LOCUS       sequence-228778-        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express ONECUT1 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_ONECUT1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             370..399
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             373..402
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atgaacgcgc agctgaccat ggaagcgatc ggcgagctgc acggggtgag ccatgagccg
       61 gtgcccgccc ctgccgacct gctgggcggc agcccccacg cgcgcagctc cgtggcgcac
      121 cgcggcagcc acctgccccc cgcgcacccg cgctccatgg gcatggcgtc cctgctggac
      181 ggcggcagcg gcggcggaga ttaccaccac caccaccggg cccctgagca cagcctggcc
      241 ggccccctgc atcccaccat gaccatggcc tgcgagactc ccccaggtat gagcatgccc
      301 accacctaca ccaccttgac ccctctgcag ccgctgcctc ccatctccac agtctcggac
      361 aagttccccc accatcacca ccaccaccat caccaccacc acccgcacca ccaccagcgc
      421 ctggcgggca acgtgagcgg tagcttcacg ctcatgcggg atgagcgcgg gctggcctcc
      481 atgaataacc tctatacccc ctaccacaag gacgtggccg gcatgggcca gagcctctcg
      541 cccctctcca gctccggtct gggcagcatc cacaactccc agcaagggct cccccactat
      601 gcccacccgg gggccgccat gcccaccgac aagatgctca cccccaacgg cttcgaagcc
      661 caccacccgg ccatgctcgg ccgccacggg gagcagcacc tcacgcccac ctcggccggc
      721 atggtgccca tcaacggcct tcctccgcac catccccacg cccacctgaa cgcccagggc
      781 cacgggcaac tcctgggcac agcccgggag cccaaccctt cggtgaccgg cgcgcaggtc
      841 agcaatggaa gtaattcagg gcagatggaa gagatcaata ccaaagaggt ggcgcagcgt
      901 atcaccaccg agctcaagcg ctacagcatc ccacaggcca tcttcgcgca gagggtgctc
      961 tgccgctccc aggggaccct ctcggacctg ctgcgcaacc ccaaaccctg gagcaaactc
     1021 aaatccggcc gggagacctt ccggaggatg tggaagtggc tgcaggagcc ggagttccag
     1081 cgcatgtccg cgctccgctt agcagcatgc aaaaggaaag aacaagaaca tgggaaggat
     1141 agaggcaaca cacccaaaaa gcccaggttg gtcttcacag atgtccagcg tcgaactcta
     1201 catgcaatat tcaaggaaaa taagcgtcca tccaaagaat tgcaaatcac catttcccag
     1261 cagctggggt tggagctgag cactgtcagc aacttcttca tgaacgcaag aaggaggagt
     1321 ctggacaagt ggcaggacga gggcagctcc aattcaggca actcatcttc ttcatcaagc
     1381 acttgtacca aagca
//
