LOCUS       sequence-228775-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express NRL in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_NRL_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(713..737)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggccctgc ctcccagccc gctggccatg gaatatgtca atgactttga cttgatgaag
       61 tttgaggtaa agcgggaacc ctctgagggc cgacctggcc cacctacagc ctcactggga
      121 tccacacctt acagctcagt gcctccttca cccaccttca gtgaaccagg catggtaggg
      181 gcaaccgagg gtacacgacc aggtttggag gagctgtact ggcttgctac cctgcagcag
      241 cagcttgggg ctggggaggc attgggactg agtcctgaag aggccatgga gctactgcaa
      301 ggtcagggcc cagtccctgt tgatggaccc catggttact acccagggag cccagaggag
      361 acaggagccc agcacgttca gttggcagag cggttttccg acgcggcgct tgtctcgatg
      421 tctgtgcgag aactaaaccg gcagctgcgg ggatgcggga gagacgaggc tctacgactg
      481 aagcagaggc gtcgaacgct gaagaaccgt ggctatgcgc aagcatgtcg ttccaagagg
      541 ctgcaacaga ggcgaggtct tgaggccgag cgcgcccgtc ttgcagccca gctagatgcg
      601 ctacgagctg aagtagcacg tttggcaaga gagcgagatc tctacaaggc tcgctgtgac
      661 cggctaacct cgagtggccc cgggtccggg gatccctccc accttttcct ctgcccaact
      721 ttcttgtaca aagttgtccc c
//
