LOCUS       sequence-228769-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express NEUROG1 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_NEUROG1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgccagccc gccttgagac ctgcatctcc gacctcgact gcgccagcag cagcggcagt
       61 gacctatccg gcttcctcac cgacgaggaa gactgtgcca gactccaaca ggcagcctcc
      121 gcttcggggc cgcccgcgcc ggcccgcagg ggcgcgccca atatctcccg ggcgtctgag
      181 gttccagggg cacaggacga cgagcaggag aggcggcggc gccgcggccg gacgcgggtc
      241 cgctccgagg cgctgctgca ctcgctgcgc aggagccggc gcgtcaaggc caacgatcgc
      301 gagcgcaacc gcatgcacaa cttgaacgcg gccctggacg cactgcgcag cgtgctgccc
      361 tcgttccccg acgacaccaa gctcaccaaa atcgagacgc tgcgcttcgc ctacaactac
      421 atctgggctc tggccgagac actgcgcctg gcggatcaag ggctgcccgg aggcggtgcc
      481 cgggagcgcc tcctgccgcc gcagtgcgtc ccctgcctgc ccggtccccc aagccccgcc
      541 agcgacgcgg agtcctgggg ctcaggtgcc gccgccgcct ccccgctctc tgaccccagt
      601 agcccagccg cctccgaaga cttcacctac cgccccggcg accctgtttt ctccttccca
      661 agcctgccca aagacttgct ccacacaacg ccctgtttca ttccttacca c
//
