
      721 ggtaggcaaa cctccggcgg cgaccacaaa gcactctcaa cgagcggaga ggatacactg
      781 tccgatagtg atgacgagga cgacgaagag gaggacgagg aggaggagat agatgttgtc
      841 acggtcgaga agcgaaggag ttcttcaaat acaaaagcgg taacgacatt cacgataaca
      901 gtaagaccta agaacgcagc cctcggtcca gggcgggccc agtccagtga gcttatactt
      961 aagcgctgcc tgccgattca ccagcagcat aactacgcgg cccctagtcc ctacgttgag
     1021 agcgaggatg cccccccaca aaaaaaaata aagtctgaag cgtccccccg ccccctgaaa
     1081 tccgtaatcc ccccaaaggc gaagtcactc agtcccagga attcagattc cgaggactcc
     1141 gaacggcggc ggaatcataa catacttgag agacaacgac gcaatgacct gaggtcttct
     1201 tttttgaccc tccgagatca cgtccccgag ctggttaaga atgagaaagc tgcgaaggta
     1261 gtcatactga aaaaggccac cgagtatgtc catagtttgc aagctgagga gcaccagctt
     1321 ctccttgaaa aggagaaact tcaggcacga caacagcaat tgctgaaaaa gattgagcat
     1381 gcacgcactt gt
//
