LOCUS       sequence-228760-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express MYOD1 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_MYOD1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagctac tgtcgccacc gctccgcgac gtagacctga cggcccccga cggctctctc
       61 tgctcctttg ccacaacgga cgacttctat gacgacccgt gtttcgactc cccggacctg
      121 cgcttcttcg aagacctgga cccgcgcctg atgcacgtgg gcgcgctcct gaaacccgaa
      181 gagcactcgc acttccccgc ggcggtgcac ccggccccgg gcgcacgtga ggacgagcat
      241 gtgcgcgcgc ccagcgggca ccaccaggcg ggccgctgcc tactgtgggc ctgcaaggcg
      301 tgcaagcgca agaccaccaa cgccgaccgc cgcaaggccg ccaccatgcg cgagcggcgc
      361 cgcctgagca aagtaaatga ggcctttgag acactcaagc gctgcacgtc gagcaatcca
      421 aaccagcggt tgcccaaggt ggagatcctg cgcaacgcca tccgctatat cgagggcctg
      481 caggctctgc tgcgcgacca ggacgccgcg ccccctggcg ccgcagccgc cttctatgcg
      541 ccgggcccgc tgcccccggg ccgcggcggc gagcactaca gcggcgactc cgacgcgtcc
      601 agcccgcgct ccaactgctc cgacggcatg atggactaca gcggcccccc gagcggcgcc
      661 cggcggcgga actgctacga aggcgcctac tacaacgagg cgcccagcga acccaggccc
      721 gggaagagtg cggcggtgtc gagcctagac tgcctgtcca gcatcgtgga gcgcatctcc
      781 accgagagcc ctgcggcgcc cgccctcctg ctggcggacg tgccttctga gtcgcctccg
      841 cgcaggcaag aggctgccgc ccccagcgag ggagagagca gcggcgaccc cacccagtca
      901 ccggacgccg ccccgcagtg ccctgcgggt gcgaacccca acccgatata ccaggtgctc
//
