LOCUS       sequence-228772-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express NEUROG3 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_NEUROG3_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacaccac aaccatctgg tgctcccaca gtccaggtga cgcgagagac tgaaagatca
       61 ttcccacgcg cgtccgagga tgaggtgaca tgtccaacta gcgcaccccc ctctcctacc
      121 cggacccgcg ggaattgtgc tgaggccgaa gagggaggat gcagaggagc accaaggaaa
      181 cttcgagccc gacggggtgg aagaagccgc cccaagtctg agctcgccct tagcaagcag
      241 cgccgcagtc ggaggaaaaa ggcaaacgac cgggaaagga ataggatgca taatcttaat
      301 tctgctctgg acgctctgcg aggcgtactt cctactttcc cggatgacgc gaaattgacc
      361 aagatagaga ctctccggtt tgcacataat tacatctggg ctcttacaca aacactgaga
      421 attgccgatc acagtcttta cgctcttgag ccacccgccc cgcactgtgg cgagctgggt
      481 agccccggcg gctctcctgg agactggggg tctttgtatt ctcctgtcag ccaagcggga
      541 tctttgagtc cggctgccag tctcgaagaa agacccggac tccttggagc gactttttca
      601 gcatgtctgt cccctggctc attggctttc tcagactttt tg
//
