LOCUS       sequence-228766-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express NEUROD1 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_NEUROD1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaccaaat cgtacagcga gagtgggctg atgggcgagc ctcagcccca aggtcctcca
       61 agctggacag acgagtgtct cagttctcag gacgaggagc acgaggcaga caagaaggag
      121 gacgacctcg aagccatgaa cgcagaggag gactcactga ggaacggggg agaggaggag
      181 gacgaagatg aggacctgga agaggaggaa gaagaggaag aggaggatga cgatcaaaag
      241 cccaagagac gcggccccaa aaagaagaag atgactaagg ctcgcctgga gcgttttaaa
      301 ttgagacgca tgaaggctaa cgcccgggag cggaaccgca tgcacggact gaacgcggcg
      361 ctagacaacc tgcgcaaggt ggtgccttgc tattctaaga cgcagaagct gtccaaaatc
      421 gagactctgc gcttggccaa gaactacatc tgggctctgt cggagatcct gcgctcaggc
      481 aaaagcccag acctggtctc cttcgttcag acgctttgca agggcttatc ccaacccacc
      541 accaacctgg ttgcgggctg cctgcaactc aatcctcgga cttttctgcc tgagcagaac
      601 caggacatgc ccccccacct gccgacggcc agcgcttcct tccctgtaca cccctactcc
      661 taccagtcgc ctgggctgcc cagtccgcct tacggtacca tggacagctc ccatgtcttc
      721 cacgttaagc ctccgccgca cgcctacagc gcagcgctgg agcccttctt tgaaagccct
      781 ctgactgatt gcaccagccc ttcctttgat ggacccctca gcccgccgct cagcatcaat
      841 ggcaacttct ctttcaaaca cgaaccgtcc gccgagtttg agaaaaatta tgcctttacc
      901 atgcactatc ctgcagcgac actggcaggg gcccaaagcc acggatcaat cttctcaggc
      961 accgctgccc ctcgctgcga gatccccata gacaatatta tgtccttcga tagccattca
     1021 catcatgagc gagtcatgag tgcccagctc aatgccatat ttcatgat
//
